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qiasymphony dsp virus pathogen kit  (Qiagen)


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    Structured Review

    Qiagen qiasymphony dsp virus pathogen kit
    Qiasymphony Dsp Virus Pathogen Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathogens/QIAsymphony+DSP+Virus%2FPathogen+Mini+Kit/pmc10769880-253-20-28
    Average 97 stars, based on 642 article reviews
    qiasymphony dsp virus pathogen kit - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Automated:

    Article Title: Pathogen genome enrichment sequencing to support a city-wide Legionnaires' disease outbreak investigation.
    Article Snippet: .. Genomic DNA (gDNA) was extracted from a single colony on the automated QIAsymphony platform using the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), as per the manufacturer’s instructions. .. DNA libraries were prepared for sequencing using the Nextera XT library sample preparation workflow (Illumina), and libraries were sequenced on an Illumina NextSeq 2000 instrument using 150 bp paired- end reads following instrument- specific instructions.

    Article Title: Association of alcohol consumption with oral high-risk human papillomavirus infection: a cross-sectional study within the multinational HPV infection in men (HIM) cohort
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from ORG cell pellets for HPV analysis using the QIASymphony DSP Virus/Pathogen Mini Kit on the QIASymphony SP automated extraction machine (QIAGEN, Inc.) according to the manufacturer's instructions. ..

    Virus:

    Article Title: Pathogen genome enrichment sequencing to support a city-wide Legionnaires' disease outbreak investigation.
    Article Snippet: .. Genomic DNA (gDNA) was extracted from a single colony on the automated QIAsymphony platform using the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), as per the manufacturer’s instructions. .. DNA libraries were prepared for sequencing using the Nextera XT library sample preparation workflow (Illumina), and libraries were sequenced on an Illumina NextSeq 2000 instrument using 150 bp paired- end reads following instrument- specific instructions.

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Genome sequences of hepatitis E virus genotype 1e from an outbreak in the Central African Republic, 2018.
    Article Snippet: Four serum samples collected during this study and with sufficient sample volume were used for whole-genome sequencing with a viral capture enrichment approach (Viral Surveillance Panel, Illumina, San Diego, CA, USA). .. Nucleic acids were extracted from 200 μL of serum using the QIAsymphony DSP Virus/Pathogen Mini Kit (QIAGEN, Hilden, Germany), with an elution volume of 90 μL. .. Extracts were processed with the Illumina RNA Prep with Enrichment, (L) Tagmentation kit for complementary DNA and double-stranded DNA synthesis, and library generation by on-bead tagmentation, which simultaneously fragments DNA and incorporates sequencing adapters.

    Article Title: First evidence of Tahyna virus in Central Italy and seasonal dynamics of its putative vector, Aedes caspius.
    Article Snippet: Mosquito pools were suspended in 0.9 ml of ATL buffer (Animal Tissue Lysis buffer, Qiagen) containing a 5 mm stainless steel bead. .. All samples were homogenised by Tissue Lyser II (QIAGEN, GmbH, Hilden, Germany), followed by a centrifugation at 16,000 g. A 400 μl volume of the supernatant was used for the nucleic acid extraction, using the automatic extractor QIAsymphony (DSP Virus/Pathogen Kit, Qiagen, GmbH, Hilden, Germany) according to the manufacturer’s instructions. .. Pools were first subjected to cDNA synthesis, which was carried out using 20 μl of RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific), following the manufacturer’s instructions, using the Gene AmpR PCR System 9700 (Applied Biosystems, Thermo Fisher Scientific, Waltham) with a thermal cycling profile of 10 min at 25 ◦C and 45 min at 37 ◦C.

    Article Title: Associations between commonly consumed beverages and the oral microbiome in three US cohort studies
    Article Snippet: .. Briefly, DNA was extracted from the buccal cell pellets using the DSP DNA Virus Pathogen kit (Qiagen). ..

    Article Title: Cocirculation of endemic and recently introduced West Nile Virus lineage 1 clades in Southern Spain
    Article Snippet: .. RNA was extracted using the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Hilden, Germany). ..

    Article Title: Time to improve laboratory protocols in microbial keratitis: broad-range Internal Transcribed Spacer sequencing in refractory culture-negative keratitis.
    Article Snippet: To cite: Passaro ML, Airaldi M, Somerville T, et al. BMJ Case Rep 2026;19:e274547.. doi:10.1136/bcr-2026274547 St Paul’s Eye Unit, Liverpool University Hospitals NHS Foundation Trust, Liverpool, UK Department of Medicine and Health Sciences “V.. Tiberio”, University of Molise, Campobasso, Italy Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy Department of Eye and Vision Science, University of Liverpool, Liverpool, UK St Pauls Eye Unit, NHS University Hospitals of Liverpool Group, Liverpool, UK Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK “Mons.

    Article Title: Association of alcohol consumption with oral high-risk human papillomavirus infection: a cross-sectional study within the multinational HPV infection in men (HIM) cohort
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from ORG cell pellets for HPV analysis using the QIASymphony DSP Virus/Pathogen Mini Kit on the QIASymphony SP automated extraction machine (QIAGEN, Inc.) according to the manufacturer's instructions. ..

    Clinical Proteomics:

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Purification:

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Extraction:

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Association of alcohol consumption with oral high-risk human papillomavirus infection: a cross-sectional study within the multinational HPV infection in men (HIM) cohort
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from ORG cell pellets for HPV analysis using the QIASymphony DSP Virus/Pathogen Mini Kit on the QIASymphony SP automated extraction machine (QIAGEN, Inc.) according to the manufacturer's instructions. ..

    Tissue:

    Article Title: First evidence of Tahyna virus in Central Italy and seasonal dynamics of its putative vector, Aedes caspius.
    Article Snippet: Mosquito pools were suspended in 0.9 ml of ATL buffer (Animal Tissue Lysis buffer, Qiagen) containing a 5 mm stainless steel bead. .. All samples were homogenised by Tissue Lyser II (QIAGEN, GmbH, Hilden, Germany), followed by a centrifugation at 16,000 g. A 400 μl volume of the supernatant was used for the nucleic acid extraction, using the automatic extractor QIAsymphony (DSP Virus/Pathogen Kit, Qiagen, GmbH, Hilden, Germany) according to the manufacturer’s instructions. .. Pools were first subjected to cDNA synthesis, which was carried out using 20 μl of RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific), following the manufacturer’s instructions, using the Gene AmpR PCR System 9700 (Applied Biosystems, Thermo Fisher Scientific, Waltham) with a thermal cycling profile of 10 min at 25 ◦C and 45 min at 37 ◦C.

    Centrifugation:

    Article Title: First evidence of Tahyna virus in Central Italy and seasonal dynamics of its putative vector, Aedes caspius.
    Article Snippet: Mosquito pools were suspended in 0.9 ml of ATL buffer (Animal Tissue Lysis buffer, Qiagen) containing a 5 mm stainless steel bead. .. All samples were homogenised by Tissue Lyser II (QIAGEN, GmbH, Hilden, Germany), followed by a centrifugation at 16,000 g. A 400 μl volume of the supernatant was used for the nucleic acid extraction, using the automatic extractor QIAsymphony (DSP Virus/Pathogen Kit, Qiagen, GmbH, Hilden, Germany) according to the manufacturer’s instructions. .. Pools were first subjected to cDNA synthesis, which was carried out using 20 μl of RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific), following the manufacturer’s instructions, using the Gene AmpR PCR System 9700 (Applied Biosystems, Thermo Fisher Scientific, Waltham) with a thermal cycling profile of 10 min at 25 ◦C and 45 min at 37 ◦C.

    Nucleic Acid Purification:

    Article Title: First evidence of Tahyna virus in Central Italy and seasonal dynamics of its putative vector, Aedes caspius.
    Article Snippet: Mosquito pools were suspended in 0.9 ml of ATL buffer (Animal Tissue Lysis buffer, Qiagen) containing a 5 mm stainless steel bead. .. All samples were homogenised by Tissue Lyser II (QIAGEN, GmbH, Hilden, Germany), followed by a centrifugation at 16,000 g. A 400 μl volume of the supernatant was used for the nucleic acid extraction, using the automatic extractor QIAsymphony (DSP Virus/Pathogen Kit, Qiagen, GmbH, Hilden, Germany) according to the manufacturer’s instructions. .. Pools were first subjected to cDNA synthesis, which was carried out using 20 μl of RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific), following the manufacturer’s instructions, using the Gene AmpR PCR System 9700 (Applied Biosystems, Thermo Fisher Scientific, Waltham) with a thermal cycling profile of 10 min at 25 ◦C and 45 min at 37 ◦C.

