nycodenz separation solution (Beijing Solarbio Science)
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Structured Review
Beijing Solarbio Science
nycodenz separation solution
Nycodenz Separation Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nycodenz+solution/nycodenz+separation+solution/pmc06726602-40-15-21
Average 90 stars, based on 1 article reviews
Nycodenz Separation Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nycodenz+solution/nycodenz+separation+solution/pmc06726602-40-15-21
Average 90 stars, based on 1 article reviews
nycodenz separation solution - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "A novel technique to prepare a single cell suspension of isolated quiescent human hepatic stellate cells"
Article Title: A novel technique to prepare a single cell suspension of isolated quiescent human hepatic stellate cells
Journal: Scientific Reports
doi: 10.1038/s41598-019-49287-7
Figure Legend Snippet: Schematic overview of the protocol for isolation of human HSC. ( a ) Surgically removed fresh normal liver tissues were processed and collected in an ice box at 4 °C under aseptic conditions. ( b ) The connective tissues, bile ducts, and blood vessels were trimmed away as much as possible using ophthalmic scissors, and the tissue were cut into small approximately 1-mm pieces. ( c ) Gentle MACSTM tissue processor disrupt the tissue in a gentle and stable setting. ( d ) Stop digestion and filtered. ( e ) Isolation human hepatic cells and human hepatic stellate cells. ( f ) Nycodenz density gradient centrifugation.
Techniques Used: Isolation, Gentle, Gradient Centrifugation
Figure Legend Snippet: ( a ) The solution was filtered through a SmartStrainer filter. ( b ) Isolate the hepatocytes. ( c ) The pellet containing the HSCs. ( d ) DMEM washing pellet. ( e ) Preparation of 15 ml tubes for Nycodenz density centrifugation. ( f ) After density centrifugation, HSCs can be identified as a white cell layer that is floating at the surface of the gradient. ( g ) The HSC-containing layers are collected and combined, filled with GASS, and centrifuged again. After centrifugation, a white pellet is visible.
Techniques Used: Centrifugation
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