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resource source identifier nebnext multiplexed oligos for illumina neb e7600s quick dna mini prep kit zymo d4068  (New England Biolabs)


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    New England Biolabs resource source identifier nebnext multiplexed oligos for illumina neb e7600s quick dna mini prep kit zymo d4068
    Resource Source Identifier Nebnext Multiplexed Oligos For Illumina Neb E7600s Quick Dna Mini Prep Kit Zymo D4068, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+identifier/NEBNext+Multi+Olig+IL+DualIndexPrim+Set1/pm41961593-197-2-5
    Average 96 stars, based on 2195 article reviews
    resource source identifier nebnext multiplexed oligos for illumina neb e7600s quick dna mini prep kit zymo d4068 - by Bioz Stars, 2026-11
    96/100 stars

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    Multiplex Assay:

    Article Title: Patient-Derived Fibroblasts as a Clinically Relevant Model of Kearns-Sayre Syndrome.
    Article Snippet: Amplicons were quantified using Qubit 2.0 (Invitrogen), and 1,000 ng were fragmented using NEBNext dsDNA Fragmentase (New England BioLabs) to fragments of approximately 250 bp and cleaned up with Ampure XP magnetic beads (Beckman Coulter). .. Samples were indexed and multiplexed using NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1; New England BioLabs) following the manufacturer’s instructions. .. Samples were pooled and loaded into MiSeq Reagent Kit v2 500-cycles (Illumina), and sequencing was carried out in MiSeq System (Illumina) with a theorical medium coverage of 10,000 reads.

    Article Title: A metabolic-epigenetic switch governs multicellular cardiac repair following succinate dehydrogenase inhibition.
    Article Snippet: DNA was purified (Qiagen MinElute PCR purification kit, 28006) and quantified by Qubit; fragment size was assessed using an Agilent 2200 TapeStation (high-sensitivity D1000 ScreenTape). .. Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, E7645L) with NEBNext multiplex oligos for Illumina (dual index primers set 1; New England Biolabs, E7600S), cleaned up with AMPure XP (Beckman Coulter, A63881) and sequenced on the Element Biosciences AVITI platform (2 × 75 bp, paired-end reads, ~10 million reads per sample). .. Antibodies used were as follows: rabbit anti-H3K4me3 (Cell Signaling Technology, 9751, 1:50), IgG XP isotype control (Cell Signaling Technology, 66362, 1:20) and rabbit anti-H3K27me3 (Cell Signaling Technology, 9733, 1:50).

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses
    Article Snippet: NEBNext Ultra II Ligation Module kit , NEB , E7595. .. NEBNext Multiplex Oligos for Illum , NEB , E7600. .. End Prep library kit , NEB , E7546.

    Article Title: Airborne Bacteria Are Influenced by Surrounding Soil, Litter and Vegetation at a Forest Campsite.
    Article Snippet: The PCR product (~300 bp) was purified with AMPure XP (Beckman Coulter). .. Illumina adapters and indices were then added with an NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) together with the NEBNext multiplex Oligos for Illumina (Dual Index Primers Set 1; NEB) following the manufacturer's protocol. ..

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Reactions were incubated at 16 °C for 1 h. The dsDNA was cleaned by the addition of 1.8× volumes of Mag-Bind TotalPure NGS beads (Omega Biotek), mixing, incubating 5 min RT, removing the unbound fraction, washing undisturbed beads with 200 μL of 80% ethanol twice, removing the ethanol and allowing evaporation of the residual liquid, followed by elution of the DNA in 50 μL of water. .. Library preparation and sequencing: The DNA was prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L, NEB) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S, NEB) per manufacturer’s protocol (with a 1–10 dilution of the standard adapter) except the blue capped USER enzyme (E7428AA, NEB) was used instead of red capped USER enzyme (E7602AVIAL, NEB), Mag-Bind TotalPure NGS beads (Omega Biotek) were used instead of NEBNext Sample Purification Beads, and 14 cycles of amplification were performed at the PCR stage. .. Pooled reactions were sequenced using NextSeq 1000 Sequencing System (Illumina) using a P2 100 cycle kit with 75 bp read 1, 25 bp read 2, 19 bp index 1, and 8 bp index 2 reads.

