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nmda  (Tocris)


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    Structured Review

    Tocris nmda
    Nmda, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 2093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nmda/NMDA/pm41966427-137-20-21
    Average 96 stars, based on 2093 article reviews
    nmda - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: In utero exposure to NMDA receptor autoantibodies disrupts hippocampal circuit maturation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human IgG1 anti-NR1 (clone #003–102) Kreye et al. 49 N/A Human non-reactive IgG1 (clone #mGO53) Wardemann et al. 50 N/A Goat anti-human IgG (H + L) Cross-Absorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-21445; RRID: AB_2535862 mouse anti-human IgG (clone MT145) Mabtech RRID: AB_10697677 mouse anti-human IgG-ALP (clone MT78) Mabtech RRID: AB_10697678 Chemicals, peptides, and recombinant proteins DNQX Tocris Bioscience Cat#0189; Cas#2379-57-9 Gabazine (SR 95531 hydrobromide) Tocris Bioscience Cat#1262; Cas#104104-50-9 D-APV Tocris Bioscience Cat#0106; Cas#79055-68-8 DL-APV Tocris Bioscience Cat#0105; Cas#76326-31-3 DL-APV HelloBio Cat#HB0251; Cas#76326-31-3 Nifedipine Sigma-Aldrich Cat#N7634; Cas#21829-25-4 NMDA Tocris Bioscience Cat0114; Cas#6384-92-5 Tetrodotoxin citrate (TTX) Biotrend Cat# ARCD-0640-1; Cas#18660-81-6 Tetrodotoxin citrate (TTX) HelloBio Cat#HB1035; Cas#18660-81-6 Triton X-100 Sigma-Aldrich Cas#9036-19-5 2-methylbutane Merck KGaA Cas#78-78-4 DAPI Sigma-Aldrich Cas#28718-90-3 Fluoromount-G SouthernBiotech Cat#0100-01 Agarose Sigma-Aldrich Cas#9012-36-6 Normal donkey serum (NDS) Merck KGaA Cat#566460 Experimental models: Organisms/strains Mouse line (B6.129S2-Emx1 tm1(cre)Krj /J or Emx1 IREScre ) The Jackson Laboratory RRID: IMSR_JAX:005628 Mouse line (B6;129S6Gt(ROSA)26Sor tm96(CAG- GCaMP6s)Hze /J or GCaMP6s LSL ) The Jackson Laboratory RRID: IMSR_JAX:024106 Mouse line (C57BL/6J) The Jackson Laboratory RRID: IMSR_JAX:000664 Software and algorithms LabChart 8 ADInstruments RRID: SCR_023643 MES 8.3 Femtonics RRID: N/A MESc 3.5 Femtonics RRID: N/A ZEN 3.1 Carl Zeiss AG RRID: N/A pClamp (Clampfit) Molecular Devices RRID: SCR_011323 Patchmaster HEKA Elektronik RRID: SCR_000034 Mini Analysis Program Synaptosoft RRID: SCR_002184 OriginPro OriginLab RRID: SCR_014212 IBM SPSS Statistics IBM RRID: SCR_016479 MATLAB Mathworks RRID: SCR_001622 Fiji fiji.sc/ RRID: SCR_002285 ComDet package in Fiji imagej.net/plugins/spots-colocalization- comdet N/A (Continued on next page) Cell Reports 45, 117022, March 24, 2026 15 ..

    Membrane:

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons.
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of -60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of −60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Patch Clamp:

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons.
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of -60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..

    Article Title: GluN2A-mediated currents and calcium signal in human iPSC-derived neurons
    Article Snippet: .. Membrane currents elicited by NMDA (TOCRIS, HB0454) and Glycine (Millipore Sigma, 106-57-0) were recorded at a membrane potential of −60 mV using the whole-cell configuration of the patch-clamp technique with a List Electronics EPC-7 amplifier. ..



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    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or <t>NMDA</t> (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.
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    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or <t>NMDA</t> (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.
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    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or <t>NMDA</t> (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.
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    Image Search Results


    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or NMDA (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.

    Journal: Biosensors

    Article Title: Electrochemical Detection of Neuronal Injury in Cell Culture Samples: A Cost-Effective Biosensor for Neurofilament Light Sensing

    doi: 10.3390/bios16040212

    Figure Lengend Snippet: ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or NMDA (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.

    Article Snippet: N-methyl-D-aspartate (NMDA) was obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Immunofluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture