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dsp dna virus pathogen kit  (Qiagen)


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    Structured Review

    Qiagen dsp dna virus pathogen kit
    Dsp Dna Virus Pathogen Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathogens/QIAsymphony+DSP+Virus%2FPathogen+Mini+Kit/med_rxiv__64898__2026__10__07__26364990-47-11-16
    Average 97 stars, based on 642 article reviews
    dsp dna virus pathogen kit - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Extraction:

    Article Title: Development and Validation of Amplicon‐Based Protocol for Sequencing of Respiratory Syncytial Virus Genome
    Article Snippet: .. Viral genome extraction was performed on the QIAsymphony platform using the “Virus Pathogens DSP Midi Kit” (Qiagen, Heidelberg, Germany), starting from a volume of 400 μL with a final elution of 60 μL. ..

    Article Title: COVID-19 Infection in Pediatric Patients Presenting to a Tertiary Center in Jordan: Clinical Characteristics and Age-Related Patterns
    Article Snippet: Nasopharyngeal swabs were collected using the universal transport and preservation of Copan media and stored at −20 ◦C. .. Conventional real-time PCR, specifically the FTD Respiratory Pathogens 21 Assay Kit and EZ 1 and 2 Virus Mini Kit V2.0 by Qiagen (Hilden, Germany), was employed for nucleic acid extraction. ..

    Virus:

    Article Title: Development and Validation of Amplicon‐Based Protocol for Sequencing of Respiratory Syncytial Virus Genome
    Article Snippet: .. Viral genome extraction was performed on the QIAsymphony platform using the “Virus Pathogens DSP Midi Kit” (Qiagen, Heidelberg, Germany), starting from a volume of 400 μL with a final elution of 60 μL. ..

    Article Title: COVID-19 Infection in Pediatric Patients Presenting to a Tertiary Center in Jordan: Clinical Characteristics and Age-Related Patterns
    Article Snippet: Nasopharyngeal swabs were collected using the universal transport and preservation of Copan media and stored at −20 ◦C. .. Conventional real-time PCR, specifically the FTD Respiratory Pathogens 21 Assay Kit and EZ 1 and 2 Virus Mini Kit V2.0 by Qiagen (Hilden, Germany), was employed for nucleic acid extraction. ..

    other:

    Article Title: Development of Low-Cost In-House Assays for Quantitative Detection of HBsAg, HBeAg, and HBV DNA to Enhance Hepatitis B Virus Diagnostics and Antiviral Screening in Resource-Limited Settings.
    Article Snippet: Pathogens 2025, 14, 258 12 of 21 Pathogens 2025, 14, x FOR PEER REVIEW 11 of 21 DNA isolation (i.e., Qiagen DNeasy Blood and Tissue, Hilden, Germany) [14,25].

    Article Title: Development of Low-Cost In-House Assays for Quantitative Detection of HBsAg, HBeAg, and HBV DNA to Enhance Hepatitis B Virus Diagnostics and Antiviral Screening in Resource-Limited Settings.
    Article Snippet: To determine the extraction efficiency, we spiked 50 μL of healthy plasma with known copies of HBV DNA (10–107 copies/μL; 1.8 × 103–1.8 × 109 IU/mL) and extracted the DNA using either the Qiagen silica-column- Pathogens 2025, 14, 258 5 of 21 based kit or column-free DNA isolation, as described above, and eluted the samples in 50 μL of DNase-free water.

    Article Title: First report of <i>Orthotospovirus iridimaculaflavi</i> (Iris yellow spot virus) and <i>Becurtovirus spinaciae</i> (Spinach curly top Arizona virus) in sugarbeet in Idaho, United States
    Article Snippet: First report of Orthotospovirus iridimaculaflavi (Iris yellow spot virus) and Becurtovirus spinaciae (Spinach curly top Arizona virus) in sugarbeet in Idaho, United States

    Article Title: Increasing Number of Clinically Severe Mycoplasma pneumoniae Infections in Children After the COVID-19 Pandemic: A Single-Center Case Series.
    Article Snippet: 1Department of Paediatric Infectious Diseases, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK, 2Department of Microbiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK, 3Department of Neurology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK, 4Developmental Neurosciences Department, University College Longon, London, UK, 5Respiratory Unit, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK, 6Paediatric Intensive Care Unit, Great Ormond Street Hospital for Children, London, UK, 7Department of Cardiac Intensive Care, Great Ormond Street Hospital for Children, London, UK, 8Department of Paediatric Cardiology, Great Ormond Street Hospital for Children, London, UK, 9MRC Centre for Medical Mycology, University of Exeter, Exeter, UK, 10Infection, Immunity, and Inflammation Department, University College London, Great Ormond Street Institute of Child Health, London, UK *Corresponding Author: Seilesh Kadambari, MRCPCH, PhD, Department of Paediatric Infectious Diseases, Great Ormond Street Hospital, Great Ormond Street, London WC1N 3JH, UK.. E-mail: Seilesh.kadambari@gosh.nhs.uk.

    Real-time Polymerase Chain Reaction:

    Article Title: COVID-19 Infection in Pediatric Patients Presenting to a Tertiary Center in Jordan: Clinical Characteristics and Age-Related Patterns
    Article Snippet: Nasopharyngeal swabs were collected using the universal transport and preservation of Copan media and stored at −20 ◦C. .. Conventional real-time PCR, specifically the FTD Respiratory Pathogens 21 Assay Kit and EZ 1 and 2 Virus Mini Kit V2.0 by Qiagen (Hilden, Germany), was employed for nucleic acid extraction. ..

    Biomarker Discovery:

    Article Title: Portable molecular diagnostic platform for rapid point-of-care detection of mpox and other diseases
    Article Snippet: .. Consequently, NAATs, such as quantitative PCR (qPCR), have remained the gold standard for mpox diagnosis due to their excellent specificity and sensitivity.34 Despite these advantages, however, qPCR presents notable limitations, predominantly in POC and near-patient use.35 A selection from the limited number of commercially available near-POC and true-POC molecular mpox diagnostics are listed in Supplementary Table 1, as identified by FIND.36 These examples include PCR panels targeting MPXV, OPXV, or other pathogens (such as VZV, or HSV) that are then paired with expensive automated systems such as Cepheid GeneXpert®, QIAGEN QIAStat-Dx Analyzer, Ustar EasyNAT, or Wondfo U-Card Dx. ..

    Selection:

    Article Title: Portable molecular diagnostic platform for rapid point-of-care detection of mpox and other diseases
    Article Snippet: .. Consequently, NAATs, such as quantitative PCR (qPCR), have remained the gold standard for mpox diagnosis due to their excellent specificity and sensitivity.34 Despite these advantages, however, qPCR presents notable limitations, predominantly in POC and near-patient use.35 A selection from the limited number of commercially available near-POC and true-POC molecular mpox diagnostics are listed in Supplementary Table 1, as identified by FIND.36 These examples include PCR panels targeting MPXV, OPXV, or other pathogens (such as VZV, or HSV) that are then paired with expensive automated systems such as Cepheid GeneXpert®, QIAGEN QIAStat-Dx Analyzer, Ustar EasyNAT, or Wondfo U-Card Dx. ..

    Polymerase Chain Reaction:

    Article Title: Portable molecular diagnostic platform for rapid point-of-care detection of mpox and other diseases
    Article Snippet: .. Consequently, NAATs, such as quantitative PCR (qPCR), have remained the gold standard for mpox diagnosis due to their excellent specificity and sensitivity.34 Despite these advantages, however, qPCR presents notable limitations, predominantly in POC and near-patient use.35 A selection from the limited number of commercially available near-POC and true-POC molecular mpox diagnostics are listed in Supplementary Table 1, as identified by FIND.36 These examples include PCR panels targeting MPXV, OPXV, or other pathogens (such as VZV, or HSV) that are then paired with expensive automated systems such as Cepheid GeneXpert®, QIAGEN QIAStat-Dx Analyzer, Ustar EasyNAT, or Wondfo U-Card Dx. ..



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    (A) Schematic overview of the protocol modifications incorporated into Rapid-RPIP v1 relative to the Standard RPIP workflow. Individual optimisation steps, selected to reduce turnaround time, and their estimated time savings are shown. (B) Relative abundance (%) of the ten ZymoBIOMICS-DNA/RNA mock community organisms recovered by two extraction methods <t>(Quick-DNA/RNA,</t> ZymoBIOMICS-DNA/RNA; mean of 2 replicates each) compared with the manufacturer theoretical composition. (C) RPKM concordance between standard and rapid cDNA synthesis protocols for RNA respiratory viruses (n = 6 organism–sample pairs). Dashed line: identity (y = x); solid line: linear regression with 95% CI. Spearman ρ and Pearson r (log₁₀-RPKM) annotated. (D) Coefficient of variation (log₁₀ scale) of RPKM values across three hybridisation times (30/60/90 min), grouped by pathogen type (Bacteria, RNA Virus, DNA Virus); n per group annotated.
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    Image Search Results


    (A) Schematic overview of the protocol modifications incorporated into Rapid-RPIP v1 relative to the Standard RPIP workflow. Individual optimisation steps, selected to reduce turnaround time, and their estimated time savings are shown. (B) Relative abundance (%) of the ten ZymoBIOMICS-DNA/RNA mock community organisms recovered by two extraction methods (Quick-DNA/RNA, ZymoBIOMICS-DNA/RNA; mean of 2 replicates each) compared with the manufacturer theoretical composition. (C) RPKM concordance between standard and rapid cDNA synthesis protocols for RNA respiratory viruses (n = 6 organism–sample pairs). Dashed line: identity (y = x); solid line: linear regression with 95% CI. Spearman ρ and Pearson r (log₁₀-RPKM) annotated. (D) Coefficient of variation (log₁₀ scale) of RPKM values across three hybridisation times (30/60/90 min), grouped by pathogen type (Bacteria, RNA Virus, DNA Virus); n per group annotated.

    Journal: medRxiv

    Article Title: Same-day hybrid capture workflow for diagnosis and surveillance of respiratory pathogens

    doi: 10.64898/2026.10.09.26365131

    Figure Lengend Snippet: (A) Schematic overview of the protocol modifications incorporated into Rapid-RPIP v1 relative to the Standard RPIP workflow. Individual optimisation steps, selected to reduce turnaround time, and their estimated time savings are shown. (B) Relative abundance (%) of the ten ZymoBIOMICS-DNA/RNA mock community organisms recovered by two extraction methods (Quick-DNA/RNA, ZymoBIOMICS-DNA/RNA; mean of 2 replicates each) compared with the manufacturer theoretical composition. (C) RPKM concordance between standard and rapid cDNA synthesis protocols for RNA respiratory viruses (n = 6 organism–sample pairs). Dashed line: identity (y = x); solid line: linear regression with 95% CI. Spearman ρ and Pearson r (log₁₀-RPKM) annotated. (D) Coefficient of variation (log₁₀ scale) of RPKM values across three hybridisation times (30/60/90 min), grouped by pathogen type (Bacteria, RNA Virus, DNA Virus); n per group annotated.

    Article Snippet: To this end, the ZymoBIOMICS DNA/RNA Miniprep Kit for nucleic acid extraction was replaced with the Quick-DNA/RNA Pathogen Miniprep Kit (Zymo Research, R1042), and samples were processed according to the manufacturer’s instructions.

    Techniques: Extraction, cDNA Synthesis, Hybridization, Bacteria, Virus