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plasmid dna purification kit nucleobondvr pc 2000  (MACHEREY NAGEL)


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    Structured Review

    MACHEREY NAGEL plasmid dna purification kit nucleobondvr pc 2000
    Plasmid Dna Purification Kit Nucleobondvr Pc 2000, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc/NucleoBond+PC+2000%2C+Mega+kit+for+transfection-grade+plasmid+DNA/pm24771111-41-7-14
    Average 90 stars, based on 8 article reviews
    plasmid dna purification kit nucleobondvr pc 2000 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Development of Experimental Genetic Tools for Campylobacter fetus
    Article Snippet: .. Plasmids were purified routinely from E. coli by alkaline lysis ( 3 ); for large-scale preparations, the NucleoBond PC 2000 kit (Macherey-Nagel, Düren, Germany) or the PureYield Plasmid Midiprep system (Promega, Mannheim, Germany) was employed. .. Plasmid DNA from Campylobacter cells was isolated with the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany).

    Article Title: Fluorescence in situ hybridization and qPCR to detect Merkel cell polyomavirus physical status and load in Merkel cell carcinomas.
    Article Snippet: MCPyV DNA was isolated from a MCC and cloned into a StrataClone PCR Cloning Vector (pSC-A-amp/kan; Stratagene, Santa Clara, CA). .. The plasmid DNA was purified with the plasmid DNA Purification Kit NucleoBondVR PC 2000 (Macherey-Nagel, Dueren, Germany) and sequenced using T7 and T3 primers. .. The HPyV-6 and -7 probes were generated from the 24727 plasmid: pHPyV6-607a and 24728 plasmid: pHPyV7-13a, respectively (Addgene, Cambridge, MA).

    Article Title: Development of Experimental Genetic Tools for Campylobacter fetus
    Article Snippet: .. Plasmids were purified routinely from E. coli by alkaline lysis (3); for large-scale preparations, the NucleoBond PC 2000 kit (Macherey-Nagel, Düren, Germany) or the PureYield Plasmid Midiprep system (Promega, Mannheim, Germany) was employed. .. Plasmid DNA from Campylobacter cells was isolated with the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany).

    Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles
    Article Snippet: .. In short, the plasmid pMET7-gag-EGFP was purified from DH10B E. coli via the PC2000 nucleobond kit (Cat. No: MN 740576, Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions. .. For rEV generation, HEK293T cells were seeded in Falcon cell culture Multi-Flasks (Cat. No: 353144, Corning incorporated, Corning, NY, USA) and transiently transfected at 70–80% confluency using 25 kDa linear polyethyleneimine (PEI) (Cat. No: 23966, Polysciences, Niles, IL, USA) in a PEI:DNA ratio of 5:1 with a final concentration of 1 μg DNA/mL culture medium in a total volume of 120 mL per Multi-Flask.

    Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles.
    Article Snippet: .. In short, the plasmid pMET7-gag-EGFP was purified from DH10B E. coli via the PC2000 nucleobond kit (Cat. No: MN 740576, Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions. .. For rEV generation, HEK293T cells were seeded in Falcon cell culture Multi-Flasks (Cat. No: 353144, Corning incorporated, Corning, NY, USA) and transiently transfected at 70–80% confluency using 25 kDa linear polyethyleneimine (PEI) (Cat. No: 23966, Polysciences, Niles, IL, USA) in a PEI:DNA ratio of 5:1 with a final concentration of 1 μg DNA/mL culture medium in a total volume of 120 mL per Multi-Flask.

    Alkaline Lysis:

    Article Title: Development of Experimental Genetic Tools for Campylobacter fetus
    Article Snippet: .. Plasmids were purified routinely from E. coli by alkaline lysis ( 3 ); for large-scale preparations, the NucleoBond PC 2000 kit (Macherey-Nagel, Düren, Germany) or the PureYield Plasmid Midiprep system (Promega, Mannheim, Germany) was employed. .. Plasmid DNA from Campylobacter cells was isolated with the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany).

    Article Title: Development of Experimental Genetic Tools for Campylobacter fetus
    Article Snippet: .. Plasmids were purified routinely from E. coli by alkaline lysis (3); for large-scale preparations, the NucleoBond PC 2000 kit (Macherey-Nagel, Düren, Germany) or the PureYield Plasmid Midiprep system (Promega, Mannheim, Germany) was employed. .. Plasmid DNA from Campylobacter cells was isolated with the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany).

