other:Article Title: Method for detecting opioids, opiates, cannabinoids, or benzodiazepines in a sample with a β-glucuronidase enzyme
Article Snippet: To express the β-glucuronidase enzyme, nucleotide sequence from said protein was used, originated from Brachyspira pilosicoli strain B2904 described in Genbank (access code CP003490), which is shown in SEQ ID NO:2, and codon optimization was performed using the algorithm OptimumGeneTM (GenScript) to efficiently express said sequence in E. coli.
Article Title: LucID: A Self-Activating Bioluminescent Biotin Ligase for Light-Free Proximity Labeling in Deep Tissues
Article Snippet: Tool used : OptimumGeneTM (GenScript) Parameters : Host organism: Homo sapiens Removal of common restriction sites: EcoRI, XhoI, BamHI CAI (Codon Adaptation Index) improved from 0.92 to 0.98 GC content reduced from 52% to 45% to improve mRNA stability and translation efficiency
Article Title: Compositions and methods for genome editing in plants
Article Snippet: Programs for codon optimization are available in the art (e.g., OPTIMIZER at genomes.urv.es/OPTIMIZER; OptimumGeneTM from GenScript at genscript.com/codon_opt.html).
Software:Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer.
Article Snippet: In brief, we commercially synthesized a bsh (efpBSH) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGeneTM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli (https://www.genscript.com/gensmartfree-gene-codon-optimization.html) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD600 value reached 0.5–0.6, the culture medium was statically incubated at 15 ◦C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer
Article Snippet: In brief, we commercially synthesized a bsh ( efpBSH ) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGene TM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli ( https://www.genscript.com/gensmart-free-gene-codon-optimization.html ) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD 600 value reached 0.5–0.6, the culture medium was statically incubated at 15 °C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Gene Expression:Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer.
Article Snippet: In brief, we commercially synthesized a bsh (efpBSH) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGeneTM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli (https://www.genscript.com/gensmartfree-gene-codon-optimization.html) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD600 value reached 0.5–0.6, the culture medium was statically incubated at 15 ◦C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer
Article Snippet: In brief, we commercially synthesized a bsh ( efpBSH ) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGene TM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli ( https://www.genscript.com/gensmart-free-gene-codon-optimization.html ) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD 600 value reached 0.5–0.6, the culture medium was statically incubated at 15 °C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Clone Assay:Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer.
Article Snippet: In brief, we commercially synthesized a bsh (efpBSH) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGeneTM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli (https://www.genscript.com/gensmartfree-gene-codon-optimization.html) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD600 value reached 0.5–0.6, the culture medium was statically incubated at 15 ◦C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer
Article Snippet: In brief, we commercially synthesized a bsh ( efpBSH ) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGene TM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli ( https://www.genscript.com/gensmart-free-gene-codon-optimization.html ) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD 600 value reached 0.5–0.6, the culture medium was statically incubated at 15 °C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Plasmid Preparation:Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer.
Article Snippet: In brief, we commercially synthesized a bsh (efpBSH) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGeneTM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli (https://www.genscript.com/gensmartfree-gene-codon-optimization.html) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD600 value reached 0.5–0.6, the culture medium was statically incubated at 15 ◦C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer
Article Snippet: In brief, we commercially synthesized a bsh ( efpBSH ) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGene TM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli ( https://www.genscript.com/gensmart-free-gene-codon-optimization.html ) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD 600 value reached 0.5–0.6, the culture medium was statically incubated at 15 °C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Transformation Assay:Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer.
Article Snippet: In brief, we commercially synthesized a bsh (efpBSH) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGeneTM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli (https://www.genscript.com/gensmartfree-gene-codon-optimization.html) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD600 value reached 0.5–0.6, the culture medium was statically incubated at 15 ◦C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
Article Title: Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer
Article Snippet: In brief, we commercially synthesized a bsh ( efpBSH ) sequence (GenScript, Piscataway, NJ, USA). .. The OptimumGene TM algorithm software (GenScript) was used to optimize various parameters, including codon usage bias and GC content, which are critical for efficient heterologous gene expression in Escherichia coli ( https://www.genscript.com/gensmart-free-gene-codon-optimization.html ) (accessed on 6 January 2022). efpBSH was cloned into the NdeI and EcoRI sites of the pCold II vector (ampicillin resistant, TaKaRa, Tokyo, Japan) and transformed into E. coli BL21 (DE3) competent cells (SMOBIO Technology Inc., Hsinchu, Taiwan). .. When the OD 600 value reached 0.5–0.6, the culture medium was statically incubated at 15 °C for 30 min, and then isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque Inc., Kyoto, Japan) was added to the culture medium at the final concentration of 100 μM.
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