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sigmoidal nonlinear regression function  (GraphPad Software Inc)


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    GraphPad Software Inc sigmoidal nonlinear regression function
    Sigmoidal Nonlinear Regression Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonlinear+sigmoidal+regression+function/sigmoidal+nonlinear+regression+function/pm37060263-323-22-32
    Average 90 stars, based on 1 article reviews
    sigmoidal nonlinear regression function - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Deciphering the origin of total estrogenic activity of complex mixtures.
    Article Snippet: Estrogenic activity interpolation was calculated from specific dose-response available for each substance and their quantity using non-linear sigmoidal regression and the command interpolate unknowns from standard curve at confidence interval of 95% (GraphPad Software LLC, San Diego, CA, USA).

    Article Title: A broadly cross-reactive i-body to AMA1 potently inhibits blood and liver stages of Plasmodium parasites
    Article Snippet: Data was normalised and a sigmoidal non-linear regression was performed using GraphPad Prism 9.2 (GraphPad, USA).

    Article Title: A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases
    Article Snippet: After adjusting the absorbance for background and comparing it to the DMSO controls, the cytotoxic concentration CC 50 was calculated using a sigmoidal nonlinear regression function to fit the dose-response curve using the GraphPad Prism 9.

    Article Title: A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases.
    Article Snippet: After adjusting the absorbance for background and comparing it to the DMSO controls, the cytotoxic concentration CC50 was calculated using a sigmoidal nonlinear regression function to fit the dose-response curve using the GraphPad Prism 9.

    Software:

    Article Title: Chemical Fingerprinting, Aorta Endothelium Relaxation Effect, and Enzymatic Inhibition of Canelo ( Drimys winteri J. R. Forst. & G. Forst, (D.C) A. Gray, Family Winteraceae) Fruits.
    Article Snippet: .. In addition, the EC50 or IC50 determination was acquired using sigmoidal nonlinear regression by the Graph Pad Prism version 5.0 software package (GraphPad Software, Inc., La Jolla, CA, USA). ..

    Inhibition:

    Article Title: Macoligophones A-I, prenylated acetophenone dimers and monomers from Maclurodendron oligophlebium.
    Article Snippet: Chemical investigation of the bark of Maclurodendron oligophlebium resulted in the isolation of three prenylated acetophenone (PAP) dimers (1–3) and seventeen monomers (4–20).. Among them, macoligophones A–I (1, 2, and 4–10) are previously undescribed.. Utilizing chiral column, compounds 1–7 were separated into their individual enantiomers.

    Article Title: Stereochemical insights into enantioselective antiplasmodial lignanamides from the twigs and leaves of Solanum erianthum.
    Article Snippet: Stereochemical investigations on the twigs and leaves of Solanum erianthum afforded five pairs of lignanamide enantiomers and a previously undescribed phenolic amide (3).. Particularly, two pairs of previously undescribed lignanamide racemates (1a/1b–2a/2b) represent the first case of natural products that feature an unreported 5/ 5-fused N/O-biheterocyclic core.. Their structures, including the absolute configurations, were determined unambiguously by using spectroscopic analyses and electronic circular dichroism calculations.

    Control:

    Article Title: Macoligophones A-I, prenylated acetophenone dimers and monomers from Maclurodendron oligophlebium.
    Article Snippet: Chemical investigation of the bark of Maclurodendron oligophlebium resulted in the isolation of three prenylated acetophenone (PAP) dimers (1–3) and seventeen monomers (4–20).. Among them, macoligophones A–I (1, 2, and 4–10) are previously undescribed.. Utilizing chiral column, compounds 1–7 were separated into their individual enantiomers.

    Article Title: Stereochemical insights into enantioselective antiplasmodial lignanamides from the twigs and leaves of Solanum erianthum.
    Article Snippet: Stereochemical investigations on the twigs and leaves of Solanum erianthum afforded five pairs of lignanamide enantiomers and a previously undescribed phenolic amide (3).. Particularly, two pairs of previously undescribed lignanamide racemates (1a/1b–2a/2b) represent the first case of natural products that feature an unreported 5/ 5-fused N/O-biheterocyclic core.. Their structures, including the absolute configurations, were determined unambiguously by using spectroscopic analyses and electronic circular dichroism calculations.

    Concentration Assay:

    Article Title: Antioxidant Properties and Vasorelaxant Mechanism of Aqueous Extract of Ricinodendron heudelotii (Euphorbiaceae)
    Article Snippet: .. Analyses of concentration-response curves were performed by sigmoidal nonlinear regression (computer program: GraphPad Prism 5.00). ..



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    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a <t>Boltzmann</t> sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.
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    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a <t>Boltzmann</t> sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.
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    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a <t>Boltzmann</t> sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.
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    GraphPad Software Inc nonlinear regression (sigmoidal dose-response with variable slope) function
    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a <t>Boltzmann</t> sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.
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    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a Boltzmann sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.

    Journal: Science Advances

    Article Title: The immune-evasive proline-283 substitution in influenza nucleoprotein increases aggregation propensity without altering the native structure

    doi: 10.1126/sciadv.adl6144

    Figure Lengend Snippet: ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a Boltzmann sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.

    Article Snippet: For differential scanning fluorimetry, T agg values were obtained by performing a nonlinear regression to a Boltzmann sigmoid function using GraphPad Prism.

    Techniques: Circular Dichroism, Thermal Shift Assay, Expressing, Centrifugation, Western Blot