pbluescript ii ks (Promega)

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Promega pbluescript ii ks
Pbluescript Ii Ks, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+ii+phagemid+ks++vector/pbluescript/pm23929524-53-38-42
Average 90 stars, based on 1 article reviews

pbluescript ii ks - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Acquisition of neural fate by combination of BMP blockade and chromatin modification
Article Snippet: pBluescript -SK , Promega , N/A.

Article Title: How novel methods can help discover more information about foodborne pathogens
Article Snippet: A Luciola mingrelica luc recombinant AR1 has been obtained by simultaneously infecting wild type E coli O157:H7 with a pBluescript II SK (±) phagemid containing the luc gene (Promega Corporation, Madison, Wisconsin) and AR1 ( 105 ).

Article Title: Coelimycin inside out — negative feedback regulation by its intracellular precursors
Article Snippet: Plasmid/cosmid Description Source or reference St1G7 SuperCos1 cosmid carrying fragment of S. coelicolor A3(2) chromosome encompassing part of cpk gene cluster including cpkF gene (bp 6905834 to 6947687) http://www.strepdb. streptomyces.org.uk St1G7-cpkFDM St1G7 cosmid derivative, in which cpkF gene was replaced by means of PCR-targeting with a hygromycin resistance cassette hyg amplified with primers CpkF_D_Fw and CpkF_D_Rv on the template of pIJ10700 plasmid linearized by digestion with XhoI; recombineering was performed in E.coli BW25113/pIJ790 this work pIJ10700 pBluescript II KS(+) containing hyg-oriT cassette (Gust et al. 2004) pGEM-T Easy T-vector for direct cloning of PCR products (AmpR) Promega pGEM-cpkF pGEM-T Easy with cpkF gene amplified with primers CpkF_Ex_Fw and CpkF_Ex_W this work pET-21b(+) Expression vector for His-tagged recombinant proteins Novagen pWP2 pET-21b(+) plasmid with cpkF gene (NdeI-HindIII fragment from pGEM-cpkF) this work pCJW93 High copy number plasmid, ApraR (aac3(IV)), oriT (RK2), ThioR (tsr), promoter tipA (Wilkinson et al. 2002) pWP3 Plasmid pCJW93 in which PtipA promoter replaced with ermEp* (Pawlik et al. 2021) pWP4 pWP3 plasmid with cpkF gene; Bpu1102I (blunted with S1 nuclease)-NdeI fragment from pWP2 plasmid was inserted into EcoRI (blunted with S1 nuclease) and NdeI sites of pWP3 this work pGEX-6P-2 Expression vector for GST fusion proteins GE Healthcare pSS2 pGEX-6P-2 plasmid with cpkF gene amplified with primers CpkF_Ex_Fw and CpkF_Ex_R was inserted into BamHI and EcoRI sites pIJ10257 ΦBT1 integrating overexpression plasmid containing strong constitutive promoter ermEp*, HygR (Hong et al. 2005) pIJ10257-cpkF pIJ10257 plasmid with cpkF gene (NdeI-XhoI fragment from pSS2) this work pSET152 ΦC31 integrating plasmid, ApraR (aac3(IV)) (Kieser et al. 2000) pIJ10257apra pIJ10257 plasmid in which hygromycin resistance was replaced with apramycin resistance gene.

Article Title: The Functional Epididymal Amyloid Cystatin-Related Epididymal Spermatogenic (CRES) is a Component of the Mammalian Brain Extracellular Matrix
Article Snippet: PCR products for the CRES subgroup members and cystatin C were cloned using the TOPO TA cloning kit (Invitrogen, Waltham MA) except for the Cst8 gene.

Cloning:

Article Title: Mechanistic Insights into a Novel Exporter-Importer System of Mycobacterium tuberculosis Unravel Its Role in Trafficking of Iron
Article Snippet: .. pBluescript II SK(+) , Cloning vector , Promega. .. pET23a , E. coli expression vector , Novagen.

Article Title: Materials and methods for respiratory disease control in canines
Article Snippet: .. A number of vectors are available for expression and/or cloning, and include, but are not limited to, pBR322, pUC series, M13 series, pGEM series, and pBLUESCRIPT vectors (Stratagene, La Jolla, CA and Promega, Madison, WI). ..

Plasmid Preparation:

Expressing:

Amplification:

Article Title: Hybrids of RNA viruses and viroid-like elements replicate in fungi
Article Snippet: .. The cDNAs of viral RNA fragments containing the ribozymes were either amplified using the appropriate primers listed in Supplementary Data or purchased as gBlock Gene Fragments (Integrated DNA Technologies), subcloned in either pBlueScript KS or pGEM-T easy Promega, (Madison, WI, United States) and sequenced to confirm the orientation and absence of unwanted mutations. .. The recombinant plasmids, linearized with the appropriate restriction enzyme, were transcribed in vitro with T7 RNA polymerase (Thermo-Fisher Scientific) at 37 °C for 2 h using buffer provided by the manufacturer and the reaction products separated by denaturing 5% PAGE 8 M urea and 1× TBE (89 mM Tris, 89 mM boric acid, 2.5 mM EDTA, pH 8.3), stained with ethidium bromide and UV visualized.

Article Title: Hybrids of RNA viruses and viroid-like elements replicate in fungi.
Article Snippet: .. The cDNAs of viral RNA fragments containing the ribozymes were either amplified using the appropriate primers listed in Supplementary Data 1 or purchased as gBlock Gene Fragments (Integrated DNA Technologies), subcloned in either pBlueScript KS or pGEM-T easy Promega, (Madison, WI, United States) and sequenced to confirm the orientation and absence of unwanted mutations. .. The recombinant plasmids, linearized with the appropriate restriction enzyme, were transcribed in vitro with T7 RNA polymerase (Thermo-Fisher Scientific) at 37 °C for 2 h using buffer provided by the manufacturer and the reaction products separated by denaturing 5% PAGE 8M urea and 1× TBE (89mM Tris, 89mM boric acid, 2.5 mM EDTA, pH 8.3), stained with ethidium bromide and UV visualized.

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