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Shanghai GenePharma empty negative control (nc) plasmid
Empty Negative Control (Nc) Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+empty+plasmid/negative+control++nc++plasmids/pm39695074-94-6-17
Average 90 stars, based on 1 article reviews
empty negative control (nc) plasmid - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Negative Control:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Construct:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Knockdown:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Synthesized:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Over Expression:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Control:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

shRNA:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).

Transfection:

Article Title: Downregulation of tRNA methyltransferase FTSJ1 by PM2.5 promotes glycolysis and malignancy of NSCLC via facilitating PGK1 expression and translation.
Article Snippet: FTSJ1-overexpression (OE) plasmid and the empty negative control (NC) were constructed using the pcDNA3.1 (+) vectors by GenePharma (Suzhou, China). siRNAs for knockdown of FTSJ1 and siRNA-NC were designed and synthesized by GenePharma (Suzhou, China) too.

Article Title: ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.
Article Snippet: Funding information Key Discipline Construction Project of Shanghai Pudong New Area Health Commission, Grant/Award Number: PWZxk2022‐30; Science and Technology Development Fund for Livelihood Research (Medical and Health) in Pudong New Area, Shanghai, Grant/Award Number: PKJ2021‐Y23 Abstract Endoplasmic reticulum (ER) stress is a primary mechanism leading to cell apoptosis, making it of great research interests in cancer management.. This study delves into the function of ribosomal protein L5 (RPL5) in ER stress within pancreatic cancer (PCa) cells and investigates its regulatory mechanisms.. Bioinformatics predictions pinpointed RPL5 as an ER stress‐related gene exhibiting diminished expression in PCa.

Article Title: Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4-mediated NF-κB signaling pathway.
Article Snippet: For the transfection, the NOX4 overexpression plasmid and the negative control (NC) plasmid were purchased from GenePharma.

Article Title: Polyphyllin VII alleviates pulmonary hypertension by inducing miR-205-5p to target the β-catenin pathway.
Article Snippet: The miR-205–5p mimic/inhibitor, β-catenin pcDNA3.1 plasmid, along with corresponding negetive control (NC) plasmids were obtained from GenePharma (Shanghai, China).

Article Title: Propofol Regulates the TLR4/NF-κB Pathway Through miRNA-155 to Protect Colorectal Cancer Intestinal Barrier.
Article Snippet: Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal barrier and lead to bacterial invasion.. This study mainly analyzed whether propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC surgery, and explored its molecular mechanism.. A mouse CRC model was constructed using azomethane and dextran sulfate sodium.

Article Title: Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues.
Article Snippet: Overexpression plasmid of Sema3A (oe-Sema3A) and the negative control (NC) plasmid were provided by GenePharma Co., Ltd. (Shanghai, China).

Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
Article Snippet: An ACP5 gene overexpression plasmid and negative control (NC) plasmid were purchased from Genepharma Corporation (Shanghai, China).



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Cyagen Biosciences negative control hek293 cells transfected with an empty plasmid (nc-hek293)
Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The <t>transfection</t> efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.
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Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The transfection efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway

doi:

Figure Lengend Snippet: Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The transfection efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Western Blot, Knockdown, CCK-8 Assay, EdU Assay, Flow Cytometry

ELF1 interferes with cell proliferation and apoptosis by targeting DCLK1. The ELF1 overexpression plasmid and si-DCLK1 were co-transfected into Capan-2 cells, and the si-ELF1 and DCLC1 overexpression plasmids were co-transfected into PANC-1 cells. A. DCLK1 mRNA expression was probed by qRT-PCR after transfection. B, C. The viability of Capan-2 and PANC-1 cells was detected by CCK-8 and EdU assays. D. The apoptosis of Capan-2 and PANC-1 cells was detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway

doi:

Figure Lengend Snippet: ELF1 interferes with cell proliferation and apoptosis by targeting DCLK1. The ELF1 overexpression plasmid and si-DCLK1 were co-transfected into Capan-2 cells, and the si-ELF1 and DCLC1 overexpression plasmids were co-transfected into PANC-1 cells. A. DCLK1 mRNA expression was probed by qRT-PCR after transfection. B, C. The viability of Capan-2 and PANC-1 cells was detected by CCK-8 and EdU assays. D. The apoptosis of Capan-2 and PANC-1 cells was detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China).

Techniques: Over Expression, Plasmid Preparation, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry