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Cellecta Inc arrayed oligo synthesis pools
Arrayed Oligo Synthesis Pools, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo+array/arrayed+oligo+synthesis+pools/pm40588516-322-39-42
Average 90 stars, based on 1 article reviews
arrayed oligo synthesis pools - by Bioz Stars, 2026-10
90/100 stars

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CRISPR:

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
Article Snippet: Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. In brief, GNE293 line was plated in plates coated with 0.2% gelatine (Science Cell, Catalog No. 0423) and cultured in DMEM high glucose media (Thermo Fisher, Catalog No. 35050061) supplementedwithMEMNon-Essential AminoAcids Solution (Thermo Fisher, Catalog No. 11140050) and Glutamax (Thermo Fisher, Catalog No. 35050061).

Plasmid Preparation:

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
Article Snippet: Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. In brief, GNE293 line was plated in plates coated with 0.2% gelatine (Science Cell, Catalog No. 0423) and cultured in DMEM high glucose media (Thermo Fisher, Catalog No. 35050061) supplementedwithMEMNon-Essential AminoAcids Solution (Thermo Fisher, Catalog No. 11140050) and Glutamax (Thermo Fisher, Catalog No. 35050061).

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
Article Snippet: .. Briefly, 4 sub-libraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. ..

Article Title: CRISPR whole-genome screening identifies new necroptosis regulators and RIPK1 alternative splicing.
Article Snippet: .. The sequences were produced in nine arrayed oligo synthesis pools (Cellecta, Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma) termed “library vector” in the subsequent text. ..

Produced:

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
Article Snippet: Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. In brief, GNE293 line was plated in plates coated with 0.2% gelatine (Science Cell, Catalog No. 0423) and cultured in DMEM high glucose media (Thermo Fisher, Catalog No. 35050061) supplementedwithMEMNon-Essential AminoAcids Solution (Thermo Fisher, Catalog No. 11140050) and Glutamax (Thermo Fisher, Catalog No. 35050061).

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
Article Snippet: .. Briefly, 4 sub-libraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. ..

Article Title: CRISPR whole-genome screening identifies new necroptosis regulators and RIPK1 alternative splicing.
Article Snippet: .. The sequences were produced in nine arrayed oligo synthesis pools (Cellecta, Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma) termed “library vector” in the subsequent text. ..

Oligo Synthesis:

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.
Article Snippet: Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. Pooled CRISPR sgRNA vector library production The lentiviral library has been produced as in ref. 63 Briefly, 4 sublibraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. .. In brief, GNE293 line was plated in plates coated with 0.2% gelatine (Science Cell, Catalog No. 0423) and cultured in DMEM high glucose media (Thermo Fisher, Catalog No. 35050061) supplementedwithMEMNon-Essential AminoAcids Solution (Thermo Fisher, Catalog No. 11140050) and Glutamax (Thermo Fisher, Catalog No. 35050061).

Article Title: A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity
Article Snippet: .. Briefly, 4 sub-libraries were produced (library1 sgRNAs: 18,991; library 2 sgRNAs: 18,990; library 3 sgRNA: 18,986; library 4 sgRNA: 18,975) as four arrayed oligo synthesis pools (Cellecta Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma-Aldrich) that contained an optimized sgRNA scaffold to generate pool library vectors. ..

Article Title: CRISPR whole-genome screening identifies new necroptosis regulators and RIPK1 alternative splicing.
Article Snippet: .. The sequences were produced in nine arrayed oligo synthesis pools (Cellecta, Inc.) and sub-cloned into a puromycin selectable lentivirus vector pLKO_SHC201 (Sigma) termed “library vector” in the subsequent text. ..



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