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pbluescript sk(−) phagemid vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript sk(−) phagemid vector

    Pbluescript Sk(−) Phagemid Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk++phagemid+vector/pmc10758284-251-1-5
    Average 90 stars, based on 1 article reviews
    pbluescript sk(−) phagemid vector - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "DNA cleavage assay for the identification of topoisomerase I inhibitors"

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors

    Journal: Nature protocols

    doi: 10.1038/nprot.2008.174


    Figure Legend Snippet:

    Techniques Used:

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Transgenic mouse model for imaging of ATF4 translational activation-related cellular stress responses in vivo
    Article Snippet: .. The PCR product was inserted into the HindIII/BamHI sites of pBluescript II SK(–) (#212206; Agilent Technologies, Santa Clara, CA), and sequenced by using universal M13-47 primer. ..

    Article Title: Simultaneous site-specific integrations of multiple gene-copies
    Article Snippet: Purchased Material (E. coli, Plasmid and Kits) E. coli DH5-alpha (Toyobo) is used for plasmid construction and amplification. .. The commercial plasmids/vectors TOPO cloning kit (Invitrogen) and pBluescript II SK- (Stratagene #212206) are used for cloning of PCR fragments. .. Amplified plasmids are recovered with Qiagen® Plasmid Kit (Qiagen).

    Article Title: Modified filamentous fungal host cell for encoding a secreted polypetide of interest
    Article Snippet: E. coli DH5-alpha (Toyobo) was used for plasmid construction and amplification. .. The commercial plasmid pBluescript II SK- (Stratagene #212206) was used for cloning of PCR fragments. .. Amplified plasmids were recovered with QiagenTM Plasmid Kit (Qiagen).

    other:

    Article Title: IL-12-induced T-bet expression and IFNgamma release in lymphocytes from asthmatics--role of MAPkinases ERK-1/-2, p38(MAPK) and effect of dexamethasone.
    Article Snippet: A backbone pBluescript SK-plasmid (Stratagene, La Jolla, CA, USA, cat# 212206) was linearized with BamHI and dephosphorilized.

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors
    Article Snippet: REAGENTS pBluescript SK(−) Phagemid Vector (Stratagene, cat. no. 212206).

    Article Title: Probing essential oil biosynthesis and secretion by functional evaluation of expressed sequence tags from mint glandular trichomes.
    Article Snippet: Low-level expression, without subcloning, was achieved from pBluescript SK(2) (Stratagene, no. 212206) under control of the lac promoter (E. coli SOLR cells as a host, Stratagene, no. 200298), which was induced by addition of 1.0–2.5 mM isopropyl-1-thiob-D-galactopyranoside (IPTG).

    Cloning:

    Article Title: Simultaneous site-specific integrations of multiple gene-copies
    Article Snippet: Purchased Material (E. coli, Plasmid and Kits) E. coli DH5-alpha (Toyobo) is used for plasmid construction and amplification. .. The commercial plasmids/vectors TOPO cloning kit (Invitrogen) and pBluescript II SK- (Stratagene #212206) are used for cloning of PCR fragments. .. Amplified plasmids are recovered with Qiagen® Plasmid Kit (Qiagen).

    Article Title: Modified filamentous fungal host cell for encoding a secreted polypetide of interest
    Article Snippet: E. coli DH5-alpha (Toyobo) was used for plasmid construction and amplification. .. The commercial plasmid pBluescript II SK- (Stratagene #212206) was used for cloning of PCR fragments. .. Amplified plasmids were recovered with QiagenTM Plasmid Kit (Qiagen).

    Article Title: The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides gRNA targeting sequence for MRLC: UUACUGCUC AUCCUGUCCU this paper N/A Oligonucleotides for generating MRLC::SuperNova knockin flies, see Table S1 this paper N/A Primers for cloning MRLC, see Table S1 this paper N/A Primers for cloning the intracellular domain of Sdk, see Table S1 this paper N/A Primers for cloning GFP, see Table S1 this paper N/A Primers for cloning Ruby, see Table S1 this paper N/A Recombinant DNA pBFv-U6.2 National Institute of Genetics NIG: pBFv-U6.2 pBluescript SK(–) Agilent Cat# 212206 pWAGAL4 (pAct5C-Gal4) Yasushi Hiromi (National Institute of Genetics) FBal0097155 pUAST Brand and Perrimon, 1993 N/A pUAST-Flag this paper N/A pUAST-Flag-sdkICD this paper N/A pUAST-Venus this paper N/A pUAST-Venus-sdkICD this paper N/A pUAST-MRLC-Venus this paper N/A pUAST-b-Catenin Kanuka et al., 2005 N/A pUAST-HA-sdk Astigarraga et al., 2018 N/A pCaspR-Ubi-E-Cad-GFP Oda and Tsukita, 2001 N/A pUAST-HA-sdk-GFP this paper N/A pUAST-HA-sdk-Ruby this paper N/A Software and Algorithms R The R foundation RRID: SCR_001905; https://www. r-project.org/ Fiji ImageJ, NIH RRID: SCR_002285; https://fiji.sc/ ..

    Plasmid Preparation:

    Article Title: Modified filamentous fungal host cell for encoding a secreted polypetide of interest
    Article Snippet: E. coli DH5-alpha (Toyobo) was used for plasmid construction and amplification. .. The commercial plasmid pBluescript II SK- (Stratagene #212206) was used for cloning of PCR fragments. .. Amplified plasmids were recovered with QiagenTM Plasmid Kit (Qiagen).

    Sequencing:

    Article Title: The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides gRNA targeting sequence for MRLC: UUACUGCUC AUCCUGUCCU this paper N/A Oligonucleotides for generating MRLC::SuperNova knockin flies, see Table S1 this paper N/A Primers for cloning MRLC, see Table S1 this paper N/A Primers for cloning the intracellular domain of Sdk, see Table S1 this paper N/A Primers for cloning GFP, see Table S1 this paper N/A Primers for cloning Ruby, see Table S1 this paper N/A Recombinant DNA pBFv-U6.2 National Institute of Genetics NIG: pBFv-U6.2 pBluescript SK(–) Agilent Cat# 212206 pWAGAL4 (pAct5C-Gal4) Yasushi Hiromi (National Institute of Genetics) FBal0097155 pUAST Brand and Perrimon, 1993 N/A pUAST-Flag this paper N/A pUAST-Flag-sdkICD this paper N/A pUAST-Venus this paper N/A pUAST-Venus-sdkICD this paper N/A pUAST-MRLC-Venus this paper N/A pUAST-b-Catenin Kanuka et al., 2005 N/A pUAST-HA-sdk Astigarraga et al., 2018 N/A pCaspR-Ubi-E-Cad-GFP Oda and Tsukita, 2001 N/A pUAST-HA-sdk-GFP this paper N/A pUAST-HA-sdk-Ruby this paper N/A Software and Algorithms R The R foundation RRID: SCR_001905; https://www. r-project.org/ Fiji ImageJ, NIH RRID: SCR_002285; https://fiji.sc/ ..

    Knock-In:

    Article Title: The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides gRNA targeting sequence for MRLC: UUACUGCUC AUCCUGUCCU this paper N/A Oligonucleotides for generating MRLC::SuperNova knockin flies, see Table S1 this paper N/A Primers for cloning MRLC, see Table S1 this paper N/A Primers for cloning the intracellular domain of Sdk, see Table S1 this paper N/A Primers for cloning GFP, see Table S1 this paper N/A Primers for cloning Ruby, see Table S1 this paper N/A Recombinant DNA pBFv-U6.2 National Institute of Genetics NIG: pBFv-U6.2 pBluescript SK(–) Agilent Cat# 212206 pWAGAL4 (pAct5C-Gal4) Yasushi Hiromi (National Institute of Genetics) FBal0097155 pUAST Brand and Perrimon, 1993 N/A pUAST-Flag this paper N/A pUAST-Flag-sdkICD this paper N/A pUAST-Venus this paper N/A pUAST-Venus-sdkICD this paper N/A pUAST-MRLC-Venus this paper N/A pUAST-b-Catenin Kanuka et al., 2005 N/A pUAST-HA-sdk Astigarraga et al., 2018 N/A pCaspR-Ubi-E-Cad-GFP Oda and Tsukita, 2001 N/A pUAST-HA-sdk-GFP this paper N/A pUAST-HA-sdk-Ruby this paper N/A Software and Algorithms R The R foundation RRID: SCR_001905; https://www. r-project.org/ Fiji ImageJ, NIH RRID: SCR_002285; https://fiji.sc/ ..

