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pbluescript ii ks(−) phagemid vector  (Agilent technologies)


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    Agilent technologies pbluescript ii ks(−) phagemid vector
    Pbluescript Ii Ks(−) Phagemid Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+phagemid+ks++vector/pmc01072808-22-9-14
    Average 90 stars, based on 1 article reviews
    pbluescript ii ks(−) phagemid vector - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.
    Article Snippet: Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago.. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here.. Amplification and polymorphism levels appeared to be high in these Hawaiian species.

    Article Title: Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae).
    Article Snippet: Galium catalinense subspecies acrispum (Rubiaceae) is a state-endangered perennial shrub endemic to San Clemente Island.. Eight polymorphic microsatellite loci were isolated from G. catalinense ssp. acrispum.. These loci show high levels of variability, averaging 6.5 alleles per locus and an expected heterozygosity of 0.550.

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Article Title: Production of a mouse strain with impaired glucose tolerance by systemic heterozygous knockout of the glucokinase gene and its feasibility as a prediabetes model
    Article Snippet: Two parts of a long arm ((1) and (2)), a short arm, and 2 cassettes (DT-A and neo) were inserted into a multiple cloning site of a pBluescript® II SK (+) Phagemid Vector (Agilent Technologies, Santa Clara, CA, USA) according to the protocol illustrated in .

    Article Title: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme
    Article Snippet: Deletion of H. suis ggt was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~650 bp of the 5′ –end and ~750 bp of the 3′ –end of the target gene and the chloramphenicol resistance gene from pUOA14 [ , ] were ligated through a PCR-mediated strategy [ , ].

    Plasmid Preparation:

    Article Title: Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.
    Article Snippet: Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago.. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here.. Amplification and polymorphism levels appeared to be high in these Hawaiian species.

    Article Title: Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae).
    Article Snippet: Galium catalinense subspecies acrispum (Rubiaceae) is a state-endangered perennial shrub endemic to San Clemente Island.. Eight polymorphic microsatellite loci were isolated from G. catalinense ssp. acrispum.. These loci show high levels of variability, averaging 6.5 alleles per locus and an expected heterozygosity of 0.550.

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Article Title: Production of a mouse strain with impaired glucose tolerance by systemic heterozygous knockout of the glucokinase gene and its feasibility as a prediabetes model
    Article Snippet: Two parts of a long arm ((1) and (2)), a short arm, and 2 cassettes (DT-A and neo) were inserted into a multiple cloning site of a pBluescript® II SK (+) Phagemid Vector (Agilent Technologies, Santa Clara, CA, USA) according to the protocol illustrated in .

    Article Title: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme
    Article Snippet: Deletion of H. suis ggt was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~650 bp of the 5′ –end and ~750 bp of the 3′ –end of the target gene and the chloramphenicol resistance gene from pUOA14 [ , ] were ligated through a PCR-mediated strategy [ , ].

    Polymerase Chain Reaction:

    Article Title: Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.
    Article Snippet: Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago.. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here.. Amplification and polymorphism levels appeared to be high in these Hawaiian species.

    Article Title: Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae).
    Article Snippet: Galium catalinense subspecies acrispum (Rubiaceae) is a state-endangered perennial shrub endemic to San Clemente Island.. Eight polymorphic microsatellite loci were isolated from G. catalinense ssp. acrispum.. These loci show high levels of variability, averaging 6.5 alleles per locus and an expected heterozygosity of 0.550.

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Article Title: Production of a mouse strain with impaired glucose tolerance by systemic heterozygous knockout of the glucokinase gene and its feasibility as a prediabetes model
    Article Snippet: Two parts of a long arm ((1) and (2)), a short arm, and 2 cassettes (DT-A and neo) were inserted into a multiple cloning site of a pBluescript® II SK (+) Phagemid Vector (Agilent Technologies, Santa Clara, CA, USA) according to the protocol illustrated in .

