ms Search Results


human anti fibroblast microbeads  (Miltenyi Biotec)
97
Miltenyi Biotec human anti fibroblast microbeads
Human Anti Fibroblast Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human anti fibroblast microbeads/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
human anti fibroblast microbeads - by Bioz Stars, 2026-05
97/100 stars
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bone tissue  (Invent Biotechnologies)
96
Invent Biotechnologies bone tissue
Bone Tissue, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bone tissue/product/Invent Biotechnologies
Average 96 stars, based on 1 article reviews
bone tissue - by Bioz Stars, 2026-05
96/100 stars
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human recombinant msp  (R&D Systems)
91
R&D Systems human recombinant msp
Human Recombinant Msp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
human recombinant msp - by Bioz Stars, 2026-05
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recombinant human msp cys672ala protein  (R&D Systems)
92
R&D Systems recombinant human msp cys672ala protein
Recombinant Human Msp Cys672ala Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human msp cys672ala protein/product/R&D Systems
Average 92 stars, based on 1 article reviews
recombinant human msp cys672ala protein - by Bioz Stars, 2026-05
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gpr171 inhibitor  (Tocris)
90
Tocris gpr171 inhibitor
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Gpr171 Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gpr171 inhibitor/product/Tocris
Average 90 stars, based on 1 article reviews
gpr171 inhibitor - by Bioz Stars, 2026-05
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laser beam  (Coherent Corp)
96
Coherent Corp laser beam
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Laser Beam, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/laser beam/product/Coherent Corp
Average 96 stars, based on 1 article reviews
laser beam - by Bioz Stars, 2026-05
96/100 stars
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ika ms  (IKA-Works inc)
94
IKA-Works inc ika ms
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Ika Ms, supplied by IKA-Works inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ika ms/product/IKA-Works inc
Average 94 stars, based on 1 article reviews
ika ms - by Bioz Stars, 2026-05
94/100 stars
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reagent nano kit v2  (Illumina Inc)
99
Illumina Inc reagent nano kit v2
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Reagent Nano Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reagent nano kit v2/product/Illumina Inc
Average 99 stars, based on 1 article reviews
reagent nano kit v2 - by Bioz Stars, 2026-05
99/100 stars
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illumina miseq sequencer  (Illumina Inc)
99
Illumina Inc illumina miseq sequencer
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Illumina Miseq Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illumina miseq sequencer/product/Illumina Inc
Average 99 stars, based on 1 article reviews
illumina miseq sequencer - by Bioz Stars, 2026-05
99/100 stars
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reagent kit v2  (Illumina Inc)
99
Illumina Inc reagent kit v2
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Reagent Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reagent kit v2/product/Illumina Inc
Average 99 stars, based on 1 article reviews
reagent kit v2 - by Bioz Stars, 2026-05
99/100 stars
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miseq micro kit v2  (Illumina Inc)
99
Illumina Inc miseq micro kit v2
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Miseq Micro Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miseq micro kit v2/product/Illumina Inc
Average 99 stars, based on 1 article reviews
miseq micro kit v2 - by Bioz Stars, 2026-05
99/100 stars
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standard sirolimus d3  (Toronto Research Chemicals)
91
Toronto Research Chemicals standard sirolimus d3
(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing <t>GPR171</t> surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.
Standard Sirolimus D3, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard sirolimus d3/product/Toronto Research Chemicals
Average 91 stars, based on 1 article reviews
standard sirolimus d3 - by Bioz Stars, 2026-05
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Image Search Results


(A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing GPR171 surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.

Journal: Science translational medicine

Article Title: Transcriptional networks in at-risk individuals identify signatures of type 1 diabetes progression

doi: 10.1126/scitranslmed.abd5666

Figure Lengend Snippet: (A and B) Radar plots showing age-corrected associations (Imm FDRT1D:FDRcontrol) of all module eigengenes (shown around plot circumference) with time to T1D onset in IAAs-first (A) and GADA-first (B) cases. Disease specificity indicated by a T1D:control significance ratio > 1 (−log10FDR) = red line. (C and D) Line plots showing individual and summary (inset) effects of natural cubic spline–fitted lmm (±95% CI) fitted to IAAsig (C) or GADAsig (D) eigenvalues in cases (left) and matched controls (right). (E and F) Radar plot (E) and barplot (F) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (E) and DICE (F) relational databanks. (G) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of IAAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. (H to J) Radar plot (H) and barplot (I) showing IAAs module enrichment for cell type–specific transcripts compared to ARCHS4 (H) and DICE (I) relational databanks. (J) Volcano plot (left) and line plots (right) showing all (left) and selected (right) correlations (Pearson) of GADAsig eigengenes against deconvoluted cell proportions. Significance threshold (FDR < 5%) = red dashed line. For barplots (F and I), TPM, transcripts per million reads; expression (means ± SEM) of IAAsig (F) and GADAsig (I) per cell type is shown. (K) Radar plot showing enrichment (−log10FDR) of IAAsig genes against 352 druggable targets linked to 20,883 genes from the IDG repository. Drug targets showing any overlap with IAAsig genes are included around the radar plot circumference. (L) Barplot (means ± SEM, n = 3 to 12 per group) showing IAAsig eigengene expression and (M) representative histograms showing GPR171 surface protein expression in circulating immune cell subsets (GSE22886). (N) Representative contour plot (top) and scatterplot (bottom) showing GPR171 surface protein expression (y axis, log10 MFI) and Granzyme B (GZMB) protein expression (x axis) after coculture of purified primary human NK cells with K562 target cells along with vehicle (left) or a titrated dose range of specific GPR171 inhibitor (Inh; right). *P < 0.05, MFI, median fluorescence index; Treg, regulatory T cell.

Article Snippet: Purified NK cells were cultured for 48 hours in complete RPMI 1640 in the presence of target K562 cells and either a GPR171 inhibitor (MS21570, Tocris Bio-Techne) or vehicle (phosphate-buffered saline) and stained with an excess of antibodies against GPR171 (polyclonal rabbit anti-human GPR171, Abcam), CD71 (clone CY1G4, BioLegend), and Granzyme B (clone GB11, BioLegend).

Techniques: Expressing, Purification, Fluorescence