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columns  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec columns
    Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms/MS+Columns/pm42467720-253-19-20
    Average 97 stars, based on 2443 article reviews
    columns - by Bioz Stars, 2026-10
    97/100 stars

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    other:

    Article Title: Callitrichine herpesvirus 3 in the common marmoset is a model of Epstein-Barr virus infection and associated lymphoma.
    Article Snippet: 500 ng of DNase-treated RNA extracted from the CJ0149 cell line was reverse transcribed with random hexamer and SuperScript IV reverse transcriptase (Thermo Fisher Scientific).

    Mass Spectrometry:

    Article Title: Resistance to membrane damage enhances metastatic potential of breast cancer cells via dysregulated phospholipid profiles and annexin-mediated repair
    Article Snippet: .. Subsequently, the mixture was loaded onto a pre-equilibrated MS column (Miltenyi Biotec, 130-042-201) on a magnetic stand (Miltenyi Biotec, 130-041-109), washed once with 500 μL SuMA4 buffer, once with 500 μL SuMA2 buffer (SuMA supplemented with 0.5 mM DTT and 0.5% (w/v) fatty acid-free BSA and eluted in 600 μL SuMA2 buffer using the plunger after removal from the magnetic stand. ..

    Saline:

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Briefly, cortical tissue samples were obtained from adult mice following saline perfusion and cortical dissection, homogenized using a papain dissociation kit (Miltenyi Biotec, #130-107-677), incubated with Fc blocking antibody and magnetic anti-ACSA-2 microbeads (Miltenyi Biotec, #130-097-679), bound to MACS LS columns (Miltenyi Biotec, #130-042-201) placed on a magnetic stand, washed three times with 0.5% BSA buffer to remove unbound cells, and eluted for downstream application. .. RNA was extracted from isolated astrocytes by using the PicoPure RNA isolation kit (Applied Biosystems).

    Dissection:

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Briefly, cortical tissue samples were obtained from adult mice following saline perfusion and cortical dissection, homogenized using a papain dissociation kit (Miltenyi Biotec, #130-107-677), incubated with Fc blocking antibody and magnetic anti-ACSA-2 microbeads (Miltenyi Biotec, #130-097-679), bound to MACS LS columns (Miltenyi Biotec, #130-042-201) placed on a magnetic stand, washed three times with 0.5% BSA buffer to remove unbound cells, and eluted for downstream application. .. RNA was extracted from isolated astrocytes by using the PicoPure RNA isolation kit (Applied Biosystems).

    Incubation:

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Briefly, cortical tissue samples were obtained from adult mice following saline perfusion and cortical dissection, homogenized using a papain dissociation kit (Miltenyi Biotec, #130-107-677), incubated with Fc blocking antibody and magnetic anti-ACSA-2 microbeads (Miltenyi Biotec, #130-097-679), bound to MACS LS columns (Miltenyi Biotec, #130-042-201) placed on a magnetic stand, washed three times with 0.5% BSA buffer to remove unbound cells, and eluted for downstream application. .. RNA was extracted from isolated astrocytes by using the PicoPure RNA isolation kit (Applied Biosystems).

    Blocking Assay:

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Briefly, cortical tissue samples were obtained from adult mice following saline perfusion and cortical dissection, homogenized using a papain dissociation kit (Miltenyi Biotec, #130-107-677), incubated with Fc blocking antibody and magnetic anti-ACSA-2 microbeads (Miltenyi Biotec, #130-097-679), bound to MACS LS columns (Miltenyi Biotec, #130-042-201) placed on a magnetic stand, washed three times with 0.5% BSA buffer to remove unbound cells, and eluted for downstream application. .. RNA was extracted from isolated astrocytes by using the PicoPure RNA isolation kit (Applied Biosystems).

    Magnetic Cell Separation:

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Briefly, cortical tissue samples were obtained from adult mice following saline perfusion and cortical dissection, homogenized using a papain dissociation kit (Miltenyi Biotec, #130-107-677), incubated with Fc blocking antibody and magnetic anti-ACSA-2 microbeads (Miltenyi Biotec, #130-097-679), bound to MACS LS columns (Miltenyi Biotec, #130-042-201) placed on a magnetic stand, washed three times with 0.5% BSA buffer to remove unbound cells, and eluted for downstream application. .. RNA was extracted from isolated astrocytes by using the PicoPure RNA isolation kit (Applied Biosystems).



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    Image Search Results


    Reaction schemes and LC-MS analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla

    doi: 10.1016/j.synbio.2026.04.002

    Figure Lengend Snippet: Reaction schemes and LC-MS analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.

    Article Snippet: Liquid chromatography-mass spectrometry (LC-MS) was equipped with a YMC-Triart C18 column (250 mm × 4.6 mm, 5 μm), and the column temperature was set to 30 °C.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Transferring, In Vitro, Activity Assay, Recombinant, Control, Expressing, Plasmid Preparation, Tandem Mass Spectroscopy