    Recombinant:

    Article Title: The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides gRNA targeting sequence for MRLC: UUACUGCUC AUCCUGUCCU this paper N/A Oligonucleotides for generating MRLC::SuperNova knockin flies, see Table S1 this paper N/A Primers for cloning MRLC, see Table S1 this paper N/A Primers for cloning the intracellular domain of Sdk, see Table S1 this paper N/A Primers for cloning GFP, see Table S1 this paper N/A Primers for cloning Ruby, see Table S1 this paper N/A Recombinant DNA pBFv-U6.2 National Institute of Genetics NIG: pBFv-U6.2 pBluescript SK(–) Agilent Cat# 212206 pWAGAL4 (pAct5C-Gal4) Yasushi Hiromi (National Institute of Genetics) FBal0097155 pUAST Brand and Perrimon, 1993 N/A pUAST-Flag this paper N/A pUAST-Flag-sdkICD this paper N/A pUAST-Venus this paper N/A pUAST-Venus-sdkICD this paper N/A pUAST-MRLC-Venus this paper N/A pUAST-b-Catenin Kanuka et al., 2005 N/A pUAST-HA-sdk Astigarraga et al., 2018 N/A pCaspR-Ubi-E-Cad-GFP Oda and Tsukita, 2001 N/A pUAST-HA-sdk-GFP this paper N/A pUAST-HA-sdk-Ruby this paper N/A Software and Algorithms R The R foundation RRID: SCR_001905; https://www. r-project.org/ Fiji ImageJ, NIH RRID: SCR_002285; https://fiji.sc/ ..

    Software:

    Article Title: The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides gRNA targeting sequence for MRLC: UUACUGCUC AUCCUGUCCU this paper N/A Oligonucleotides for generating MRLC::SuperNova knockin flies, see Table S1 this paper N/A Primers for cloning MRLC, see Table S1 this paper N/A Primers for cloning the intracellular domain of Sdk, see Table S1 this paper N/A Primers for cloning GFP, see Table S1 this paper N/A Primers for cloning Ruby, see Table S1 this paper N/A Recombinant DNA pBFv-U6.2 National Institute of Genetics NIG: pBFv-U6.2 pBluescript SK(–) Agilent Cat# 212206 pWAGAL4 (pAct5C-Gal4) Yasushi Hiromi (National Institute of Genetics) FBal0097155 pUAST Brand and Perrimon, 1993 N/A pUAST-Flag this paper N/A pUAST-Flag-sdkICD this paper N/A pUAST-Venus this paper N/A pUAST-Venus-sdkICD this paper N/A pUAST-MRLC-Venus this paper N/A pUAST-b-Catenin Kanuka et al., 2005 N/A pUAST-HA-sdk Astigarraga et al., 2018 N/A pCaspR-Ubi-E-Cad-GFP Oda and Tsukita, 2001 N/A pUAST-HA-sdk-GFP this paper N/A pUAST-HA-sdk-Ruby this paper N/A Software and Algorithms R The R foundation RRID: SCR_001905; https://www. r-project.org/ Fiji ImageJ, NIH RRID: SCR_002285; https://fiji.sc/ ..



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    Image Search Results


    Journal: Nature protocols

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors

    doi: 10.1038/nprot.2008.174

    Figure Lengend Snippet:

    Article Snippet: REAGENTS pBluescript SK(−) Phagemid Vector (Stratagene, cat. no. 212206).

    Techniques:

    cDNA Library Attributes

    Journal: BMC Plant Biology

    Article Title: Identification of tissue-specific, abiotic stress-responsive gene expression patterns in wine grape ( Vitis vinifera L.) based on curation and mining of large-scale EST data sets

    doi: 10.1186/1471-2229-11-86

    Figure Lengend Snippet: cDNA Library Attributes

    Article Snippet: The cDNAs were then directionally cloned into EcoRI-NotI digested pBluescript II SK+ phagemid vector (Stratagene, Inc., La Jolla, CA).

    Techniques: cDNA Library Assay, Clone Assay, Sequencing, Plasmid Preparation