    Automatic:

    Article Title: First evidence of Tahyna virus in Central Italy and seasonal dynamics of its putative vector, Aedes caspius.
    Article Snippet: Mosquito pools were suspended in 0.9 ml of ATL buffer (Animal Tissue Lysis buffer, Qiagen) containing a 5 mm stainless steel bead. .. All samples were homogenised by Tissue Lyser II (QIAGEN, GmbH, Hilden, Germany), followed by a centrifugation at 16,000 g. A 400 μl volume of the supernatant was used for the nucleic acid extraction, using the automatic extractor QIAsymphony (DSP Virus/Pathogen Kit, Qiagen, GmbH, Hilden, Germany) according to the manufacturer’s instructions. .. Pools were first subjected to cDNA synthesis, which was carried out using 20 μl of RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific), following the manufacturer’s instructions, using the Gene AmpR PCR System 9700 (Applied Biosystems, Thermo Fisher Scientific, Waltham) with a thermal cycling profile of 10 min at 25 ◦C and 45 min at 37 ◦C.

    Analysis:

    Article Title: Association of alcohol consumption with oral high-risk human papillomavirus infection: a cross-sectional study within the multinational HPV infection in men (HIM) cohort
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from ORG cell pellets for HPV analysis using the QIASymphony DSP Virus/Pathogen Mini Kit on the QIASymphony SP automated extraction machine (QIAGEN, Inc.) according to the manufacturer's instructions. ..



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    Image Search Results


    (A) Schematic overview of the protocol modifications incorporated into Rapid-RPIP v1 relative to the Standard RPIP workflow. Individual optimisation steps, selected to reduce turnaround time, and their estimated time savings are shown. (B) Relative abundance (%) of the ten ZymoBIOMICS-DNA/RNA mock community organisms recovered by two extraction methods (Quick-DNA/RNA, ZymoBIOMICS-DNA/RNA; mean of 2 replicates each) compared with the manufacturer theoretical composition. (C) RPKM concordance between standard and rapid cDNA synthesis protocols for RNA respiratory viruses (n = 6 organism–sample pairs). Dashed line: identity (y = x); solid line: linear regression with 95% CI. Spearman ρ and Pearson r (log₁₀-RPKM) annotated. (D) Coefficient of variation (log₁₀ scale) of RPKM values across three hybridisation times (30/60/90 min), grouped by pathogen type (Bacteria, RNA Virus, DNA Virus); n per group annotated.

    Journal: medRxiv

    Article Title: Same-day hybrid capture workflow for diagnosis and surveillance of respiratory pathogens

    doi: 10.64898/2026.10.09.26365131

    Figure Lengend Snippet: (A) Schematic overview of the protocol modifications incorporated into Rapid-RPIP v1 relative to the Standard RPIP workflow. Individual optimisation steps, selected to reduce turnaround time, and their estimated time savings are shown. (B) Relative abundance (%) of the ten ZymoBIOMICS-DNA/RNA mock community organisms recovered by two extraction methods (Quick-DNA/RNA, ZymoBIOMICS-DNA/RNA; mean of 2 replicates each) compared with the manufacturer theoretical composition. (C) RPKM concordance between standard and rapid cDNA synthesis protocols for RNA respiratory viruses (n = 6 organism–sample pairs). Dashed line: identity (y = x); solid line: linear regression with 95% CI. Spearman ρ and Pearson r (log₁₀-RPKM) annotated. (D) Coefficient of variation (log₁₀ scale) of RPKM values across three hybridisation times (30/60/90 min), grouped by pathogen type (Bacteria, RNA Virus, DNA Virus); n per group annotated.

    Article Snippet: To this end, the ZymoBIOMICS DNA/RNA Miniprep Kit for nucleic acid extraction was replaced with the Quick-DNA/RNA Pathogen Miniprep Kit (Zymo Research, R1042), and samples were processed according to the manufacturer’s instructions.

    Techniques: Extraction, cDNA Synthesis, Hybridization, Bacteria, Virus