pbluescript ii ks(+) phagemid vector (Agilent technologies)
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pbluescript ii ks(+) phagemid vector
Pbluescript Ii Ks(+) Phagemid Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+phagemid+ks++vector/pmc05508591-571-13-18
Average 90 stars, based on 1 article reviews
Pbluescript Ii Ks(+) Phagemid Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+phagemid+ks++vector/pmc05508591-571-13-18
Average 90 stars, based on 1 article reviews
pbluescript ii ks(+) phagemid vector - by Bioz Stars,
2026-11
90/100 stars
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Polymerase Chain Reaction:Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions. Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Clone Assay:Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions. Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Article Title: TBX5 drives Aldh1a2 expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development Article Snippet: Xenopus transgenic plasmids were constructed using the pI- SceI-d2EGFP plasmid backbone (Addgene 32674). .. First, a fragment containing the mouse or X.trop enh1 enhancers upstream of a minimal TATA box promoter ( ) flanked by duplicated copies of the 250bp chick B-globin HS4 insulator ( ; ) was commercially synthesized (Genscript USA) and cloned into the ApaI/XhoI sites of Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. Generation of UAS constructs and transgenic lines The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Plasmid Preparation:Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions. Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Article Title: Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI (4’, 6-diamidino-2-phenylindole) Invitrogen Molecular Probes Monoclonal Rabbit Anti Dbx1 Pierani et al., 2001 N/A Polyclonal Rabbit Anti Pax 6 Proteintech #12323-1-AP Polyclonal Rabbit Anti GABA Sigma #A2052 Monoclonal Mouse Anti GABA Sigma #A2082 Polyclonal Rabbit Tbr1 Abcam #ab31940 Polyclonal Rabbit Tbr2 AbCys S.A #VPA9618 Polyclonal Rabbit Cre recombinase Covance #PRB-106C Polyclonal Rabbit Calretinin for mouse Sawant #7699/3H Polyclonal Rabbit Calretinin for human Sawant #7699/4 Monoclonal Mouse Calretinin for macaque Sawant #6B3 Monoclonal Mouse Reln clone G10 Millipore #MAB5364 Monoclonal Mouse Reln clone 142 Milipore #MAB5366 Monoclonal Mouse Tuj1 Bio Legend #MMS-435P Polyclonal Goat Nurr1 R&D systems #AF2156 Monoclonal Rat Ctip2 Abcam #ab18465 Polyclonal Chicken Tbr2 Millipore #AB15894 Polyclonal Chicken GFP Aves labs #GFP-1020 Monoclonal Rat Neural cell adhesion molecule L1 Millipore #MAB5272 Biological Samples Macaca mulatta embryos Lukaszewicz et al., 2005 N/A Human embryos GW 6.5 Maternity and Fetopathology departments, Robert Debre Hospital, Paris N/A Critical Commercial Assays mMESSAGE mMACHINE kit Life Technologies #AM1344 DIG-RNA labeling kit Roche # 11277073910 Experimental Models: Organisms/Strains C57BL/6J pregnant females Janvier Labs C57BL/6J NexCre embryos Goebbels et al., 2006 N/A Oligonucleotides Antisense RNA probes for Dbx1 Pierani et al., 2001 N/A Marmosets DNAs (template) Drs. Shiozawa and Imamura from H. Okano laboratory N/A Primer: marmoset 10kb-EGFP 50-CTCCTTACAATT TTATTTACAGTCAAAAAG-30 (forward) This paper N/A Primer: marmoset 10kb-EGFP 50-GTGACCAGCCT GTATTTAGATACAT-30 (reverse) This paper N/A Primer: marmoset 10kb-EGFP 50-TTCTAGTTGCCA GCCATCTGTTGTT-30 (forward) This paper N/A Primer: marmoset 10kb-EGFP 50-TCCATTAAAATT GTACTTGAGTATTAGATC-30 (reverse) This paper N/A Recombinant DNA pCAGGS-ires-EGFP Addgene #32482 pCAGGS-HA-mouseDbx-ires-EGFP This paper N/A (Continued on next page) Cell Reports 29, 645–658.e1–e5, October 15, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCAGGS-loxP-stop-loxP-EGFP gift from Dr. Fei N/A pCAGGS-loxP-stop-loxP-HA-mouseDbx-ires-EGFP This paper N/A pCAGGS-ires-NLS-EGFP vector Karaz et al., 2016 N/A pCAGGS-HA-mouseDbx1-ires-NLS-EGFP This paper N/A HA-mouseDbx1-ires-NLS-EGFP This paper N/A pCAGGS-mcherry Addgene #41583 mouse 5kb-EGFP This paper N/A marmoset 5kb-EGFP This paper N/A marmoset 10kb-EGFP This paper N/A pCS-TP plasmid Sumiyama et al., Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Generated:Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions. Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Article Title: Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions Article Snippet: .. PCR products were cloned into BamHI and SalI sites of Software:Article Title: Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI (4’, 6-diamidino-2-phenylindole) Invitrogen Molecular Probes Monoclonal Rabbit Anti Dbx1 Pierani et al., 2001 N/A Polyclonal Rabbit Anti Pax 6 Proteintech #12323-1-AP Polyclonal Rabbit Anti GABA Sigma #A2052 Monoclonal Mouse Anti GABA Sigma #A2082 Polyclonal Rabbit Tbr1 Abcam #ab31940 Polyclonal Rabbit Tbr2 AbCys S.A #VPA9618 Polyclonal Rabbit Cre recombinase Covance #PRB-106C Polyclonal Rabbit Calretinin for mouse Sawant #7699/3H Polyclonal Rabbit Calretinin for human Sawant #7699/4 Monoclonal Mouse Calretinin for macaque Sawant #6B3 Monoclonal Mouse Reln clone G10 Millipore #MAB5364 Monoclonal Mouse Reln clone 142 Milipore #MAB5366 Monoclonal Mouse Tuj1 Bio Legend #MMS-435P Polyclonal Goat Nurr1 R&D systems #AF2156 Monoclonal Rat Ctip2 Abcam #ab18465 Polyclonal Chicken Tbr2 Millipore #AB15894 Polyclonal Chicken GFP Aves labs #GFP-1020 Monoclonal Rat Neural cell adhesion molecule L1 Millipore #MAB5272 Biological Samples Macaca mulatta embryos Lukaszewicz et al., 2005 N/A Human embryos GW 6.5 Maternity and Fetopathology departments, Robert Debre Hospital, Paris N/A Critical Commercial Assays mMESSAGE mMACHINE kit Life Technologies #AM1344 DIG-RNA labeling kit Roche # 11277073910 Experimental Models: Organisms/Strains C57BL/6J pregnant females Janvier Labs C57BL/6J NexCre embryos Goebbels et al., 2006 N/A Oligonucleotides Antisense RNA probes for Dbx1 Pierani et al., 2001 N/A Marmosets DNAs (template) Drs. Shiozawa and Imamura from H. Okano laboratory N/A Primer: marmoset 10kb-EGFP 50-CTCCTTACAATT TTATTTACAGTCAAAAAG-30 (forward) This paper N/A Primer: marmoset 10kb-EGFP 50-GTGACCAGCCT GTATTTAGATACAT-30 (reverse) This paper N/A Primer: marmoset 10kb-EGFP 50-TTCTAGTTGCCA GCCATCTGTTGTT-30 (forward) This paper N/A Primer: marmoset 10kb-EGFP 50-TCCATTAAAATT GTACTTGAGTATTAGATC-30 (reverse) This paper N/A Recombinant DNA pCAGGS-ires-EGFP Addgene #32482 pCAGGS-HA-mouseDbx-ires-EGFP This paper N/A (Continued on next page) Cell Reports 29, 645–658.e1–e5, October 15, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCAGGS-loxP-stop-loxP-EGFP gift from Dr. Fei N/A pCAGGS-loxP-stop-loxP-HA-mouseDbx-ires-EGFP This paper N/A pCAGGS-ires-NLS-EGFP vector Karaz et al., 2016 N/A pCAGGS-HA-mouseDbx1-ires-NLS-EGFP This paper N/A HA-mouseDbx1-ires-NLS-EGFP This paper N/A pCAGGS-mcherry Addgene #41583 mouse 5kb-EGFP This paper N/A marmoset 5kb-EGFP This paper N/A marmoset 10kb-EGFP This paper N/A pCS-TP plasmid Sumiyama et al., other:Article Title: Synthetic DNA vectors and methods of use Article Snippet: This DNA is then digested with an enzyme (EcoRI) that cuts once within the rAAV genome to produce a unit-length genome that is cloned into Synthesized:Article Title: TBX5 drives Aldh1a2 expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development Article Snippet: Xenopus transgenic plasmids were constructed using the pI- SceI-d2EGFP plasmid backbone (Addgene 32674). .. First, a fragment containing the mouse or X.trop enh1 enhancers upstream of a minimal TATA box promoter ( ) flanked by duplicated copies of the 250bp chick B-globin HS4 insulator ( ; ) was commercially synthesized (Genscript USA) and cloned into the ApaI/XhoI sites of Construct:Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. Generation of UAS constructs and transgenic lines The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Transgenic Assay:Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. Generation of UAS constructs and transgenic lines The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Amplification:Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. Generation of UAS constructs and transgenic lines The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Reverse Transcription Polymerase Chain Reaction:Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. Generation of UAS constructs and transgenic lines The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of Article Title: CCB is Involved in Actin-Based Axonal Transport of Selected Synaptic Proteins Article Snippet: .. The open reading frame (ORF) of CG14579 , the putative ccb gene, was amplified by RT-PCR from Canton-S wild-type mRNA using the forward primer 5′-CCATATATCAGCGGCCGCATTTGAAAATGAACAGCAGGTG-3′ and the reverse primer 5′-GCGGTACCGGTTTGGCCAGTCCTCA-3′ and cloned into Kpn-I and Not-I restriction sites of |