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dapi nuclear staining  (Beyotime)


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    Structured Review

    Beyotime dapi nuclear staining
    Dapi Nuclear Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+staining+with+dapi/DAPI/pm41981622-177-9-12
    Average 99 stars, based on 32584 article reviews
    dapi nuclear staining - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination
    Article Snippet: .. Cell staining and viability assays were performed using a LIVE/DEAD kit (calcein AM/propidium iodide), an annexin V–FITC/PI apoptosis detection kit, MTT reagent, Alexa Fluor 488–conjugated secondary antibody, and DAPI (Beyotime Biotechnology, Shanghai, China). .. The JC-1 MitoMP Detection Kit was obtained from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

    Article Title: CD52+ cells are associated with faster short-term neutrophil recovery in mobilized hematopoietic stem cell transplant.
    Article Snippet: .. Cells were stained with CD52 primary antibody (Bioss, #bs-34117R) at 4 oC for overnight, and subsequently incubated for 2 hours at 37 oC with the Alexa Flur 488- conjugated anti-rabbit secondary antibody (Invitrogen, #A21206) and DAPI (Beyotime Biotechnology, #P0131) were used for depiction of the nuclei. .. Image acquisition was performed using either a confocal ultra-spectral microscope (Zeiss, LSM900).

    Article Title: DEAD-box RNA helicase 21 interacts with the nucleocapsid protein and inhibits porcine deltacoronavirus infection through upregulation of interferon responses.
    Article Snippet: The cells were then incubated with primary antibodies for 1 h, followed by washing with PBS and incubation with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at 37°C in the dark. .. Afterward, the cells were stained with DAPI (Cat. No. C1005, Beyotime), mounted with glycerin, and observed under a confocal microscope (Olympus). ..

    MTT Assay:

    Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination
    Article Snippet: .. Cell staining and viability assays were performed using a LIVE/DEAD kit (calcein AM/propidium iodide), an annexin V–FITC/PI apoptosis detection kit, MTT reagent, Alexa Fluor 488–conjugated secondary antibody, and DAPI (Beyotime Biotechnology, Shanghai, China). .. The JC-1 MitoMP Detection Kit was obtained from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

    Incubation:

    Article Title: CD52+ cells are associated with faster short-term neutrophil recovery in mobilized hematopoietic stem cell transplant.
    Article Snippet: .. Cells were stained with CD52 primary antibody (Bioss, #bs-34117R) at 4 oC for overnight, and subsequently incubated for 2 hours at 37 oC with the Alexa Flur 488- conjugated anti-rabbit secondary antibody (Invitrogen, #A21206) and DAPI (Beyotime Biotechnology, #P0131) were used for depiction of the nuclei. .. Image acquisition was performed using either a confocal ultra-spectral microscope (Zeiss, LSM900).

    Article Title: Glucocorticoid-induced METTL3 promotes SONFH by enhancing MEG3 m6A methylation and subsequent ABL2 upregulation.
    Article Snippet: Background: Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory orthopedic disease.. While N6-methyladenosine (m6A) modification is implicated in bone metabolism, its specific role and mechanism in SONFH pathogenesis require further elucidation.. Methods: Bioinformatics analysis identified key m6A-related genes.

    TUNEL Assay:

    Article Title: Glucocorticoid-induced METTL3 promotes SONFH by enhancing MEG3 m6A methylation and subsequent ABL2 upregulation.
    Article Snippet: Background: Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory orthopedic disease.. While N6-methyladenosine (m6A) modification is implicated in bone metabolism, its specific role and mechanism in SONFH pathogenesis require further elucidation.. Methods: Bioinformatics analysis identified key m6A-related genes.

    Confocal:

    Article Title: DEAD-box RNA helicase 21 interacts with the nucleocapsid protein and inhibits porcine deltacoronavirus infection through upregulation of interferon responses.
    Article Snippet: The cells were then incubated with primary antibodies for 1 h, followed by washing with PBS and incubation with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at 37°C in the dark. .. Afterward, the cells were stained with DAPI (Cat. No. C1005, Beyotime), mounted with glycerin, and observed under a confocal microscope (Olympus). ..

    Microscopy:

    Article Title: DEAD-box RNA helicase 21 interacts with the nucleocapsid protein and inhibits porcine deltacoronavirus infection through upregulation of interferon responses.
    Article Snippet: The cells were then incubated with primary antibodies for 1 h, followed by washing with PBS and incubation with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at 37°C in the dark. .. Afterward, the cells were stained with DAPI (Cat. No. C1005, Beyotime), mounted with glycerin, and observed under a confocal microscope (Olympus). ..



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    Image Search Results


    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Journal: bioRxiv

    Article Title: Lymphatic vessel dysfunction contributes to severe dengue pathogenesis

    doi: 10.64898/2026.03.27.714698

    Figure Lengend Snippet: (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Article Snippet: The slides were then incubated with the nuclear stain DAPI (cat# D9542, Merck) at 1:1000 dilution for 10 min and mounted onto glass slides using mounting medium (InvitrogenTM Fluoromount-GTM Mounting Medium-00495802).

    Techniques: Cell Culture, Control, Staining