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pe buffer  (Sangon Biotech)

 
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    Sangon Biotech pe buffer
    Pe Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+buffer/buffer+pe/pm41260246-246-22-44
    Average 86 stars, based on 1 article reviews
    pe buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Ribosomal protein bL31c interacts with translation elongation factor RAB8D to regulate chloroplast translation elongation and PSI-LHCI-LHCII assembly in Arabidopsis.
    Article Snippet: For confocal microscopy (TCS SP8, Leica) capture, 488 the YFP signal was excited at 514 nm and collected between 520 and 540 nm. .. 489 Jo urn al Pr e-p roo f 19 Ribosomal complex isolation and protein analyses 490 The tissue powder was dissolved in PE buffer (50 mM Tris-HCL at pH 7.5, 100 mM KCl, 10 491 mM MgCl2, 5 mM EGTA, 1 mM dithiothreitol (DTT, Sangon Biotech, A620058), 1×cocktail, 492 50 μM phenylmethyl sulfonyl fluoride (PMSF, Sangon Biotech, A610425), 1×RNase inhibitor 493 (Vazyme, R301-01), 0.5% Triton-X100, 0.2% β-dodecyl maltoside (DM, Sangon Biotech, 494 A610424), 0.2% sodium deoxycholate (DOC, Sigma, 302-95-4, 30970) and 0.2% 495 polyoxyethylene lauryl ether (Brij-35, Sangon Biotech, A610217)) and centrifuged at 10 000×g 496 for 15 min at 4°C. ..



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    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Expressing, Western Blot, Staining, Control

    The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Biomarker Discovery, Flow Cytometry, Endpoint Dilution Assay, Expressing, Immunofluorescence, Staining, Knockdown, Fluorescence