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mpfc pyramidal neurons (Nikon)

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Nikon mpfc pyramidal neurons
Mpfc Pyramidal Neurons, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 63936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpfc/Objectives/pm41672997-142-1-12
Average 99 stars, based on 63936 article reviews

mpfc pyramidal neurons - by Bioz Stars, 2026-11

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Live Cell Imaging:

Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

Microscopy:

Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

Fluorescence:

Electron Microscopy:

Immunohistochemical staining:

Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

Staining:

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a. Schematic coronal brain section indicating the mPFC prelimbic region in dark grey, where tdTomato + parvalbumin (PV) cells and pyramidal (PYR) cells were recorded in APP/PS1 PV-Cre tdTomato (APP/PS1) and PV-Cre tdTomato (control) mice. Representative differential interference contrast images (left) and a corresponding fluorescent image (right). fmi= forceps minor of the corpus callosum. ML= midline. Recordings were performed in 16 ( b-i ) and 20 ( j-q ) week-old mice. b . Resting membrane potential was unaltered in PV cells at 16 weeks of age. Mann-Whitney test: U = 433, p = 0.98, n = 29/30 cells, N = 4 mice/genotype. Ctr= control. c . Action potential (AP) firing of PV cells upon a depolarizing current step (250 pA). d. AP frequency in PV cells in response to 0-425 pA depolarizing current steps did not differ between genotypes. Two-way repeated measures ANOVA genotype x current F (10,390) = 0.44, p = 0.93, n = 29/30 cells, N = 4 mice/genotype. e. Rheobase was unchanged in PV cells. Unpaired t-test: t 57 = 0.08, p = 0.93, n = 29/30 cells, N = 4 mice/genotype. Ctr= control. f . Resting membrane potential of PYR cells did not differ between genotypes. Unpaired t-test: t 36 = 0.51, p = 0.61, n = 17/21 cells from N = 4/6 control vs. APP/PS1 mice, respectively. Ctr= control. g. AP firing of PYR cells upon a depolarizing current step (250 pA). h. AP frequency in PYR cells in response to 0-250 pA depolarizing current steps did not differ between genotypes. Two-way repeated measures ANOVA genotype x current F (10,370) = 0.25, p = 0.99, n = 17/21 from N = 4/6 control vs. APP/PS1 mice, respectively. i. APP/PS1 mice show a lower rheobase in PYR cells. Mann-Whitney test: U = 111, * p = 0.048, n = 17/21 cells from N = 4/6 control vs. APP/PS1 mice, respectively. Ctr= control. j . Resting membrane potential was unaltered in PV cells at 20 weeks of age. Unpaired t-test: t 35 = 0.93, p = 0.36, n = 22/15 cells from N = 6/7 control and APP/PS1 mice, respectively. Ctr= control. k. AP firing of PV cells upon a depolarizing current step (250 pA). l. APP/PS1 mice show an increased AP frequency in PV cells in response to 0-425 pA depolarizing current steps. Two-way repeated measures ANOVA genotype x current F (17,595) = 4.05, * p < 0.0001, n = 22/15 cells, N = 6/7 control and APP/PS1 mice, respectively. m . Rheobase was unchanged in PV cells. Unpaired t-test: t 35 = 1.67, p = 0.10, 22/15 cells, N = 6/7 control and APP/PS1 mice, respectively. Ctr= control. n. Resting membrane potential of PYR cells did not differ between genotypes. Unpaired t-test: t 74 = 0.92, p = 0.36, n = 37/39 cells, N = 9 mice/genotype. Ctr= control. o . AP firing of PYR cells upon a depolarizing current step (250 pA). p . AP frequency in PYR cells in response to 0-250 pA depolarizing current steps did not differ between genotypes. Two-way repeated measures ANOVA genotype x current F (10,740) = 1.80, p = 0.08, n = 37/39 cells, N = 9 mice/genotype. q. APP/PS1 mice show a decrease in PYR cell rheobase. Unpaired t-test: t 74 = 2.34, * p = 0.022, n = 37/39 cells, N = 9 mice/genotype. Ctr= control. Graphs show mean ± s.e.m.

Journal: bioRxiv

Article Title: Progressive remote memory decline coincides with parvalbumin interneuron hyperexcitability and enhanced inhibition of cortical engram cells in a mouse model of Alzheimer’s disease

doi: 10.1101/2025.03.26.645537

Figure Lengend Snippet: a. Schematic coronal brain section indicating the mPFC prelimbic region in dark grey, where tdTomato + parvalbumin (PV) cells and pyramidal (PYR) cells were recorded in APP/PS1 PV-Cre tdTomato (APP/PS1) and PV-Cre tdTomato (control) mice. Representative differential interference contrast images (left) and a corresponding fluorescent image (right). fmi= forceps minor of the corpus callosum. ML= midline. Recordings were performed in 16 ( b-i ) and 20 ( j-q ) week-old mice. b . Resting membrane potential was unaltered in PV cells at 16 weeks of age. Mann-Whitney test: U = 433, p = 0.98, n = 29/30 cells, N = 4 mice/genotype. Ctr= control. c . Action potential (AP) firing of PV cells upon a depolarizing current step (250 pA). d. AP frequency in PV cells in response to 0-425 pA depolarizing current steps did not differ between genotypes. Two-way repeated measures ANOVA genotype x current F (10,390) = 0.44, p = 0.93, n = 29/30 cells, N = 4 mice/genotype. e. Rheobase was unchanged in PV cells. Unpaired t-test: t 57 = 0.08, p = 0.93, n = 29/30 cells, N = 4 mice/genotype. Ctr= control. f . Resting membrane potential of PYR cells did not differ between genotypes. Unpaired t-test: t 36 = 0.51, p = 0.61, n = 17/21 cells from N = 4/6 control vs. APP/PS1 mice, respectively. Ctr= control. g. AP firing of PYR cells upon a depolarizing current step (250 pA). h. AP frequency in PYR cells in response to 0-250 pA depolarizing current steps did not differ between genotypes. Two-way repeated measures ANOVA genotype x current F (10,370) = 0.25, p = 0.99, n = 17/21 from N = 4/6 control vs. APP/PS1 mice, respectively. i. APP/PS1 mice show a lower rheobase in PYR cells. Mann-Whitney test: U = 111, * p = 0.048, n = 17/21 cells from N = 4/6 control vs. APP/PS1 mice, respectively. Ctr= control. j . Resting membrane potential was unaltered in PV cells at 20 weeks of age. Unpaired t-test: t 35 = 0.93, p = 0.36, n = 22/15 cells from N = 6/7 control and APP/PS1 mice, respectively. Ctr= control. k. AP firing of PV cells upon a depolarizing current step (250 pA). l. APP/PS1 mice show an increased AP frequency in PV cells in response to 0-425 pA depolarizing current steps. Two-way repeated measures ANOVA genotype x current F (17,595) = 4.05, * p < 0.0001, n = 22/15 cells, N = 6/7 control and APP/PS1 mice, respectively. m . Rheobase was unchanged in PV cells. Unpaired t-test: t 35 = 1.67, p = 0.10, 22/15 cells, N = 6/7 control and APP/PS1 mice, respectively. Ctr= control. n. Resting membrane potential of PYR cells did not differ between genotypes. Unpaired t-test: t 74 = 0.92, p = 0.36, n = 37/39 cells, N = 9 mice/genotype. Ctr= control. o . AP firing of PYR cells upon a depolarizing current step (250 pA). p . AP frequency in PYR cells in response to 0-250 pA depolarizing current steps did not differ between genotypes. Two-way repeated measures ANOVA genotype x current F (10,740) = 1.80, p = 0.08, n = 37/39 cells, N = 9 mice/genotype. q. APP/PS1 mice show a decrease in PYR cell rheobase. Unpaired t-test: t 74 = 2.34, * p = 0.022, n = 37/39 cells, N = 9 mice/genotype. Ctr= control. Graphs show mean ± s.e.m.

Article Snippet: Pyramidal, PV and SST cells were recorded in the mPFC using a Multiclamp 700B amplifier (Molecular devices, Sunnyvale, CA) and sampled at 10 kHz low pass filter at 4 kHz and digitized with Axon Digidata 1440 A (Molecular Devices).

Techniques: Control, Membrane, MANN-WHITNEY

SST cell excitability is unaltered in the mPFC of 20-week-old APP/PS1 mice. a. Schematic coronal brain section indicating the mPFC prelimbic region in dark grey, where AAV-hSyn::DIO-mCherry was microinjected and mCherry + SST cells were recorded in APP/PS1 SST-Cre (APP/PS1) and SST-Cre (control) mice. Representative fluorescent image is depicted b. Resting membrane potential was unaltered in SST cells. Unpaired t-test: t 31 = 0.73, p = 0.47, n = 17/16 cells, N = 5/7 control vs. APP/PS1 mice, respectively. c. Action potential (AP) firing of SST cells upon a depolarizing current step (250 pA) d. AP frequency in SST cells in response to 0-250 pA depolarizing current steps did not differ between genotypes. Genotype x current two-way repeated measures ANOVA F (10,310) = 0.23, p = 0.99, n = 17/16 cells, N = 5/7 control vs. APP/PS1 mice. e. Rheobase was unchanged in SST cells Mann-Whitney test: U = 119, p = 0.75, n = 17/16 cells, N = 5/7 control vs. APP/PS1 mice, respectively. Graphs show mean ± s.e.m.

Journal: bioRxiv

doi: 10.1101/2025.03.26.645537

Figure Lengend Snippet: SST cell excitability is unaltered in the mPFC of 20-week-old APP/PS1 mice. a. Schematic coronal brain section indicating the mPFC prelimbic region in dark grey, where AAV-hSyn::DIO-mCherry was microinjected and mCherry + SST cells were recorded in APP/PS1 SST-Cre (APP/PS1) and SST-Cre (control) mice. Representative fluorescent image is depicted b. Resting membrane potential was unaltered in SST cells. Unpaired t-test: t 31 = 0.73, p = 0.47, n = 17/16 cells, N = 5/7 control vs. APP/PS1 mice, respectively. c. Action potential (AP) firing of SST cells upon a depolarizing current step (250 pA) d. AP frequency in SST cells in response to 0-250 pA depolarizing current steps did not differ between genotypes. Genotype x current two-way repeated measures ANOVA F (10,310) = 0.23, p = 0.99, n = 17/16 cells, N = 5/7 control vs. APP/PS1 mice. e. Rheobase was unchanged in SST cells Mann-Whitney test: U = 119, p = 0.75, n = 17/16 cells, N = 5/7 control vs. APP/PS1 mice, respectively. Graphs show mean ± s.e.m.

Techniques: Control, Membrane, MANN-WHITNEY

Journal: bioRxiv

doi: 10.1101/2025.03.26.645537

Figure Lengend Snippet:

Techniques: Membrane

a . Coronal brain section indicating the mPFC region (dark grey) where AAV-Fos::CreER T2 and Cre-dependent AAV-hSyn::DIO-mCherry were injected. Mice underwent CFC at 16 weeks old and engram cells were tagged. Thirty days after CFC, mice were re-exposed to the training context and then immediately sacrificed for whole-cell patch-clamp electrophysiology. b . Left: Representative image showing labeled mCherry + engram cells in the mPFC. Right: recordings were made from mCherry + and mCherry - pyramidal cells. c . Example sIPSC traces of mCherry + and mCherry - for WT control and APP/PS1 mice d . Example sEPSC traces of mCherry + and mCherry - cells for WT and APP/PS1 mice e. Frequency of sIPSCs differed between mCherry + and mCherry - cells in APP/PS1, but not WT, mice. Two-way repeated measure ANOVA genotype x cell-type F (1,43) = 5.44, * p = 0.024. Post-hoc Bonferroni APP/PS1 mCherry + vs. mCherry - * p = 0.011. n = 22 per cell-type from N = 6 WT mice, n = 23 per cell-type from N = 7 APP/PS1 mice. f. sIPSC amplitude did not differ between cell-type and genotype. g. Frequency of sEPSCs was enhanced in mCherry + cells compared to mCherry - cells in both genotypes. Two-way repeated measure ANOVA cell-type F (1,36) = 7.26, * p = 0.011 n = 20 per cell type from N = 6 WT mice, n = 23 per cell-type from N = 7 APP/PS1 mice h. sEPSC amplitude did not differ between cell-type and genotype. Graphs show mean ± s.e.m.

Journal: bioRxiv

doi: 10.1101/2025.03.26.645537

Figure Lengend Snippet: a . Coronal brain section indicating the mPFC region (dark grey) where AAV-Fos::CreER T2 and Cre-dependent AAV-hSyn::DIO-mCherry were injected. Mice underwent CFC at 16 weeks old and engram cells were tagged. Thirty days after CFC, mice were re-exposed to the training context and then immediately sacrificed for whole-cell patch-clamp electrophysiology. b . Left: Representative image showing labeled mCherry + engram cells in the mPFC. Right: recordings were made from mCherry + and mCherry - pyramidal cells. c . Example sIPSC traces of mCherry + and mCherry - for WT control and APP/PS1 mice d . Example sEPSC traces of mCherry + and mCherry - cells for WT and APP/PS1 mice e. Frequency of sIPSCs differed between mCherry + and mCherry - cells in APP/PS1, but not WT, mice. Two-way repeated measure ANOVA genotype x cell-type F (1,43) = 5.44, * p = 0.024. Post-hoc Bonferroni APP/PS1 mCherry + vs. mCherry - * p = 0.011. n = 22 per cell-type from N = 6 WT mice, n = 23 per cell-type from N = 7 APP/PS1 mice. f. sIPSC amplitude did not differ between cell-type and genotype. g. Frequency of sEPSCs was enhanced in mCherry + cells compared to mCherry - cells in both genotypes. Two-way repeated measure ANOVA cell-type F (1,36) = 7.26, * p = 0.011 n = 20 per cell type from N = 6 WT mice, n = 23 per cell-type from N = 7 APP/PS1 mice h. sEPSC amplitude did not differ between cell-type and genotype. Graphs show mean ± s.e.m.

Techniques: Injection, Patch Clamp, Labeling, Control

A . UMAP plot showing clustering of Tac2 neurons with other defined cortical cell types. B . Bar graph showing the proportion of excitatory and inhibitory neurons that express Tac2 . C . Bar graph showing the proportion of Tac2 counts in cortical cell types. D-G . Representative images of RNAscope™ fluorescent in situ hybridization. scale bar represents 100μm. Tac2: Tachykinin-2, CRF: corticotropin releasing factor, SOM: somatostatin, PV: parvalbumin, VIP: Vasoactive intestinal peptide. H . Diagram showing prelimbic and infralimbic regions of medial prefrontal cortex with cortical layers. L1-3: layers 1-3, L5: layer 5, L6: layer 6. I . Pie chart showing percent of Tac2 cells expressing CRF (N=6 animals, 3 GH, 3 SI; 2 Male, ∼ 3 sections/animal), SOM (N=4 animals, 2 GH, 2 SI; 1 Male, ∼3 sections/animal), PV (N=6 animals, 3 GH, 3 SI; 4 Male, ∼3 sections/animal), and VIP (N=8 animals (4 GH, 4 SI; 4M). J . Pie chart showing the percent of Tac2 cells in cortical layers. K . Pie chart showing the percent of Tac2 cells that percent VIP in early (L1-3) and deep (L5-6) cortical layers. L . Diagram showing prelimbic and infralimbic regions of medial prefrontal cortex with cortical layers. L1-3: layers 1-3, L5: layer 5, L6: layer 6. M . Representative image showing Tac2 cFos in situ hybridization across the cortical layers. Bar represents 100µm. N . Bar graph showing the percent of Tac2 cells expressing cFos in layers 1-3 (N = 4, 2 SI, 2 GH, 2 Males, ∼ 3 sections/animal); nested t-test p < 0.01. O . Bar graph showing the percent of Tac2 cells expressing cFos in layer 5 (N = 4, 2 SI, 2 GH, 2 Males, ∼3 sections/animal); nested t-test p > 0.05. P . Bar graph showing the percent of Tac2 cells expressing cFos in layer 6 (N = 4, 2 SI, 2 GH, 2 Males, ∼3 sections/animal); nested t-test p > 0.05. Q . Diagram showing viral infusion into infralimbic mPFC. R . Representative image showing Tac2 neuron axons labeled with GFP and the cortical layers. S . Representative image showing Tac2 neuronal terminals (synaptophysin) in red (mRuby). T . Merged image showing axonal arbor (green) and terminals (red) of Tac2 neurons. R-T scale bar represents 100μm. Bars are mean ± SEM ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: bioRxiv

