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iohexol solutions  (Thermo Fisher)


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    Thermo Fisher iohexol solutions
    Iohexol Solutions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nycodenz+solution/IOHEXOL/pm41619262-156-3-19
    Average 94 stars, based on 1 article reviews
    iohexol solutions - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Dysregulation of SRSF3/ circSAMD4 /CIRBP Axis Promotes Iodinated Contrast-induced Acute Kidney Injury
    Article Snippet: The immortalized proximal RTEC line (HK-2 cells) initially from ATCC and 293T cells were maintained in DMEM/F-12 and DMEM respectively, both containing 10% FBS and 1% antibiotics (Gibco, Carlsbad, CA). .. Based on our previous study , HK-2 cells were exposed to 200 mg iodine/mL iohexol for 6 h. For loss-of-function studies, HK-2 cells were transfected with circSAMD4 small interfering RNA (siRNA) (Hanbio Biotechnology, Shanghai, China), SRSF3 siRNA, or CIRBP siRNA (Tsingke Biotechnology, Beijing, China), or negative control using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) as instructed by the manufacturer. ..

    Article Title: A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD11a (LFA-1) BioLegend Cat#301202; clone HI111; RRID: AB_314140 Mouse anti-human CD16 BioLegend Cat#302002; clone 3G8; RRID: AB_314202 Mouse anti-human Granzyme B – Alexa Fluor 647 BD Biosciences Cat#560212; clone GB11; RRID: AB_11154033 Mouse anti-human Vimentin – Alexa Fluor 488 Abcam Cat#ab195877 Goat anti-rabbit – Alexa Fluor 488 Abcam Cat#ab150089 Rabbit anti-human PKC-Q Santa Cruz Biotechnology Cat#sc-212; polyclonal C-18 Bacterial and virus strains LeGO-G2-puro https://www.lentigo-vectors. de/vectors.htm LeGO-G2/Puro pMDLg/pRRE Dull et al.54 Addgene #12251 pRSV-Rev Dull et al.54 Addgene #12253 pCMV-VSV-G Stewart et al.55 Addgene #8454 pLenti-CMV-MCS-RFP-SV-puro Jonathan Garlick and Behzad Gerami-Naini Addgene #109377 pLJM1-EGFP Sancak et al.56 Addgene #19319 pJML1-NES-LQDT-mGFP-P2A-NESVGPD-mCherry Beaudouin et al.57 N/A Biological samples Fresh buffy coats from healthy donors Karolinska University Hospital N/A Chemicals, peptides, and recombinant proteins Calcium Phosphate Transfection Kit Sigma-Aldrich CAPHOS Chloroquine Sigma-Aldrich Cat#C6628 Protamine sulfate Sigma-Aldrich Cat#P3369 Puromycin ThermoFisher Scientific Cat#A1113803 Interleukin-2 PeproTech Cat#200-02 Interleukin-15 R&D Systems Cat#247-ILB-005 D-Glucose Sigma-Aldrich Cat#D8375 D-Mannitol Sigma-Aldrich Cat#M4125 L-glutamine Sigma-Aldrich Cat#G7513 Lactic acid Sigma-Aldrich Cat#L6402 Anti-adherence rinsing solution STEMCELL Technologies Cat#07010 BD Pharmingen DRAQ5 BD Biosciences Cat#564902 Abberior LIVE 590 Tubulin Abberior LV590-0141 Phalloidin-STAR Red Abberior STRED-0100 CellTrace Yellow ThermoFisher Scientific Cat#C34567 Cytofix/Cytoperm BD Biosciences Cat#554714 Cytofix BD Biosciences Cat#554655 Perm/Wash BD Bioscences Cat#554714 Histodenz/Omnipaque (iohexol) Apoteket Cat#582692 Critical commercial assays TetraSpeck fluorescent microspheres kit Invitrogen Cat#T7284 Lenti-X Concentrator Takara Cat#631231 EasySep Human NK Cell Isolation Kit STEMCELL Technologies Cat#17955 (Continued on next page) e1 Cell Reports Methods 5, 100965, January 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Human: A498 cell line ATCC HTB-44 Human: OVCAR-8 cell line Gift from Brinton Seashore-Ludlow (Karolinska Institutet, Stockholm, Sweden) N/A Human: DLD-1 cell line ATCC CCL-221 Human: ACHN cell line ATCC CRL-1611 Human: K562 cell line ATCC CCL-243 Human: Jurkat cell line ATCC TIB-152 Human: Nalm-6 cell line DSMZ ACC 128 Human: SUP-B15 cell line Gift from Rozbeh Jafari (Karolinska Institutet, Stockholm, Sweden) N/A Human: Kasumi-1 cell line ATCC CRL-2724 Human: MCF-7 cell line ATCC HTB-22 Human: HeLa cell line ATCC CCL-2 Human: IMR-32 cell line Gift from Jakob Stenman (Karolinska Institutet, Stockholm) N/A Human: A549.Histone.mKate2 cell line Gift from Kalle Malmberg (Oslo University Hospital, Oslo, Norway) N/A Software and algorithms Prism GraphPad https://graphpad.com ImageJ Schneider et al. https://fiji.sc ilastik Berg et al.58 https://www.ilastik.org Imaris Oxford Instruments https://imaris.oxinst.com Moldex3D CoreTech System https://www.moldex3d.com MATLAB 2023B MathWorks https://se.mathworks.com/products/matlab.html measurePSF Rob Campbell https://github.com/SWC-Advanced- Microscopy/measurePSF BioRender BioRender https://biorender.com Image segmentation networks Deep learning Toolbox for resnet50 from MathWorks https://se.mathworks.com/help/ deeplearning/ref/resnet50.html Cell detection scripts for microwell image data This paper https://zenodo.org/records/14507473 https://doi.org/10.5281/zenodo.14507473 OPEN ACCESS

    Small Interfering RNA:

    Article Title: Dysregulation of SRSF3/ circSAMD4 /CIRBP Axis Promotes Iodinated Contrast-induced Acute Kidney Injury
    Article Snippet: The immortalized proximal RTEC line (HK-2 cells) initially from ATCC and 293T cells were maintained in DMEM/F-12 and DMEM respectively, both containing 10% FBS and 1% antibiotics (Gibco, Carlsbad, CA). .. Based on our previous study , HK-2 cells were exposed to 200 mg iodine/mL iohexol for 6 h. For loss-of-function studies, HK-2 cells were transfected with circSAMD4 small interfering RNA (siRNA) (Hanbio Biotechnology, Shanghai, China), SRSF3 siRNA, or CIRBP siRNA (Tsingke Biotechnology, Beijing, China), or negative control using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) as instructed by the manufacturer. ..

    Negative Control:

    Article Title: Dysregulation of SRSF3/ circSAMD4 /CIRBP Axis Promotes Iodinated Contrast-induced Acute Kidney Injury
    Article Snippet: The immortalized proximal RTEC line (HK-2 cells) initially from ATCC and 293T cells were maintained in DMEM/F-12 and DMEM respectively, both containing 10% FBS and 1% antibiotics (Gibco, Carlsbad, CA). .. Based on our previous study , HK-2 cells were exposed to 200 mg iodine/mL iohexol for 6 h. For loss-of-function studies, HK-2 cells were transfected with circSAMD4 small interfering RNA (siRNA) (Hanbio Biotechnology, Shanghai, China), SRSF3 siRNA, or CIRBP siRNA (Tsingke Biotechnology, Beijing, China), or negative control using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) as instructed by the manufacturer. ..

    Incubation:

    Article Title: Secreted frizzled-related protein 2 monoclonal antibody-mediated IFN-ϒ reprograms tumor-associated macrophages to suppress triple negative breast cancer
    Article Snippet: The tumor pieces were placed into Iscove’s Modified Dulbecco’s Medium (IMDM, Corning, cat # 10-016-CV) and digested with the Tumor Dissociation Kit following manufacturer protocol for soft mouse tumors (Miltenyi Biotec, Auburn CA, cat # 130-096-730). .. After dissociation, the cells were counted and resuspended with the corresponding F4/80 monoclonal antibody volume according to manufacture protocol (ThermoFisher, cat # 13-4801-8, RRID:AB_466658) and incubated for 20 min at room temperature. .. The cells were rinsed in PBS, resuspended in the corresponding volume of Streptavidin beads (BD Biosciences, Franklin Lakes NJ, cat # 557,812), and incubated at 4 °C for 30 min.

    Injection:

    Article Title: Morphology of the glymphatic and meningeal lymphatic structures of the bottlenose dolphin
    Article Snippet: .. The intracranial veins were injected with a mixture of anatomic latex and contrast agents (barium sulphate or Iohexol; see for detailed methods), imaged, dissected at University of Florida College of Veterinary Medicine; Fig. C), and post-processed using Amira for FEI Systems 6.3.0 3D Data Visualization and Analysis Software for Life Sciences ( https://download.amira-avizo-software.com/private/MASTERS/Amira/6.3.0/ad6fe30b/Amira-630-Windows64-VC12.exe ) to examine the 3-D structure of the intracranial veins, including their associated connection and relation to skeletal landmarks. ..

    Software:

    Article Title: Morphology of the glymphatic and meningeal lymphatic structures of the bottlenose dolphin
    Article Snippet: .. The intracranial veins were injected with a mixture of anatomic latex and contrast agents (barium sulphate or Iohexol; see for detailed methods), imaged, dissected at University of Florida College of Veterinary Medicine; Fig. C), and post-processed using Amira for FEI Systems 6.3.0 3D Data Visualization and Analysis Software for Life Sciences ( https://download.amira-avizo-software.com/private/MASTERS/Amira/6.3.0/ad6fe30b/Amira-630-Windows64-VC12.exe ) to examine the 3-D structure of the intracranial veins, including their associated connection and relation to skeletal landmarks. ..

    Single Particle:

    Article Title: CgsiGP1 sample in nanodisc
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry Not Provided Number of particles used 46665 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 60.0 Depositor Minimum defocus (nm) 1.2 Depositor Maximum defocus (nm) 2.5 Depositor Magnification Not provided Image detector FEI FALCON IV (4k x 4k) Depositor Maximum map value 3.980 Depositor Minimum map value -2.065 Depositor Average map value 0.001 Depositor Map value standard deviation 0.109 Depositor Recommended contour level 0.581 Depositor Map size (Å) 409.984, 409.984, 409.984 wwPDB Map dimensions 320, 320, 320 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.2812, 1.2812, 1.2812 Depositor ..

    Microscopy:

    Article Title: CgsiGP1 sample in nanodisc
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry Not Provided Number of particles used 46665 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 60.0 Depositor Minimum defocus (nm) 1.2 Depositor Maximum defocus (nm) 2.5 Depositor Magnification Not provided Image detector FEI FALCON IV (4k x 4k) Depositor Maximum map value 3.980 Depositor Minimum map value -2.065 Depositor Average map value 0.001 Depositor Map value standard deviation 0.109 Depositor Recommended contour level 0.581 Depositor Map size (Å) 409.984, 409.984, 409.984 wwPDB Map dimensions 320, 320, 320 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.2812, 1.2812, 1.2812 Depositor ..

    Standard Deviation:

    Article Title: CgsiGP1 sample in nanodisc
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry Not Provided Number of particles used 46665 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 60.0 Depositor Minimum defocus (nm) 1.2 Depositor Maximum defocus (nm) 2.5 Depositor Magnification Not provided Image detector FEI FALCON IV (4k x 4k) Depositor Maximum map value 3.980 Depositor Minimum map value -2.065 Depositor Average map value 0.001 Depositor Map value standard deviation 0.109 Depositor Recommended contour level 0.581 Depositor Map size (Å) 409.984, 409.984, 409.984 wwPDB Map dimensions 320, 320, 320 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.2812, 1.2812, 1.2812 Depositor ..

    Virus:

    Article Title: A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD11a (LFA-1) BioLegend Cat#301202; clone HI111; RRID: AB_314140 Mouse anti-human CD16 BioLegend Cat#302002; clone 3G8; RRID: AB_314202 Mouse anti-human Granzyme B – Alexa Fluor 647 BD Biosciences Cat#560212; clone GB11; RRID: AB_11154033 Mouse anti-human Vimentin – Alexa Fluor 488 Abcam Cat#ab195877 Goat anti-rabbit – Alexa Fluor 488 Abcam Cat#ab150089 Rabbit anti-human PKC-Q Santa Cruz Biotechnology Cat#sc-212; polyclonal C-18 Bacterial and virus strains LeGO-G2-puro https://www.lentigo-vectors. de/vectors.htm LeGO-G2/Puro pMDLg/pRRE Dull et al.54 Addgene #12251 pRSV-Rev Dull et al.54 Addgene #12253 pCMV-VSV-G Stewart et al.55 Addgene #8454 pLenti-CMV-MCS-RFP-SV-puro Jonathan Garlick and Behzad Gerami-Naini Addgene #109377 pLJM1-EGFP Sancak et al.56 Addgene #19319 pJML1-NES-LQDT-mGFP-P2A-NESVGPD-mCherry Beaudouin et al.57 N/A Biological samples Fresh buffy coats from healthy donors Karolinska University Hospital N/A Chemicals, peptides, and recombinant proteins Calcium Phosphate Transfection Kit Sigma-Aldrich CAPHOS Chloroquine Sigma-Aldrich Cat#C6628 Protamine sulfate Sigma-Aldrich Cat#P3369 Puromycin ThermoFisher Scientific Cat#A1113803 Interleukin-2 PeproTech Cat#200-02 Interleukin-15 R&D Systems Cat#247-ILB-005 D-Glucose Sigma-Aldrich Cat#D8375 D-Mannitol Sigma-Aldrich Cat#M4125 L-glutamine Sigma-Aldrich Cat#G7513 Lactic acid Sigma-Aldrich Cat#L6402 Anti-adherence rinsing solution STEMCELL Technologies Cat#07010 BD Pharmingen DRAQ5 BD Biosciences Cat#564902 Abberior LIVE 590 Tubulin Abberior LV590-0141 Phalloidin-STAR Red Abberior STRED-0100 CellTrace Yellow ThermoFisher Scientific Cat#C34567 Cytofix/Cytoperm BD Biosciences Cat#554714 Cytofix BD Biosciences Cat#554655 Perm/Wash BD Bioscences Cat#554714 Histodenz/Omnipaque (iohexol) Apoteket Cat#582692 Critical commercial assays TetraSpeck fluorescent microspheres kit Invitrogen Cat#T7284 Lenti-X Concentrator Takara Cat#631231 EasySep Human NK Cell Isolation Kit STEMCELL Technologies Cat#17955 (Continued on next page) e1 Cell Reports Methods 5, 100965, January 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Human: A498 cell line ATCC HTB-44 Human: OVCAR-8 cell line Gift from Brinton Seashore-Ludlow (Karolinska Institutet, Stockholm, Sweden) N/A Human: DLD-1 cell line ATCC CCL-221 Human: ACHN cell line ATCC CRL-1611 Human: K562 cell line ATCC CCL-243 Human: Jurkat cell line ATCC TIB-152 Human: Nalm-6 cell line DSMZ ACC 128 Human: SUP-B15 cell line Gift from Rozbeh Jafari (Karolinska Institutet, Stockholm, Sweden) N/A Human: Kasumi-1 cell line ATCC CRL-2724 Human: MCF-7 cell line ATCC HTB-22 Human: HeLa cell line ATCC CCL-2 Human: IMR-32 cell line Gift from Jakob Stenman (Karolinska Institutet, Stockholm) N/A Human: A549.Histone.mKate2 cell line Gift from Kalle Malmberg (Oslo University Hospital, Oslo, Norway) N/A Software and algorithms Prism GraphPad https://graphpad.com ImageJ Schneider et al. https://fiji.sc ilastik Berg et al.58 https://www.ilastik.org Imaris Oxford Instruments https://imaris.oxinst.com Moldex3D CoreTech System https://www.moldex3d.com MATLAB 2023B MathWorks https://se.mathworks.com/products/matlab.html measurePSF Rob Campbell https://github.com/SWC-Advanced- Microscopy/measurePSF BioRender BioRender https://biorender.com Image segmentation networks Deep learning Toolbox for resnet50 from MathWorks https://se.mathworks.com/help/ deeplearning/ref/resnet50.html Cell detection scripts for microwell image data This paper https://zenodo.org/records/14507473 https://doi.org/10.5281/zenodo.14507473 OPEN ACCESS

    Recombinant:

    Article Title: A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD11a (LFA-1) BioLegend Cat#301202; clone HI111; RRID: AB_314140 Mouse anti-human CD16 BioLegend Cat#302002; clone 3G8; RRID: AB_314202 Mouse anti-human Granzyme B – Alexa Fluor 647 BD Biosciences Cat#560212; clone GB11; RRID: AB_11154033 Mouse anti-human Vimentin – Alexa Fluor 488 Abcam Cat#ab195877 Goat anti-rabbit – Alexa Fluor 488 Abcam Cat#ab150089 Rabbit anti-human PKC-Q Santa Cruz Biotechnology Cat#sc-212; polyclonal C-18 Bacterial and virus strains LeGO-G2-puro https://www.lentigo-vectors. de/vectors.htm LeGO-G2/Puro pMDLg/pRRE Dull et al.54 Addgene #12251 pRSV-Rev Dull et al.54 Addgene #12253 pCMV-VSV-G Stewart et al.55 Addgene #8454 pLenti-CMV-MCS-RFP-SV-puro Jonathan Garlick and Behzad Gerami-Naini Addgene #109377 pLJM1-EGFP Sancak et al.56 Addgene #19319 pJML1-NES-LQDT-mGFP-P2A-NESVGPD-mCherry Beaudouin et al.57 N/A Biological samples Fresh buffy coats from healthy donors Karolinska University Hospital N/A Chemicals, peptides, and recombinant proteins Calcium Phosphate Transfection Kit Sigma-Aldrich CAPHOS Chloroquine Sigma-Aldrich Cat#C6628 Protamine sulfate Sigma-Aldrich Cat#P3369 Puromycin ThermoFisher Scientific Cat#A1113803 Interleukin-2 PeproTech Cat#200-02 Interleukin-15 R&D Systems Cat#247-ILB-005 D-Glucose Sigma-Aldrich Cat#D8375 D-Mannitol Sigma-Aldrich Cat#M4125 L-glutamine Sigma-Aldrich Cat#G7513 Lactic acid Sigma-Aldrich Cat#L6402 Anti-adherence rinsing solution STEMCELL Technologies Cat#07010 BD Pharmingen DRAQ5 BD Biosciences Cat#564902 Abberior LIVE 590 Tubulin Abberior LV590-0141 Phalloidin-STAR Red Abberior STRED-0100 CellTrace Yellow ThermoFisher Scientific Cat#C34567 Cytofix/Cytoperm BD Biosciences Cat#554714 Cytofix BD Biosciences Cat#554655 Perm/Wash BD Bioscences Cat#554714 Histodenz/Omnipaque (iohexol) Apoteket Cat#582692 Critical commercial assays TetraSpeck fluorescent microspheres kit Invitrogen Cat#T7284 Lenti-X Concentrator Takara Cat#631231 EasySep Human NK Cell Isolation Kit STEMCELL Technologies Cat#17955 (Continued on next page) e1 Cell Reports Methods 5, 100965, January 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Human: A498 cell line ATCC HTB-44 Human: OVCAR-8 cell line Gift from Brinton Seashore-Ludlow (Karolinska Institutet, Stockholm, Sweden) N/A Human: DLD-1 cell line ATCC CCL-221 Human: ACHN cell line ATCC CRL-1611 Human: K562 cell line ATCC CCL-243 Human: Jurkat cell line ATCC TIB-152 Human: Nalm-6 cell line DSMZ ACC 128 Human: SUP-B15 cell line Gift from Rozbeh Jafari (Karolinska Institutet, Stockholm, Sweden) N/A Human: Kasumi-1 cell line ATCC CRL-2724 Human: MCF-7 cell line ATCC HTB-22 Human: HeLa cell line ATCC CCL-2 Human: IMR-32 cell line Gift from Jakob Stenman (Karolinska Institutet, Stockholm) N/A Human: A549.Histone.mKate2 cell line Gift from Kalle Malmberg (Oslo University Hospital, Oslo, Norway) N/A Software and algorithms Prism GraphPad https://graphpad.com ImageJ Schneider et al. https://fiji.sc ilastik Berg et al.58 https://www.ilastik.org Imaris Oxford Instruments https://imaris.oxinst.com Moldex3D CoreTech System https://www.moldex3d.com MATLAB 2023B MathWorks https://se.mathworks.com/products/matlab.html measurePSF Rob Campbell https://github.com/SWC-Advanced- Microscopy/measurePSF BioRender BioRender https://biorender.com Image segmentation networks Deep learning Toolbox for resnet50 from MathWorks https://se.mathworks.com/help/ deeplearning/ref/resnet50.html Cell detection scripts for microwell image data This paper https://zenodo.org/records/14507473 https://doi.org/10.5281/zenodo.14507473 OPEN ACCESS

    Cell Isolation:

    Article Title: A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD11a (LFA-1) BioLegend Cat#301202; clone HI111; RRID: AB_314140 Mouse anti-human CD16 BioLegend Cat#302002; clone 3G8; RRID: AB_314202 Mouse anti-human Granzyme B – Alexa Fluor 647 BD Biosciences Cat#560212; clone GB11; RRID: AB_11154033 Mouse anti-human Vimentin – Alexa Fluor 488 Abcam Cat#ab195877 Goat anti-rabbit – Alexa Fluor 488 Abcam Cat#ab150089 Rabbit anti-human PKC-Q Santa Cruz Biotechnology Cat#sc-212; polyclonal C-18 Bacterial and virus strains LeGO-G2-puro https://www.lentigo-vectors. de/vectors.htm LeGO-G2/Puro pMDLg/pRRE Dull et al.54 Addgene #12251 pRSV-Rev Dull et al.54 Addgene #12253 pCMV-VSV-G Stewart et al.55 Addgene #8454 pLenti-CMV-MCS-RFP-SV-puro Jonathan Garlick and Behzad Gerami-Naini Addgene #109377 pLJM1-EGFP Sancak et al.56 Addgene #19319 pJML1-NES-LQDT-mGFP-P2A-NESVGPD-mCherry Beaudouin et al.57 N/A Biological samples Fresh buffy coats from healthy donors Karolinska University Hospital N/A Chemicals, peptides, and recombinant proteins Calcium Phosphate Transfection Kit Sigma-Aldrich CAPHOS Chloroquine Sigma-Aldrich Cat#C6628 Protamine sulfate Sigma-Aldrich Cat#P3369 Puromycin ThermoFisher Scientific Cat#A1113803 Interleukin-2 PeproTech Cat#200-02 Interleukin-15 R&D Systems Cat#247-ILB-005 D-Glucose Sigma-Aldrich Cat#D8375 D-Mannitol Sigma-Aldrich Cat#M4125 L-glutamine Sigma-Aldrich Cat#G7513 Lactic acid Sigma-Aldrich Cat#L6402 Anti-adherence rinsing solution STEMCELL Technologies Cat#07010 BD Pharmingen DRAQ5 BD Biosciences Cat#564902 Abberior LIVE 590 Tubulin Abberior LV590-0141 Phalloidin-STAR Red Abberior STRED-0100 CellTrace Yellow ThermoFisher Scientific Cat#C34567 Cytofix/Cytoperm BD Biosciences Cat#554714 Cytofix BD Biosciences Cat#554655 Perm/Wash BD Bioscences Cat#554714 Histodenz/Omnipaque (iohexol) Apoteket Cat#582692 Critical commercial assays TetraSpeck fluorescent microspheres kit Invitrogen Cat#T7284 Lenti-X Concentrator Takara Cat#631231 EasySep Human NK Cell Isolation Kit STEMCELL Technologies Cat#17955 (Continued on next page) e1 Cell Reports Methods 5, 100965, January 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Human: A498 cell line ATCC HTB-44 Human: OVCAR-8 cell line Gift from Brinton Seashore-Ludlow (Karolinska Institutet, Stockholm, Sweden) N/A Human: DLD-1 cell line ATCC CCL-221 Human: ACHN cell line ATCC CRL-1611 Human: K562 cell line ATCC CCL-243 Human: Jurkat cell line ATCC TIB-152 Human: Nalm-6 cell line DSMZ ACC 128 Human: SUP-B15 cell line Gift from Rozbeh Jafari (Karolinska Institutet, Stockholm, Sweden) N/A Human: Kasumi-1 cell line ATCC CRL-2724 Human: MCF-7 cell line ATCC HTB-22 Human: HeLa cell line ATCC CCL-2 Human: IMR-32 cell line Gift from Jakob Stenman (Karolinska Institutet, Stockholm) N/A Human: A549.Histone.mKate2 cell line Gift from Kalle Malmberg (Oslo University Hospital, Oslo, Norway) N/A Software and algorithms Prism GraphPad https://graphpad.com ImageJ Schneider et al. https://fiji.sc ilastik Berg et al.58 https://www.ilastik.org Imaris Oxford Instruments https://imaris.oxinst.com Moldex3D CoreTech System https://www.moldex3d.com MATLAB 2023B MathWorks https://se.mathworks.com/products/matlab.html measurePSF Rob Campbell https://github.com/SWC-Advanced- Microscopy/measurePSF BioRender BioRender https://biorender.com Image segmentation networks Deep learning Toolbox for resnet50 from MathWorks https://se.mathworks.com/help/ deeplearning/ref/resnet50.html Cell detection scripts for microwell image data This paper https://zenodo.org/records/14507473 https://doi.org/10.5281/zenodo.14507473 OPEN ACCESS



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