    Article Title: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme
    Article Snippet: Deletion of H. suis ggt was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~650 bp of the 5′ –end and ~750 bp of the 3′ –end of the target gene and the chloramphenicol resistance gene from pUOA14 [ , ] were ligated through a PCR-mediated strategy [ , ].

    Purification:

    Article Title: Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.
    Article Snippet: Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago.. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here.. Amplification and polymorphism levels appeared to be high in these Hawaiian species.

    Article Title: Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae).
    Article Snippet: Galium catalinense subspecies acrispum (Rubiaceae) is a state-endangered perennial shrub endemic to San Clemente Island.. Eight polymorphic microsatellite loci were isolated from G. catalinense ssp. acrispum.. These loci show high levels of variability, averaging 6.5 alleles per locus and an expected heterozygosity of 0.550.

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Article Title: Production of a mouse strain with impaired glucose tolerance by systemic heterozygous knockout of the glucokinase gene and its feasibility as a prediabetes model
    Article Snippet: Two parts of a long arm ((1) and (2)), a short arm, and 2 cassettes (DT-A and neo) were inserted into a multiple cloning site of a pBluescript® II SK (+) Phagemid Vector (Agilent Technologies, Santa Clara, CA, USA) according to the protocol illustrated in .

    Article Title: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme
    Article Snippet: Deletion of H. suis ggt was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~650 bp of the 5′ –end and ~750 bp of the 3′ –end of the target gene and the chloramphenicol resistance gene from pUOA14 [ , ] were ligated through a PCR-mediated strategy [ , ].

    Ligation:

    Article Title: Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.
    Article Snippet: Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago.. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here.. Amplification and polymorphism levels appeared to be high in these Hawaiian species.

    Article Title: Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae).
    Article Snippet: Galium catalinense subspecies acrispum (Rubiaceae) is a state-endangered perennial shrub endemic to San Clemente Island.. Eight polymorphic microsatellite loci were isolated from G. catalinense ssp. acrispum.. These loci show high levels of variability, averaging 6.5 alleles per locus and an expected heterozygosity of 0.550.

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Article Title: Production of a mouse strain with impaired glucose tolerance by systemic heterozygous knockout of the glucokinase gene and its feasibility as a prediabetes model
    Article Snippet: Two parts of a long arm ((1) and (2)), a short arm, and 2 cassettes (DT-A and neo) were inserted into a multiple cloning site of a pBluescript® II SK (+) Phagemid Vector (Agilent Technologies, Santa Clara, CA, USA) according to the protocol illustrated in .

    Article Title: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme
    Article Snippet: Deletion of H. suis ggt was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~650 bp of the 5′ –end and ~750 bp of the 3′ –end of the target gene and the chloramphenicol resistance gene from pUOA14 [ , ] were ligated through a PCR-mediated strategy [ , ].

    Inverse PCR:

    Article Title: Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.
    Article Snippet: Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago.. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here.. Amplification and polymorphism levels appeared to be high in these Hawaiian species.

    Article Title: Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae).
    Article Snippet: Galium catalinense subspecies acrispum (Rubiaceae) is a state-endangered perennial shrub endemic to San Clemente Island.. Eight polymorphic microsatellite loci were isolated from G. catalinense ssp. acrispum.. These loci show high levels of variability, averaging 6.5 alleles per locus and an expected heterozygosity of 0.550.

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Article Title: Production of a mouse strain with impaired glucose tolerance by systemic heterozygous knockout of the glucokinase gene and its feasibility as a prediabetes model
    Article Snippet: Two parts of a long arm ((1) and (2)), a short arm, and 2 cassettes (DT-A and neo) were inserted into a multiple cloning site of a pBluescript® II SK (+) Phagemid Vector (Agilent Technologies, Santa Clara, CA, USA) according to the protocol illustrated in .

    Article Title: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme
    Article Snippet: Deletion of H. suis ggt was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~650 bp of the 5′ –end and ~750 bp of the 3′ –end of the target gene and the chloramphenicol resistance gene from pUOA14 [ , ] were ligated through a PCR-mediated strategy [ , ].



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