Article Title: Co-release of opposing signaling molecules from cortical neurons controls the escalation and release of aggression

doi: 10.1101/2025.03.13.643119

Figure Lengend Snippet: A . UMAP plot showing clustering of Tac2 neurons with other defined cortical cell types. B . Bar graph showing the proportion of excitatory and inhibitory neurons that express Tac2 . C . Bar graph showing the proportion of Tac2 counts in cortical cell types. D-G . Representative images of RNAscope™ fluorescent in situ hybridization. scale bar represents 100μm. Tac2: Tachykinin-2, CRF: corticotropin releasing factor, SOM: somatostatin, PV: parvalbumin, VIP: Vasoactive intestinal peptide. H . Diagram showing prelimbic and infralimbic regions of medial prefrontal cortex with cortical layers. L1-3: layers 1-3, L5: layer 5, L6: layer 6. I . Pie chart showing percent of Tac2 cells expressing CRF (N=6 animals, 3 GH, 3 SI; 2 Male, ∼ 3 sections/animal), SOM (N=4 animals, 2 GH, 2 SI; 1 Male, ∼3 sections/animal), PV (N=6 animals, 3 GH, 3 SI; 4 Male, ∼3 sections/animal), and VIP (N=8 animals (4 GH, 4 SI; 4M). J . Pie chart showing the percent of Tac2 cells in cortical layers. K . Pie chart showing the percent of Tac2 cells that percent VIP in early (L1-3) and deep (L5-6) cortical layers. L . Diagram showing prelimbic and infralimbic regions of medial prefrontal cortex with cortical layers. L1-3: layers 1-3, L5: layer 5, L6: layer 6. M . Representative image showing Tac2 cFos in situ hybridization across the cortical layers. Bar represents 100µm. N . Bar graph showing the percent of Tac2 cells expressing cFos in layers 1-3 (N = 4, 2 SI, 2 GH, 2 Males, ∼ 3 sections/animal); nested t-test p < 0.01. O . Bar graph showing the percent of Tac2 cells expressing cFos in layer 5 (N = 4, 2 SI, 2 GH, 2 Males, ∼3 sections/animal); nested t-test p > 0.05. P . Bar graph showing the percent of Tac2 cells expressing cFos in layer 6 (N = 4, 2 SI, 2 GH, 2 Males, ∼3 sections/animal); nested t-test p > 0.05. Q . Diagram showing viral infusion into infralimbic mPFC. R . Representative image showing Tac2 neuron axons labeled with GFP and the cortical layers. S . Representative image showing Tac2 neuronal terminals (synaptophysin) in red (mRuby). T . Merged image showing axonal arbor (green) and terminals (red) of Tac2 neurons. R-T scale bar represents 100μm. Bars are mean ± SEM ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Male and female Tac2-IRES-Cre mice 8-10 weeks were single housed and had surgeries performed to infuse a Cre-dependent adeno-associated virus (AAV) expressing membrane-GFP and synaptophysin fused to mRuby (AAV2-hSynb-FLEx-mGFP-2A-Synaptophysin-mRuby) infused into the mPFC (Addgene #: 71760-AAV2, titer: 2.7 x 10 GC/mL, lot: v138755).

Techniques: RNAscope, In Situ Hybridization, Expressing, Labeling

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mpfc pyramidal neurons (Nikon)

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