nebnext end repair module (New England Biolabs)

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New England Biolabs nebnext end repair module
Nebnext End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+quick+ligation+module/NEBNext+Quick+Ligation+Module/pm42012196-217-12-16
Average 99 stars, based on 1073 article reviews

nebnext end repair module - by Bioz Stars, 2026-10

99/100 stars

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Sequencing:

Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

Article Title: Whole-Genome Long-Read Sequencing for Rapid Comprehensive Molecular Diagnostics of Brain Tumors
Article Snippet: Genomic DNA was extracted from fresh or frozen biopsies using the DNeasy Blood & Tissue kit (Qiagen). .. Sequencing libraries were prepared with the NEBNext companion module (New England Biolabs) and ligation sequencing kit (SQK-LSK114, ONT). ..

Article Title: Unraveling the potential of short and long read sequencing for human genome profiling
Article Snippet: .. We prepared the ONT library from 1.0 μg gDNA input (with no short read eliminator treatment), sheared using Megaruptor 3 (Diagenode) at speed 33 in a volume of 100 μL, using Ligation Sequencing kit V14 (ONT) and NEBNext Companion Module for ONT Ligation Sequencing (NEB), with clean-up steps using AMPure XP Beads (included with the ONT Ligation kit), all according to the manufacturers’ instructions. .. We performed QC using Qubit dsDNA HS assay kit (Thermo Fisher).

Ligation:

Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates
Article Snippet: NEBNext® Ultra II End repair/dA-tailing Module , New England BioLabs , E7546S. .. NEBNext® Quick Ligation Module , New England BioLabs , E6056S. .. Oxford Nanopore Technologies Ligation Sequencing Kit , Oxford Nanopore Technologies , SQK-LSK109.

Article Title: Genome‐Scale Engineering Importing Property of Escherichia coli for Improving Production of 3‐Hydroxypropionic Acid
Article Snippet: Fragmented DNA of 3–5 kb was purified via gel extraction using a GeneAll Expin Gel SV kit (GeneAll Biotechnology, Seoul, Korea). .. DNA fragment ends were repaired and phosphorylated with the NEBNext R End Repair Module kit (NEB, Ipswich, MA, USA), followed by ligation to a vector fragment amplified from pMD20 using pMD20_F and pMD20_R primers. .. The ligated products were introduced into ElectroMAX DH10B cells (NEB, Ipswich, MA, USA) to enhance transformation efficiency.

Amplification:

other:

Article Title: A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation.
Article Snippet: A 1X AMPure XP bead (Beckman Coulter, A63881) cleanup was performed before dephosphorylation of ends followed by second digestion with the inner guides M2 and P2 (24 μL eluate, 3 μL Quick CIP (NEB, M0525S), and 3 μL 10X rCutSmart buffer (NEB, B6004S) incubated at 37°C for 10 min and heat inactivated at 80°C for 2 min, followed by addition of 10 μL M2+P2 Cas9 RNP and incubation at 37°C for 1 hour).

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