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exp pbc001 pcr barcoding kit  (New England Biolabs)


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    Structured Review

    New England Biolabs exp pbc001 pcr barcoding kit
    Exp Pbc001 Pcr Barcoding Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+barcoding+kit/Quick+Ligation+Kit/pm40848661-83-4-17
    Average 99 stars, based on 2362 article reviews
    exp pbc001 pcr barcoding kit - by Bioz Stars, 2026-09
    99/100 stars

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    Ligation:

    Article Title: Non-destructive transcriptomics via vesicular export
    Article Snippet: For assembly, DNA concentrations were quantified via spectrophotometry (NanoDrop 1000, Thermo Fisher Scientific). .. Ligation reactions (10 μL) utilized 30–50 ng of the origin-containing backbone and a 1:1–3 molar ratio of backbone to insert, facilitated by the Quick Ligation Kit (NEB) for 5–10 min at room temperature. ..

    Article Title: Non-destructive transcriptomics via vesicular export.
    Article Snippet: For assembly, DNA concentrations were quantified via spectrophotometry (NanoDrop 1000, Thermo Fisher Scientific). .. Ligation reactions (10 μL) utilized 30–50 ng of the origin-containing backbone and a 1:1–3 molar ratio of backbone to insert, facilitated by the Quick Ligation Kit (NEB) for 5–10min at room temperature. ..

    Article Title: Highly sensitive in vitro assays to define substrate preferences and sites of nucleic-acid binding, modifying, and cleaving agents
    Article Snippet: .. To achieve this, we supply adapter in 10-fold molar excess over library fragments and ligate using the NEB quick ligation kit, incubating the reaction at 25° C. for 10 min. 9. ..

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
    Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    Plasmid Preparation:

    Article Title: Temporal analysis of two inducible human genes reveals post-transcriptional H3K4me3 deposition
    Article Snippet: .. The resulting duplexes were ligated into the pLKO.1- vector (Addgene# 8453), linearized with Age I and EcoR I, using the Quick T4 DNA Ligase (Cat# M2200L; NEB) kit. .. HEK293T cells were transfected with either sh MLL1 or an empty vector (control) for lentiviral production along with third-generation lenti-packaging plasmids pREV, pRRE, and pMD2.G using Xfect Transfection Reagent (Cat# 631318; Takara) based on the manufacturer’s protocol.

    Purification:

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
    Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    Phospho-proteomics:

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
    Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

    Recombinant:

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    High Performance Liquid Chromatography:

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    Polymerase Chain Reaction:

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    Real-time Polymerase Chain Reaction:

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    Hybridization:

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..

    Software:

    Article Title: The shared genomic history of Middle- to Late-Holocene populations from the Southern Cone of South America.
    Article Snippet: 20 mg/ml) NEB B9200S ROTIPHORESE®50x TAE Buffer, 2.5 l, glass, 1 x 2.5 l Carl Roth CL86.3 Sodium acetate buffer solution, 3 M, pH 5,2 Merck S7899-500ML Sodium chloride solution 5 M Merck S5150-1L Sodium hypochlorite solution, 2.5 l, 12 % Cl, stabilised, technical Carl Roth 9062.4 Sodium phosphat, 0.5 M-Pufferlösung, pH 7.0 Thermo Fisher Scientific J63791.AP SpeedBeads magnetic carboxylate modified particles Merck GE65152105050250 SSC (20X), RNase-frei, 500 ml Thermo Fisher Scientific AM9763 T4 DNA Ligase (5 U/μl) Thermo Fisher Scientific EL0012 T4 DNA Ligase HC (30 U/μl) Thermo Fisher Scientific EL0013 T4 DNA Polymerase 3U/μl NEB M0203L (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–15.e1–e14, May 4, 2026 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER T4 RNA Ligase Reaction Buffer + PEG 8000 + ATP 10 mM NEB B0216L T4-Polynukleotid-Kinase (10 U/μl) Thermo Fisher Scientific EK0032 Tango-Buffer (10X) Life Technologies BY5 TE-Buffer low EDTA, pH 8.0 VWR A8569.0500 TRIS Buffer solution pH 8 (1 mol/l) VWR A4577.1000 TWEEN® 20 Merck P9416-50ML UltraPureTM 1 M Tris-HCI, pH 8,0 Thermo Fisher Scientific 15568025 UltraPureTM Salmon Sperm DNA Solution Thermo Fisher Scientific 15632011 Uracil Glycosylase inhibitor (UGI) NEB M0281L USER Enzyme, recombinant NEB M5505L Water HPLC Plus Merck 34877-2.5L-M EDTA, 05M (pH 8.0) MolecularBiology Grade 100 ml Promega V4231 Proteinase K, Recombinant, PCR-Grade Roche 03115852001 N-Lauroylsarcosine Merck 8147150100 PE Buffer Qiagen 19065 EB Buffer Qiagen 19086 PlatinumTM SuperFi II PCR Master Mix Thermo Fisher Scientific 12368010 Illumina Purification Beads, 100 ml Illumina 20060057 QubitTM 1X dsDNA High Sensitivity (HS) Thermo Fisher Scientific Q33230 Critical commercial assays Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 15 ml Merck UFC900308 Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO, 4 ml Merck UFC800308 Biozym Blue S’Green qPCR Kit Separate ROX Biozym 331416XL D1000 ScreenTape Agilent 5067-5582 High Pure Viral Nucleic Acid Large Volume Kit, Roche CVU, Roche 05114403001 MinElute PCR Purification Kit (250) Qiagen 28006 Oligo aCGH/ChIP-on-chip Hybridization Kit Agilent 5188-5220 Quick Ligation Kit NEB M2200L TapeStation Test-Tape (TSTT) Agilent 5067-5601 High Sensitivity D1000 ScreenTape Agilent 5067-5582 High Sensitivity D1000 Reagents Agilent 5067-5583 D1000 ScreenTape Agilent 5067-5584 D1000 Reagents Agilent 5067-5585 15 ml Conical Reservoir Zymo Research C1031-25 Deposited data MtDNA and nuclear DNA sequences (European Nucleotide Archive) This study PRJEB102023 Software and algorithms EAGER version 1.92.38 https://github.com/apeltzer/EAGER-GUI Peltzer et al.124 AdapterRemoval https://github.com/MikkelSchubert/ adapterremoval Schubert et al.125 Burrows-Wheeler-Aligner (BWA) https://github.com/lh3/bwa Li and Durbin126 DeDup https://github.com/apeltzer/DeDup Peltzer et al.124 mapDamage2.0 https://ginolhac.github.io/mapDamage/ Jónsson et al.127 schmutzi https://github.com/grenaud/schmutzi Renaud et al.129 AngsD https://github.com/ANGSD/angsd Korneliussen et al.130 samtools trim https://github.com/samtools/samtools Danecek et al.132 pileupCaller https://github.com/stschiff/sequenceTools N/A (Continued on next page) ll OPEN ACCESS e3 Current Biology 36, 1–15.e1–e14, May 4, 2026 Article ..



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