glun2b (Alomone Labs)
Structured Review

Glun2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nmda+receptor/Anti-NMDAR2B+(GluN2B)+(extracellular)+Antibody/pmc08050278-176-53-55
Average 96 stars, based on 126 article reviews
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1) Product Images from "Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells"
Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells
Journal: Scientific Reports
doi: 10.1038/s41598-021-87667-0
Figure Legend Snippet: Functional NMDA receptors are localized on the surface of HPASMCs. Immunofluorescence staining was performed on non-permeabilized HPASMCs. Both GluN1 and GluN2 (2B and 2D) subunits were detected on the surface of HPASMCs ( A , B ). GluN1 co-localized with GluN2B ( A ) and GluN2D ( B ) on the surface of HPASMCs, respectively. Results are representative images taken from at least three separate experiments. Scale bar = 100 µM. ( C , D ) are representative scatter plots of each fluorescent pixel from confocal images with GluN1 green fluorescent intensity along the x -axis and GluN2 red fluorescent intensity along the y -axis. The shaded area in the upper right quadrant represents colocalized pixels above background with the associated Pearson correlation coefficient indicated for all colocalized pixels. ( E ) Representative traces for cultured HPASMCs in response to 100 μM NMDA and 10 μM glycine treatment. Cells were clamped at − 50 mV and whole cell recordings were performed. ( F ) NMDA and glycine treatment evoked an inward whole-cell current of 10.9 ± 1.7 pA (Mean ± SE, **p < 0.01, n = 16). ( G ) NMDA and glycine treatment evoked increased AUC compared with baseline (*p < 0.05, n = 16).
Techniques Used: Functional Assay, Immunofluorescence, Staining, Cell Culture
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