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sequence-optimized fragments  (GenScript corporation)

 
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    GenScript corporation sequence-optimized fragments
    Sequence Optimized Fragments, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multi-parameter+gene+optimization+algorithm%2C+optimumgenetm/sequence+optimized+fragments/pmc09731445-31-3-5
    Average 90 stars, based on 1 article reviews
    sequence-optimized fragments - by Bioz Stars, 2026-10
    90/100 stars

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    Construct:

    Article Title: Genus
    Article Snippet: The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The approximately 1.7-kb DNA fragment of the codon-optimized gene that was synthesized, and the above-mentioned DNA fragment of approximately 10.6-kb from cloning vector pCYK21 were each cleaved by using restriction enzymes NdeI and HindIII.

    Cloning:

    Article Title: Genus
    Article Snippet: The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The approximately 1.7-kb DNA fragment of the codon-optimized gene that was synthesized, and the above-mentioned DNA fragment of approximately 10.6-kb from cloning vector pCYK21 were each cleaved by using restriction enzymes NdeI and HindIII.

    Plasmid Preparation:

    Article Title: Genus
    Article Snippet: The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The approximately 1.7-kb DNA fragment of the codon-optimized gene that was synthesized, and the above-mentioned DNA fragment of approximately 10.6-kb from cloning vector pCYK21 were each cleaved by using restriction enzymes NdeI and HindIII.

    Gene Expression:

    Article Title: Genus
    Article Snippet: The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The approximately 1.7-kb DNA fragment of the codon-optimized gene that was synthesized, and the above-mentioned DNA fragment of approximately 10.6-kb from cloning vector pCYK21 were each cleaved by using restriction enzymes NdeI and HindIII.

    Sequencing:

    Article Title: Genus
    Article Snippet: The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The approximately 1.7-kb DNA fragment of the codon-optimized gene that was synthesized, and the above-mentioned DNA fragment of approximately 10.6-kb from cloning vector pCYK21 were each cleaved by using restriction enzymes NdeI and HindIII.

    Article Title: Anti-IL-17A antibody and use thereof
    Article Snippet: .. The heavy chain and light chain variable region sequence fragments were synthesized by GenScript. ..

    Article Title: Adenovirus and uses thereof
    Article Snippet: .. Sequence fragments comprising the respective modified hexon and fiber sequences were synthesized (by GenScript) and then subjected to sequential subcloning steps that together amounted to insertion of those sequences into pAd4.5orf6 (replacing therein the corresponding native HAdV-4 hexon- and fiber-comprising sequences). ..

    Synthesized:

    Article Title: Genus
    Article Snippet: The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The constructed cloning vector for gene expression was named pCYK21. (3) Transformant Having Isobutanol Producing Ability (3-1) Codon Optimization of the 2-Keto-Acid Decarboxylase Gene of Lactococcus lactis Codon usage of kivD gene encoding the 2-keto-acid decarboxylase of Lactococcus lactis was optimized according to the codon usage frequency of Hydrogenophilus thermoluteolus strain TH-1 (NBRC 14978), and the DNA fragment of the optimized base sequence (SEQ ID NO: 1) was synthesized by GenScript Japan Inc. .. The approximately 1.7-kb DNA fragment of the codon-optimized gene that was synthesized, and the above-mentioned DNA fragment of approximately 10.6-kb from cloning vector pCYK21 were each cleaved by using restriction enzymes NdeI and HindIII.

    Article Title: Anti-IL-17A antibody and use thereof
    Article Snippet: .. The heavy chain and light chain variable region sequence fragments were synthesized by GenScript. ..

    Article Title: Adenovirus and uses thereof
    Article Snippet: .. Sequence fragments comprising the respective modified hexon and fiber sequences were synthesized (by GenScript) and then subjected to sequential subcloning steps that together amounted to insertion of those sequences into pAd4.5orf6 (replacing therein the corresponding native HAdV-4 hexon- and fiber-comprising sequences). ..

    other:

    Article Title: Triggering of lymphocytes by CD28, 4-1BB, and PD-1 checkpoints to enhance the immune response capacities
    Article Snippet: These fragments were sequence-optimized by GenScript’s Multi-parameter Gene Optimization algorithm, OptimumGeneTM (NJ, USA), and were synthetized by Biomatik (Ontario, Canada).

    Article Title: Activation of T Lymphocytes with Anti-PDL1-BiTE in the Presence of Adipose-Derived Mesenchymal Stem Cells (ASCs)
    Article Snippet: These fragments were sequence-optimized by GenScript’s Multiparameter Gene Optimization algorithm, OptimumGeneTM (NJ, USA) and were synthetized by Biomatik (Ontario, Canada).

    Article Title: Triggering of lymphocytes by CD28, 4-1BB, and PD-1 checkpoints to enhance the immune response capacities.
    Article Snippet: These fragments were sequence-optimized by GenScript’s Multiparameter Gene Optimization algorithm, OptimumGeneTM (NJ, USA), and were synthetized by Biomatik (Ontario, Canada).

    Article Title: Activation of T Lymphocytes with Anti-PDL1-BiTE in the Presence of Adipose-Derived Mesenchymal Stem Cells (ASCs)
    Article Snippet: These fragments were sequence-optimized by GenScript's Multiparameter Gene Optimization algorithm, OptimumGeneTM (NJ, USA) and were synthetized by Biomatik (Ontario, Canada).

    Modification:

    Article Title: Adenovirus and uses thereof
    Article Snippet: .. Sequence fragments comprising the respective modified hexon and fiber sequences were synthesized (by GenScript) and then subjected to sequential subcloning steps that together amounted to insertion of those sequences into pAd4.5orf6 (replacing therein the corresponding native HAdV-4 hexon- and fiber-comprising sequences). ..

    Subcloning:

    Article Title: Adenovirus and uses thereof
    Article Snippet: .. Sequence fragments comprising the respective modified hexon and fiber sequences were synthesized (by GenScript) and then subjected to sequential subcloning steps that together amounted to insertion of those sequences into pAd4.5orf6 (replacing therein the corresponding native HAdV-4 hexon- and fiber-comprising sequences). ..



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