resource source identifier nebnext multiplex oligos for illumina (New England Biolabs)
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resource source identifier nebnext multiplex oligos for illumina
Resource Source Identifier Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+identifier/NEBNext+Multiplex+Oligos+for+Illumina/pm38306273-269-2-5
Average 96 stars, based on 2195 article reviews
Resource Source Identifier Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+identifier/NEBNext+Multiplex+Oligos+for+Illumina/pm38306273-269-2-5
Average 96 stars, based on 2195 article reviews
resource source identifier nebnext multiplex oligos for illumina - by Bioz Stars,
2026-11
96/100 stars
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other:Article Title: Nucleosome fibre topology guides transcription factor binding to enhancers. Article Snippet: For cell cross-linking, 3 ml of formaldehyde cross-linking buffer (50 mM HEPES-KOH, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 11% formaldehyde) was added to 15 cm dishes (Corning, 430599) containing 30 ml medium and incubated at room temperature for 10 min with swirling every 2 min. Cross-linking was blocked by adding 1.65 ml 2.5 M glycine and incubating for 5 min with swirling at room temperature. Amplification:Article Title: Identification and genetic dissection of convergent persister cell states. Article Snippet: 12.5 μl NT (FC-131–1096, Illumina) was immediately added to stop the reaction. .. Tagmented cDNA was amplified in a 500 μl PCR with Q5 polymerase (M0491L, New England Biolabs): 100 μl 5× buffer, 10 μl 10 mM dNTPs (N0447L, New England Biolabs), 5 μl Q5 polymerase, 85 μl Q5 High GC Enhancer, 0.5 μM N70x (Nextera Index Kit v2 Set A, TG-131-2001, Illumina; or equivalent from Integrated DNA Technologies), 0.5 μM Article Title: Identification and genetic dissection of convergent persister cell states Article Snippet: 12.5 μl NT (FC-131–1096, Illumina) was immediately added to stop the reaction. .. Tagmented cDNA was amplified in a 500 μl PCR with Q5 polymerase (M0491L, New England Biolabs): 100 μl 5× buffer, 10 μl 10 mM dNTPs (N0447L, New England Biolabs), 5 μl Q5 polymerase, 85 μl Q5 High GC Enhancer, 0.5 μM N70x (Nextera Index Kit v2 Set A, TG-131-2001, Illumina; or equivalent from Integrated DNA Technologies), 0.5 μM Polymerase Chain Reaction:Article Title: Identification and genetic dissection of convergent persister cell states. Article Snippet: 12.5 μl NT (FC-131–1096, Illumina) was immediately added to stop the reaction. .. Tagmented cDNA was amplified in a 500 μl PCR with Q5 polymerase (M0491L, New England Biolabs): 100 μl 5× buffer, 10 μl 10 mM dNTPs (N0447L, New England Biolabs), 5 μl Q5 polymerase, 85 μl Q5 High GC Enhancer, 0.5 μM N70x (Nextera Index Kit v2 Set A, TG-131-2001, Illumina; or equivalent from Integrated DNA Technologies), 0.5 μM Article Title: Endogenous neuronal DNA double-strand breaks are not sufficient to drive brain aging and neurodegeneration Article Snippet: Transposition was performed in a 50-μL reaction containing 4.7 μL TDE1 Tagment DNA enzyme (Illumina, 20034198), and the reaction was cleaned up (DNA Clean and Concentrator kit; Zymo, D4034). .. NEBNext Ultra II master mix (NEB, M0544L), SYBR Green I (Thermo Fisher Scientific, S7563), and 0.5 μM of sample-specific Article Title: Epididymis-specific RNase A family genes regulate fertility and small RNA processing Article Snippet: The cDNA was purified using the MiniElute Reaction Cleanup kit and run on a precast 10% Urea PAGE gel (BioRad #4566033) for 45 min at 200V. cDNA corresponding to small RNAs, including miRNAs and full-length tRNAs, was cut from the gel using Cy5 imaging, and DNA gel extraction was performed. .. The extracted cDNA was used for PCR reaction for 12 cycles using Q5 Polymerase and Article Title: Nucleosome fibre topology guides transcription factor binding to enhancers. Article Snippet: Micrococcal nuclease (Worthington Biochemicals, LS004797) was added to a final concentration of 0, 1, 4, 16 or 64 U ml−1 and chromatin was digested for 2 min in a 37 °C water bath. .. The beads were then split into nine PCR tubes (to reduce the number of beads settling in individual PCR tubes during PCR cycling), and 10 μl NEBNext Ultra II Q5 Master Mix, 2 μl 10 μM NEBNext i5 primer, 2 μl 10 μM Article Title: Identification and genetic dissection of convergent persister cell states Article Snippet: 12.5 μl NT (FC-131–1096, Illumina) was immediately added to stop the reaction. .. Tagmented cDNA was amplified in a 500 μl PCR with Q5 polymerase (M0491L, New England Biolabs): 100 μl 5× buffer, 10 μl 10 mM dNTPs (N0447L, New England Biolabs), 5 μl Q5 polymerase, 85 μl Q5 High GC Enhancer, 0.5 μM N70x (Nextera Index Kit v2 Set A, TG-131-2001, Illumina; or equivalent from Integrated DNA Technologies), 0.5 μM Article Title: Epididymis-specific RNase A family genes regulate fertility and small RNA processing. Article Snippet: The cDNA was purified using the MiniElute Reaction Cleanup kit and run on a precast 10% Urea PAGE gel (BioRad #4566033) for 45 minutes at 200V. cDNA corresponding to small RNAs, including miRNAs and full-length tRNAs, was cut from the gel using Cy5 imaging, and DNA gel extraction was performed. .. The extracted cDNA was used for PCR reaction for 12 cycles using Q5 Polymerase and SYBR Green Assay:Article Title: Endogenous neuronal DNA double-strand breaks are not sufficient to drive brain aging and neurodegeneration Article Snippet: Transposition was performed in a 50-μL reaction containing 4.7 μL TDE1 Tagment DNA enzyme (Illumina, 20034198), and the reaction was cleaned up (DNA Clean and Concentrator kit; Zymo, D4034). .. NEBNext Ultra II master mix (NEB, M0544L), SYBR Green I (Thermo Fisher Scientific, S7563), and 0.5 μM of sample-specific Multiplex Assay:Article Title: Epididymis-specific RNase A family genes regulate fertility and small RNA processing Article Snippet: The cDNA was purified using the MiniElute Reaction Cleanup kit and run on a precast 10% Urea PAGE gel (BioRad #4566033) for 45 min at 200V. cDNA corresponding to small RNAs, including miRNAs and full-length tRNAs, was cut from the gel using Cy5 imaging, and DNA gel extraction was performed. .. The extracted cDNA was used for PCR reaction for 12 cycles using Q5 Polymerase and Article Title: Epididymis-specific RNase A family genes regulate fertility and small RNA processing. Article Snippet: The cDNA was purified using the MiniElute Reaction Cleanup kit and run on a precast 10% Urea PAGE gel (BioRad #4566033) for 45 minutes at 200V. cDNA corresponding to small RNAs, including miRNAs and full-length tRNAs, was cut from the gel using Cy5 imaging, and DNA gel extraction was performed. .. The extracted cDNA was used for PCR reaction for 12 cycles using Q5 Polymerase and Chromatin Immunoprecipitation:Article Title: Nucleosome fibre topology guides transcription factor binding to enhancers. Article Snippet: ChIP reactions were quantified by Qubit 2.0 using the HS dsDNA quantification kit (Thermo Fisher Scientific, Q32854). .. ChIP–seq DNA libraries were prepared using the NEBNext Ultra II Library Preparation Kit (NEB, E7645S) with |
