Journal: Human Reproduction (Oxford, England)

Article Title: Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation

doi: 10.1093/humrep/deab032

Figure Lengend Snippet: Podocalyxin (PCX) overexpression in Ishikawa cells. PCX was stably overexpressed (PCX-OE) in Ishikawa cells and compared with control (CON) cells. (A) Real-time RT-PCR analysis of PCX mRNA in CON and PCX-OE cells. Data normalized to 18S rRNA and expressed as fold change relative to CON (n = 4, *P < 0.05). (B) Representative western blot analysis of PCX protein, β-actin used as loading control. (C) Representative immunofluorescence analysis of PCX, F-actin and polarity marker scribble in control and PCX-OE cells. Inserts, negative controls. Scale bars, 20 µm.

Article Snippet: Stable overexpression of PCX in Ishikawa cells An expression construct of human PCX open reading frame (RC210816) and the empty pCMV6 vector (control plasmid) were purchased from Origene (Rockville, USA).

Techniques: Over Expression, Stable Transfection, Control, Quantitative RT-PCR, Western Blot, Immunofluorescence, Marker

Journal: Human Reproduction (Oxford, England)

Article Title: Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation

doi: 10.1093/humrep/deab032

Figure Lengend Snippet: Podocalyxin (PCX) inhibition of primary human trophoblast spheroid attachment. Equal numbers of trophoblast spheroids were co-cultured on top of control (CON) or PCX-overexpressing (PCX-OE) Ishikawa monolayer, and the percentage of spheroid attachment (number of attached/added) was examined over 24 h. Data expressed as median with interquartile range (n = 3-5). *P < 0.05 and **P < 0.01.

Article Snippet: Stable overexpression of PCX in Ishikawa cells An expression construct of human PCX open reading frame (RC210816) and the empty pCMV6 vector (control plasmid) were purchased from Origene (Rockville, USA).

Techniques: Inhibition, Cell Culture, Control

Journal: Human Reproduction (Oxford, England)

Article Title: Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation

doi: 10.1093/humrep/deab032

Figure Lengend Snippet: Podocalyxin (PCX) inhibition of primary human trophoblast spheroid invasion. Trophoblast spheroid invasion through control (CON) or PCX-overexpressing (PCX-OE) Ishikawa monolayer was examined at 24 h and 48 h. (A) Representative confocal images of side- and bottom-views of trophoblast spheroids (green) and Ishikawa monolayer (red). (B) Quantification of spheroid invasion. The volume of spheroid present beneath the Ishikawa monolayer was quantified by surface mapping. Data expressed as median with interquartile range (n = 4 for 24 h, n = 6 for 48 h). *P < 0.05.

Article Snippet: Stable overexpression of PCX in Ishikawa cells An expression construct of human PCX open reading frame (RC210816) and the empty pCMV6 vector (control plasmid) were purchased from Origene (Rockville, USA).

Techniques: Inhibition, Control