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Article Title: Albumin Hydrogels Loading Bacteriophage PA57 as a Promising Platform for Pseudomonas aeruginosa Infection Management.
    Article Snippet: .. A paired-end sequencing library was prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and Multiplex Oligos for Illumina Dual Index Primers Set 1 (New England Biolabs, Inc., Ipswich, MA, USA) for further sequencing on the MiSeq sequencer with a MiSeq reagent kit v.2 (2 × 250-cycles) (Illumina, San Diego, CA, USA). ..

    other:

    Article Title: Uncovering essential RNA components of fusion oncoprotein condensates using bicon-seq.
    Article Snippet: Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models dTAG-V5-NUP98::KDM5A Troester et al (2025) NA dTAG-GFP-V5-NUP98::KDM5A Troester et al (2025) NA GFP-V5-NUP98::KDM5A Zhang et al (2025) NA DDX6-dTAG MOLM13 Kodali et al (2024) NA LentiX 293T cells Takara bio 632180 U2OS ATCC HTB-96 Recombinant DNA LeGO-lnc-MONC Addgene #86966 LeGO-lnc-Gas5 This study NA pLenti-SFFV-dCas9-KRABMeCP2-P2A-Blasti Addgene #122205 psPAX2 Addgene #12260 pMD2.G Addgene #12259 Antibodies V5-Tag (D3H8Q) Rabbit mAb Cell Signaling Technology 13202S Rabbit IgG Diagenode C15410206 Mouse anti-CD11b/Mac-1 BV 421 (clone M1/70) BioLegend 101235 Mouse anti-Gr-1/Ly-6C PE-Cy7 (clone RB6-8C5) BioLegend 108415 Mouse anti-CD117/c-Kit APC (clone 2B8) BioLegend 105811 Anti-GFP Abcam ab290 Normal Rabbit IgG Cell Signaling Technology 2729 Rabbit anti-DDX6 Novus Biologicals NB200-192 Mouse anti-G3BP1 (F6X8V) Cell Signaling Technology 48372 Rabbit anti-PSPC1 (E7L8B) Cell Signaling Technology 65992 Rabbit anti-NPM1 Abcam ab15440 Rabbit anti-Coilin Abcam ab210785 Rabbit anti-TRAP220/MED1 Abcam ab64965 F(ab’)2-Goat anti-Rabbit IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen A-21246 14 The EMBO Journal © The Author(s) Reagent/Resource Reference or Source Identifier or Catalog Number Goat anti-Mouse IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen A-21235 Oligonucleotides and other sequence-based reagents PCR primers This study Dataset EV1 Chemicals, enzymes and other reagents Biotinylated isoxazole Sigma-Aldrich 900572 7-AAD Viability Staining Solution Invitrogen 00-6993-42 Annexin V Pacific Blue conjugate Invitrogen #A35122 Sodium arsenite Sigma-Aldrich S7400 DMSO Sigma-Aldrich D4540 dTAG-13 Sigma-Aldrich SML2601 DMEM, high glucose, no glutamine Gibco, Thermo Fisher Scientific 11510416 IMDM Gibco, Thermo Fisher Scientific 11504556 FBS Sigma-Aldrich F9665 Penicillin-Streptomycin Gibco, Thermo Fisher Scientific 11548876 L-Glutamine Gibco, Thermo Fisher Scientific 11500626 2-Mercaptoethanol Gibco, Fisher Scientific 11528926 SCF PeproTech, Thermo Fisher Scientific 250-03-10UG mIL-3 PeproTech, Thermo Fisher Scientific 213-13-10UG mIL-6 PeproTech, Thermo Fisher Scientific 216-16-10UG RPMI 1640 Gibco, Thermo Fisher Scientific 11544446 HEPES Sigma-Aldrich H0887 Sodium pyruvate Sigma-Aldrich S8636 DMEM, high glucose, GlutaMAX Supplement Gibco, Thermo Fisher Scientific 10566016 Polybrene Szabo-Scandic SACSC134220A 0.45 μM filter TPP TPP-99745 NaCl Carl Roth HN00.3 NP-40 alternative Millipore 492016 EDTA Sigma-Aldrich E9884 EGTA Millipore 324626 Glycerol Thermo Scientific Chemicals 158920025 Protease Inhibitor Cocktail Roche 11697498001 NaF Sigma-Aldrich 201154 PMSF Roche 10837091001 DTT Thermo Scientific A39255 TPCK Sigma-Aldrich T4376 Reagent/Resource Reference or Source Identifier or Catalog Number Pierce Bradford Protein Assay Kit Thermo Scientific 23200 NEB Monarch Total RNA Miniprep Kit NEB T1110 QuantSeq 3‘ mRNA-Seq Library Prep Kit Lexogen 015.96 Tris-HCl ITW reagents,AppliChem A1087,1000 MgCl2 Sigma-Aldrich M8266 Vanadyl Ribonucleoside Complex NEB S1402S RNA ChIP-IT Magnetic Chromatin Immunoprecipitation Kit Active Motif Cat#53024 Formaldehyde Sigma-Aldrich 252549 Trizol LS Reagent Invitrogen 10296010 Chloroform Merck 102447 Isopropanol Merck 190764 Linear acrylamide Thermo Scientific Chemicals J67830-XF RevertAid First Strand cDNA Synthesis Kit Thermo Scientific K1621 SsoAdvanced Universal SYBR Green Supermix Bio-Rad #1725271 RNAscope Multiplex Fluorescent v2 Assay ACD Bio-Techne 323100 Histofix Roth P087.6 Ethanol Sigma-Aldrich 51976 RNAscope probe-Mm-Gas5 Tocris Bioscience #561631 RNAscope probe-Mm-Malat1 Tocris Bioscience #313391 RNAscope negative control probe Tocris Bioscience #320871 TSA-Cy3 Tocris Bioscience #7527 Polyethylenimine Merck 408727 Spermidine Sigma-Aldrich S2626 Digitonin Sigma-Aldrich D141 BioMagPlus Concanavalin A Polysciences 86057 RNase A (DNase-free) ITW Reagents A3832,0050 AmPure XP Beads Beckman Coulter A63880 NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs E7645S NEBNext Multiplex Oligos for Illumina (Dual Index Set 1) New England Biolabs E7600S Triton X-100 Sigma-Aldrich X100 cOmplete, EDTA-free Protease Inhibitor Cocktail Roche 04693132001 pAG-Mnase In-house NA Monarch PCR & DNA Cleanup Kit New England Biolabs T1030 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies 11-05-01-15 Illumina Tagment DNA Enzyme and Buffer Small Kit Illumina 20034197 © The Author(s) The EMBO Journal 15 Reagent/Resource Reference or Source Identifier or Catalog Number Illumina DNA/RNA UD Indexes Set A, Tagmentation Illumina 20091654 NEBNext High-Fidelity 2X PCR master mix New England Biolabs M0541S MinElute PCR Purification Kit Qiagen 28004 SYBR Green I dye Invitrogen S7563 IGEPAL CA-630 Sigma-Aldrich I3021 Histofix Carl ROTH P087.6 Triton X-100 Sigma-Aldrich T8787 DPBS Gibco 14190169 RNAscope Protease III ACD Bio-Techne 322337 RNAscope Wash buffer ACD Bio-Techne 320058 GFP-Booster ATTO488 ChromoTek Gba488-100 Aqua-Poly/Mount antifade medium Polysciences 18606 Normal goat serum Invitrogen 31872 1.5H glass bottoms Ibidi 80827 (3-Mercaptopropyl) trimethoxysilane Sigma-Aldrich 175617 Mal-PEG3-NH2 TFA MedChemExpress YHW190796A Dibenzocyclooctyne-Nhydroxysuccinimidyl ester Sigma-Aldrich 761524 Ac4ManNAz TargetMol T14093 Attofluor Cell Chamber Invitrogen A7816 Software ForeCyte software Essen Bioscience, Ann Arbor, Michigan, USA NA GraphPad Prism 10 https://www.graphpad.

    Sequencing:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Reactions were incubated at 16 °C for 1 h. The dsDNA was cleaned by the addition of 1.8× volumes of Mag-Bind TotalPure NGS beads (Omega Biotek), mixing, incubating 5 min RT, removing the unbound fraction, washing undisturbed beads with 200 μL of 80% ethanol twice, removing the ethanol and allowing evaporation of the residual liquid, followed by elution of the DNA in 50 μL of water. .. Library preparation and sequencing: The DNA was prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L, NEB) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S, NEB) per manufacturer’s protocol (with a 1–10 dilution of the standard adapter) except the blue capped USER enzyme (E7428AA, NEB) was used instead of red capped USER enzyme (E7602AVIAL, NEB), Mag-Bind TotalPure NGS beads (Omega Biotek) were used instead of NEBNext Sample Purification Beads, and 14 cycles of amplification were performed at the PCR stage. .. Pooled reactions were sequenced using NextSeq 1000 Sequencing System (Illumina) using a P2 100 cycle kit with 75 bp read 1, 25 bp read 2, 19 bp index 1, and 8 bp index 2 reads.

    Article Title: Albumin Hydrogels Loading Bacteriophage PA57 as a Promising Platform for Pseudomonas aeruginosa Infection Management.
    Article Snippet: .. A paired-end sequencing library was prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and Multiplex Oligos for Illumina Dual Index Primers Set 1 (New England Biolabs, Inc., Ipswich, MA, USA) for further sequencing on the MiSeq sequencer with a MiSeq reagent kit v.2 (2 × 250-cycles) (Illumina, San Diego, CA, USA). ..

    Next-Generation Sequencing:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Reactions were incubated at 16 °C for 1 h. The dsDNA was cleaned by the addition of 1.8× volumes of Mag-Bind TotalPure NGS beads (Omega Biotek), mixing, incubating 5 min RT, removing the unbound fraction, washing undisturbed beads with 200 μL of 80% ethanol twice, removing the ethanol and allowing evaporation of the residual liquid, followed by elution of the DNA in 50 μL of water. .. Library preparation and sequencing: The DNA was prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L, NEB) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S, NEB) per manufacturer’s protocol (with a 1–10 dilution of the standard adapter) except the blue capped USER enzyme (E7428AA, NEB) was used instead of red capped USER enzyme (E7602AVIAL, NEB), Mag-Bind TotalPure NGS beads (Omega Biotek) were used instead of NEBNext Sample Purification Beads, and 14 cycles of amplification were performed at the PCR stage. .. Pooled reactions were sequenced using NextSeq 1000 Sequencing System (Illumina) using a P2 100 cycle kit with 75 bp read 1, 25 bp read 2, 19 bp index 1, and 8 bp index 2 reads.

    Sample Purification:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Reactions were incubated at 16 °C for 1 h. The dsDNA was cleaned by the addition of 1.8× volumes of Mag-Bind TotalPure NGS beads (Omega Biotek), mixing, incubating 5 min RT, removing the unbound fraction, washing undisturbed beads with 200 μL of 80% ethanol twice, removing the ethanol and allowing evaporation of the residual liquid, followed by elution of the DNA in 50 μL of water. .. Library preparation and sequencing: The DNA was prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L, NEB) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S, NEB) per manufacturer’s protocol (with a 1–10 dilution of the standard adapter) except the blue capped USER enzyme (E7428AA, NEB) was used instead of red capped USER enzyme (E7602AVIAL, NEB), Mag-Bind TotalPure NGS beads (Omega Biotek) were used instead of NEBNext Sample Purification Beads, and 14 cycles of amplification were performed at the PCR stage. .. Pooled reactions were sequenced using NextSeq 1000 Sequencing System (Illumina) using a P2 100 cycle kit with 75 bp read 1, 25 bp read 2, 19 bp index 1, and 8 bp index 2 reads.

    Amplification:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Reactions were incubated at 16 °C for 1 h. The dsDNA was cleaned by the addition of 1.8× volumes of Mag-Bind TotalPure NGS beads (Omega Biotek), mixing, incubating 5 min RT, removing the unbound fraction, washing undisturbed beads with 200 μL of 80% ethanol twice, removing the ethanol and allowing evaporation of the residual liquid, followed by elution of the DNA in 50 μL of water. .. Library preparation and sequencing: The DNA was prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L, NEB) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S, NEB) per manufacturer’s protocol (with a 1–10 dilution of the standard adapter) except the blue capped USER enzyme (E7428AA, NEB) was used instead of red capped USER enzyme (E7602AVIAL, NEB), Mag-Bind TotalPure NGS beads (Omega Biotek) were used instead of NEBNext Sample Purification Beads, and 14 cycles of amplification were performed at the PCR stage. .. Pooled reactions were sequenced using NextSeq 1000 Sequencing System (Illumina) using a P2 100 cycle kit with 75 bp read 1, 25 bp read 2, 19 bp index 1, and 8 bp index 2 reads.

    Polymerase Chain Reaction:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Reactions were incubated at 16 °C for 1 h. The dsDNA was cleaned by the addition of 1.8× volumes of Mag-Bind TotalPure NGS beads (Omega Biotek), mixing, incubating 5 min RT, removing the unbound fraction, washing undisturbed beads with 200 μL of 80% ethanol twice, removing the ethanol and allowing evaporation of the residual liquid, followed by elution of the DNA in 50 μL of water. .. Library preparation and sequencing: The DNA was prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L, NEB) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S, NEB) per manufacturer’s protocol (with a 1–10 dilution of the standard adapter) except the blue capped USER enzyme (E7428AA, NEB) was used instead of red capped USER enzyme (E7602AVIAL, NEB), Mag-Bind TotalPure NGS beads (Omega Biotek) were used instead of NEBNext Sample Purification Beads, and 14 cycles of amplification were performed at the PCR stage. .. Pooled reactions were sequenced using NextSeq 1000 Sequencing System (Illumina) using a P2 100 cycle kit with 75 bp read 1, 25 bp read 2, 19 bp index 1, and 8 bp index 2 reads.

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Real-time Polymerase Chain Reaction:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    SYBR Green Assay:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Purification:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Chromatin Immunoprecipitation:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    RNA Sequencing:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Western Blot:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Fluorescence:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Microscopy:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).



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    Samples used as positive controls to validate the deep amplicon <t> sequencing </t> approach for the accurate detection of species composition.
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    resource source identifier  (New England Biolabs)
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    Samples used as positive controls to validate the deep amplicon <t> sequencing </t> approach for the accurate detection of species composition.
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    Samples used as positive controls to validate the deep amplicon <t> sequencing </t> approach for the accurate detection of species composition.
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    Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Amplification, Sequencing, Cell Culture

    Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing

    Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing