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array based oligonucleotide synthesis  (Twist Bioscience)


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    Twist Bioscience array based oligonucleotide synthesis
    Array Based Oligonucleotide Synthesis, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligonucleotide+array/array+based+oligonucleotide+synthesis/pmc13174209-313-7-10
    Average 86 stars, based on 1 article reviews
    array based oligonucleotide synthesis - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Synthesized:

    Article Title: TargetGAN: A generative AI framework for the design of plant core promoters with targeted activity
    Article Snippet: .. These 200-bp candidate sequences were synthesized via array-based oligonucleotide synthesis (Twist Bioscience). ..

    Article Title: Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes
    Article Snippet: .. The designed 5′UTR-BC library was synthesized via oligo array synthesis by Twist Bioscience. ssDNA oligos were amplified with 12 cycles using NEBNext High-Fidelity 2× PCR Master Mix using one of four primer sets to enrich a specific library subset (primers in ). .. Following PCR column cleanup, the PCR products were digested with BamHI and SalI, size selected with a 1.8:1 SPRI bead:sample cleanup (Omega Bio-tek), and ligated into pJD486 (Addgene number: pending) which comprised the following features: flanking ITRs for AAV packaging, a CMV promoter 32 bp upstream of the BamHI restriction site, and an hGH terminator.

    Article Title: Approaches for identification of 5' UTR mutations impacting translation and protein production from neurodevelopmental disorder genes.
    Article Snippet: .. The designed 5 ′ UTR-BC library was synthesized via oligo array synthesis by Twist Bioscience. ssDNA oligos were amplified with 12 cycles using NEBNext High-Fidelity 2× PCR Master Mix using one of four primer sets to enrich a specific library subset (primers in Table S6). .. Following PCR column cleanup, the PCR products were digested with BamHI and SalI, size selected with a 1.8:1 SPRI bead:sample cleanup (Omega Bio-tek), and ligated into pJD486 (Addgene number: pending) which comprised the following features: flanking ITRs for AAV packaging, a CMV promoter 32 bp upstream of the BamHI restriction site, and an hGH terminator.

    Oligonucleotide Synthesis:

    Article Title: TargetGAN: A generative AI framework for the design of plant core promoters with targeted activity
    Article Snippet: .. These 200-bp candidate sequences were synthesized via array-based oligonucleotide synthesis (Twist Bioscience). ..

    Sequencing:

    Article Title: A compendium of synthetic lethal gene pairs defined by extensive combinatorial pan-cancer CRISPR screening
    Article Snippet: .. Oligo sequences were ordered from TWIST Bioscience with the following sequence and designed to modify the second scaffold to a sequence taken from Shen et al., [ ]. .. We resuspended the synthesised TWIST oligo pool to a concentration of 0.01 ng/μl and amplified it using Q5 High-Fidelity 2X Master Mix (NEB, #M0492).

    Biomarker Discovery:

    Article Title: DNA-MGC+: A versatile codec for reliable and resource-efficient data storage on synthetic DNA
    Article Snippet: Notable results include supporting reliable decoding under base-level error rates of up to 24% in synthetic scenarios, achieving an estimated storage density of 57 EB/g under experimentally derived error and bias profiles, and enabling reliable retrieval under both Illumina and Nanopore sequencing at sequencing depths below 3×, with associated read costs below 3.5 bits/nt. .. While most previous studies evaluate DNA storage codecs under high-fidelity workflows, typically involving material deposition-based synthesis (e.g., Twist Bioscience) and/or Illumina sequencing, our experimental validation also includes results combining electrochemical synthesis (GenScript) with Nanopore sequencing, both of which are substantially more error-prone than their conventional counterparts. ..

    Nanopore Sequencing:

    Article Title: DNA-MGC+: A versatile codec for reliable and resource-efficient data storage on synthetic DNA
    Article Snippet: Notable results include supporting reliable decoding under base-level error rates of up to 24% in synthetic scenarios, achieving an estimated storage density of 57 EB/g under experimentally derived error and bias profiles, and enabling reliable retrieval under both Illumina and Nanopore sequencing at sequencing depths below 3×, with associated read costs below 3.5 bits/nt. .. While most previous studies evaluate DNA storage codecs under high-fidelity workflows, typically involving material deposition-based synthesis (e.g., Twist Bioscience) and/or Illumina sequencing, our experimental validation also includes results combining electrochemical synthesis (GenScript) with Nanopore sequencing, both of which are substantially more error-prone than their conventional counterparts. ..

    Amplification:

    Article Title: Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes
    Article Snippet: .. The designed 5′UTR-BC library was synthesized via oligo array synthesis by Twist Bioscience. ssDNA oligos were amplified with 12 cycles using NEBNext High-Fidelity 2× PCR Master Mix using one of four primer sets to enrich a specific library subset (primers in ). .. Following PCR column cleanup, the PCR products were digested with BamHI and SalI, size selected with a 1.8:1 SPRI bead:sample cleanup (Omega Bio-tek), and ligated into pJD486 (Addgene number: pending) which comprised the following features: flanking ITRs for AAV packaging, a CMV promoter 32 bp upstream of the BamHI restriction site, and an hGH terminator.

    Article Title: Approaches for identification of 5' UTR mutations impacting translation and protein production from neurodevelopmental disorder genes.
    Article Snippet: .. The designed 5 ′ UTR-BC library was synthesized via oligo array synthesis by Twist Bioscience. ssDNA oligos were amplified with 12 cycles using NEBNext High-Fidelity 2× PCR Master Mix using one of four primer sets to enrich a specific library subset (primers in Table S6). .. Following PCR column cleanup, the PCR products were digested with BamHI and SalI, size selected with a 1.8:1 SPRI bead:sample cleanup (Omega Bio-tek), and ligated into pJD486 (Addgene number: pending) which comprised the following features: flanking ITRs for AAV packaging, a CMV promoter 32 bp upstream of the BamHI restriction site, and an hGH terminator.

    Polymerase Chain Reaction:

    Article Title: Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes
    Article Snippet: .. The designed 5′UTR-BC library was synthesized via oligo array synthesis by Twist Bioscience. ssDNA oligos were amplified with 12 cycles using NEBNext High-Fidelity 2× PCR Master Mix using one of four primer sets to enrich a specific library subset (primers in ). .. Following PCR column cleanup, the PCR products were digested with BamHI and SalI, size selected with a 1.8:1 SPRI bead:sample cleanup (Omega Bio-tek), and ligated into pJD486 (Addgene number: pending) which comprised the following features: flanking ITRs for AAV packaging, a CMV promoter 32 bp upstream of the BamHI restriction site, and an hGH terminator.

    Article Title: Approaches for identification of 5' UTR mutations impacting translation and protein production from neurodevelopmental disorder genes.
    Article Snippet: .. The designed 5 ′ UTR-BC library was synthesized via oligo array synthesis by Twist Bioscience. ssDNA oligos were amplified with 12 cycles using NEBNext High-Fidelity 2× PCR Master Mix using one of four primer sets to enrich a specific library subset (primers in Table S6). .. Following PCR column cleanup, the PCR products were digested with BamHI and SalI, size selected with a 1.8:1 SPRI bead:sample cleanup (Omega Bio-tek), and ligated into pJD486 (Addgene number: pending) which comprised the following features: flanking ITRs for AAV packaging, a CMV promoter 32 bp upstream of the BamHI restriction site, and an hGH terminator.

    other:

    Article Title: uSort–M: Scalable isolation of user-defined sequences from diverse pooled libraries
    Article Snippet: Pooled oligonucleotide synthesis from commercial providers (e.g., Twist Biosciences or IDT) is commonly limited to sequences shorter than 350 bp, although recent advances in pooled assembly are extending accessible fragment lengths by engineering orthogonal, pairwise interactions in diverse pools.

    Article Title: De novo design of epitope-specific antibodies via a structure-driven computational workflow.
    Article Snippet: We also extend our gratitude to Twist Biosciences for their oligo synthesis, which was critical for high-throughput experiments.

    Distillation:

    Article Title: Atomically accurate de novo design of antibodies with RFdiffusion.
    Article Snippet: Nathaniel R. Bennett, Joseph L. Watson1,2,21 ✉, Robert J. Ragotte1,2,21 ✉, Andrew J. Borst, DéJenaé L. See, Connor Weidle, Riti Biswas, Yutong Yu, Ellen L. Shrock, Russell Ault, Philip J. Y. Leung, Buwei Huang, Inna Goreshnik, John Tam, Kenneth D. Carr, Benedikt Singer, Cameron Criswell, Basile I. M. Wicky, Dionne Vafeados, Mariana Garcia Sanchez, Ho Min Kim, Susana Vázquez Torres, Sidney Chan, Shirley M. Sun, Timothy T. Spear, Yi Sun, Keelan O’Reilly, John M. Maris, Nikolaos G. Sgourakis, Roman A. Melnyk, Chang C. Liu & David Baker1,2,10 ✉

    Recombinant:

    Article Title: Atomically accurate de novo design of antibodies with RFdiffusion.
    Article Snippet: Nathaniel R. Bennett, Joseph L. Watson1,2,21 ✉, Robert J. Ragotte1,2,21 ✉, Andrew J. Borst, DéJenaé L. See, Connor Weidle, Riti Biswas, Yutong Yu, Ellen L. Shrock, Russell Ault, Philip J. Y. Leung, Buwei Huang, Inna Goreshnik, John Tam, Kenneth D. Carr, Benedikt Singer, Cameron Criswell, Basile I. M. Wicky, Dionne Vafeados, Mariana Garcia Sanchez, Ho Min Kim, Susana Vázquez Torres, Sidney Chan, Shirley M. Sun, Timothy T. Spear, Yi Sun, Keelan O’Reilly, John M. Maris, Nikolaos G. Sgourakis, Roman A. Melnyk, Chang C. Liu & David Baker1,2,10 ✉

    Next-Generation Sequencing:

    Article Title: Atomically accurate de novo design of antibodies with RFdiffusion.
    Article Snippet: Nathaniel R. Bennett, Joseph L. Watson1,2,21 ✉, Robert J. Ragotte1,2,21 ✉, Andrew J. Borst, DéJenaé L. See, Connor Weidle, Riti Biswas, Yutong Yu, Ellen L. Shrock, Russell Ault, Philip J. Y. Leung, Buwei Huang, Inna Goreshnik, John Tam, Kenneth D. Carr, Benedikt Singer, Cameron Criswell, Basile I. M. Wicky, Dionne Vafeados, Mariana Garcia Sanchez, Ho Min Kim, Susana Vázquez Torres, Sidney Chan, Shirley M. Sun, Timothy T. Spear, Yi Sun, Keelan O’Reilly, John M. Maris, Nikolaos G. Sgourakis, Roman A. Melnyk, Chang C. Liu & David Baker1,2,10 ✉

    Oligo Synthesis:

    Article Title: Atomically accurate de novo design of antibodies with RFdiffusion.
    Article Snippet: Nathaniel R. Bennett, Joseph L. Watson1,2,21 ✉, Robert J. Ragotte1,2,21 ✉, Andrew J. Borst, DéJenaé L. See, Connor Weidle, Riti Biswas, Yutong Yu, Ellen L. Shrock, Russell Ault, Philip J. Y. Leung, Buwei Huang, Inna Goreshnik, John Tam, Kenneth D. Carr, Benedikt Singer, Cameron Criswell, Basile I. M. Wicky, Dionne Vafeados, Mariana Garcia Sanchez, Ho Min Kim, Susana Vázquez Torres, Sidney Chan, Shirley M. Sun, Timothy T. Spear, Yi Sun, Keelan O’Reilly, John M. Maris, Nikolaos G. Sgourakis, Roman A. Melnyk, Chang C. Liu & David Baker1,2,10 ✉



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