    Plasmid Preparation:

    Article Title: Development of Experimental Genetic Tools for Campylobacter fetus
    Article Snippet: .. Plasmids were purified routinely from E. coli by alkaline lysis ( 3 ); for large-scale preparations, the NucleoBond PC 2000 kit (Macherey-Nagel, Düren, Germany) or the PureYield Plasmid Midiprep system (Promega, Mannheim, Germany) was employed. .. Plasmid DNA from Campylobacter cells was isolated with the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany).

    Article Title: Fluorescence in situ hybridization and qPCR to detect Merkel cell polyomavirus physical status and load in Merkel cell carcinomas.
    Article Snippet: MCPyV DNA was isolated from a MCC and cloned into a StrataClone PCR Cloning Vector (pSC-A-amp/kan; Stratagene, Santa Clara, CA). .. The plasmid DNA was purified with the plasmid DNA Purification Kit NucleoBondVR PC 2000 (Macherey-Nagel, Dueren, Germany) and sequenced using T7 and T3 primers. .. The HPyV-6 and -7 probes were generated from the 24727 plasmid: pHPyV6-607a and 24728 plasmid: pHPyV7-13a, respectively (Addgene, Cambridge, MA).

    Article Title: Development of Experimental Genetic Tools for Campylobacter fetus
    Article Snippet: .. Plasmids were purified routinely from E. coli by alkaline lysis (3); for large-scale preparations, the NucleoBond PC 2000 kit (Macherey-Nagel, Düren, Germany) or the PureYield Plasmid Midiprep system (Promega, Mannheim, Germany) was employed. .. Plasmid DNA from Campylobacter cells was isolated with the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany).

    Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles
    Article Snippet: .. In short, the plasmid pMET7-gag-EGFP was purified from DH10B E. coli via the PC2000 nucleobond kit (Cat. No: MN 740576, Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions. .. For rEV generation, HEK293T cells were seeded in Falcon cell culture Multi-Flasks (Cat. No: 353144, Corning incorporated, Corning, NY, USA) and transiently transfected at 70–80% confluency using 25 kDa linear polyethyleneimine (PEI) (Cat. No: 23966, Polysciences, Niles, IL, USA) in a PEI:DNA ratio of 5:1 with a final concentration of 1 μg DNA/mL culture medium in a total volume of 120 mL per Multi-Flask.

    Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles.
    Article Snippet: .. In short, the plasmid pMET7-gag-EGFP was purified from DH10B E. coli via the PC2000 nucleobond kit (Cat. No: MN 740576, Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions. .. For rEV generation, HEK293T cells were seeded in Falcon cell culture Multi-Flasks (Cat. No: 353144, Corning incorporated, Corning, NY, USA) and transiently transfected at 70–80% confluency using 25 kDa linear polyethyleneimine (PEI) (Cat. No: 23966, Polysciences, Niles, IL, USA) in a PEI:DNA ratio of 5:1 with a final concentration of 1 μg DNA/mL culture medium in a total volume of 120 mL per Multi-Flask.

    Isolation:

    Article Title: The role of transposon activity in shaping cis-regulatory element evolution after whole genome duplication
    Article Snippet: Reporter plasmid libraries were made by cloning amplified ATAC fragments into AgeI-HF- and SalI-HF-linearized pSTARR-seq plasmid using InFusion HD cloning kit (Takara) and then propagated in MegaX DH10B T1R electrocompetent bacteria. .. Plasmids were isolated using the NucleoBond® PC 2000 Mega kit (MACHEREY-NAGEL). ..

    Article Title: The role of transposon activity in shaping cis -regulatory element evolution after whole genome duplication.
    Article Snippet: Whole-genome duplications (WGD) and transposable element (TE) activity can act synergistically in genome evolution.. WGDs can increase TE activity directly through cellular stress or indirectly by relaxing selection against TE insertions in functionally redundant, duplicated regions.. Because TEs can function as, or evolve into, TE-derived cis-regulatory elements (TE-CREs), bursts of TE activity following WGD are therefore likely to impact evolution of gene regulation.

    DNA Purification:

    Article Title: Fluorescence in situ hybridization and qPCR to detect Merkel cell polyomavirus physical status and load in Merkel cell carcinomas.
    Article Snippet: MCPyV DNA was isolated from a MCC and cloned into a StrataClone PCR Cloning Vector (pSC-A-amp/kan; Stratagene, Santa Clara, CA). .. The plasmid DNA was purified with the plasmid DNA Purification Kit NucleoBondVR PC 2000 (Macherey-Nagel, Dueren, Germany) and sequenced using T7 and T3 primers. .. The HPyV-6 and -7 probes were generated from the 24727 plasmid: pHPyV6-607a and 24728 plasmid: pHPyV7-13a, respectively (Addgene, Cambridge, MA).



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