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multiplex identifier (mid) primer  (Thermo Fisher)


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    Thermo Fisher multiplex identifier (mid) primer
    Multiplex Identifier (Mid) Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+identifier/multiplex+identifier++mid++primer/pmc11597161-49-20-45
    Average 90 stars, based on 1 article reviews
    multiplex identifier (mid) primer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The Evolution of Wisteria Vein Mosaic Virus: A Case Study Approach to Track the Emergence of New Potyvirus Threats
    Article Snippet: The obtained cDNA was used as a template to perform a PCR-based random amplification in the presence of 1 μM multiplex identifier (MID) primer (5′- CAAGTCAA TGTGTTGGGTGTGTTTGG-3′, MID tag in bold), 0.25 mM DNTPs, 0.3 U of DyNAzymeTM II DNA polymerase and 1X reaction buffer (Thermo Fisher Scientific, Waltham, MA, USA).

    Amplification:

    Article Title: The Evolution of Wisteria Vein Mosaic Virus: A Case Study Approach to Track the Emergence of New Potyvirus Threats
    Article Snippet: The obtained cDNA was used as a template to perform a PCR-based random amplification in the presence of 1 μM multiplex identifier (MID) primer (5′- CAAGTCAA TGTGTTGGGTGTGTTTGG-3′, MID tag in bold), 0.25 mM DNTPs, 0.3 U of DyNAzymeTM II DNA polymerase and 1X reaction buffer (Thermo Fisher Scientific, Waltham, MA, USA).

    Multiplex Assay:

    Article Title: The Evolution of Wisteria Vein Mosaic Virus: A Case Study Approach to Track the Emergence of New Potyvirus Threats
    Article Snippet: The obtained cDNA was used as a template to perform a PCR-based random amplification in the presence of 1 μM multiplex identifier (MID) primer (5′- CAAGTCAA TGTGTTGGGTGTGTTTGG-3′, MID tag in bold), 0.25 mM DNTPs, 0.3 U of DyNAzymeTM II DNA polymerase and 1X reaction buffer (Thermo Fisher Scientific, Waltham, MA, USA).



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    Image Search Results


    Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Amplification, Sequencing, Cell Culture

    Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing

    Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing