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recombinant mouse leptin  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse leptin
    Recombinant Mouse Leptin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+leptin/Recombinant+Mouse+Leptin+Protein%2C+CF/pm42017526-76-0-3
    Average 95 stars, based on 210 article reviews
    recombinant mouse leptin - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Leptin Reduces Running in a Rodent Anorexia Nervosa Model via a Distributed Neural Network.
    Article Snippet: .. Recombinant mouse leptin (R&D Systems, USA) was stored at −20°C and freshly prepared before use by dissolving in phosphate‐buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3) at 1 μg/μL concentration. ..

    Article Title: TonEBP as a key regulator of hypothalamic leptin signaling and resistance.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Following a one-week recovery period in individual cages, mice fasted for 24 h were administered recombinant mouse leptin (1 or 3 μg/2 μl/mouse; R&D Systems, Minneapolis, MN) or saline (vehicle control) via Intracerebroventricular (i.c.v.) injection. ..

    Article Title: A uniquely leptin sensitive hypothalamic neuron population limits hyperphagia and weight gain in diet-induced obesity
    Article Snippet: .. Mouse recombinant leptin (R&D Systems, #498-OB) was reconstituted to 1 mg/ml in 20 mM Tris-HCl pH 8.0 (Invitrogen, #10434742). ..

    Article Title: Phenethyl isothiocyanate ameliorates high-fat diet-induced obesity by antagonizing hypothalamic leptin resistance
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Saline:

    Article Title: Leptin Reduces Running in a Rodent Anorexia Nervosa Model via a Distributed Neural Network.
    Article Snippet: .. Recombinant mouse leptin (R&D Systems, USA) was stored at −20°C and freshly prepared before use by dissolving in phosphate‐buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3) at 1 μg/μL concentration. ..

    Article Title: TonEBP as a key regulator of hypothalamic leptin signaling and resistance.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Following a one-week recovery period in individual cages, mice fasted for 24 h were administered recombinant mouse leptin (1 or 3 μg/2 μl/mouse; R&D Systems, Minneapolis, MN) or saline (vehicle control) via Intracerebroventricular (i.c.v.) injection. ..

    Article Title: Metabolic regulation by tanycyte-derived extracellular vesicles through insulin precursor-mediated neuronal recognition and mTORC component delivery.
    Article Snippet: The hypothalamus regulates feeding and metabolic balance in response to metabolic cues.. Here we report that extracellular vesicles (EVs) are secreted from the mediobasal hypothalamus in a diurnal manner that is influenced by daily feeding.. Sox2-positive tanycytes have a critical role in maintaining the diurnal pattern of hypothalamic EV release.

    Concentration Assay:

    Article Title: Leptin Reduces Running in a Rodent Anorexia Nervosa Model via a Distributed Neural Network.
    Article Snippet: .. Recombinant mouse leptin (R&D Systems, USA) was stored at −20°C and freshly prepared before use by dissolving in phosphate‐buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3) at 1 μg/μL concentration. ..

    Control:

    Article Title: TonEBP as a key regulator of hypothalamic leptin signaling and resistance.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Following a one-week recovery period in individual cages, mice fasted for 24 h were administered recombinant mouse leptin (1 or 3 μg/2 μl/mouse; R&D Systems, Minneapolis, MN) or saline (vehicle control) via Intracerebroventricular (i.c.v.) injection. ..

    Article Title: Metabolic regulation by tanycyte-derived extracellular vesicles through insulin precursor-mediated neuronal recognition and mTORC component delivery.
    Article Snippet: The hypothalamus regulates feeding and metabolic balance in response to metabolic cues.. Here we report that extracellular vesicles (EVs) are secreted from the mediobasal hypothalamus in a diurnal manner that is influenced by daily feeding.. Sox2-positive tanycytes have a critical role in maintaining the diurnal pattern of hypothalamic EV release.

    Injection:

    Article Title: TonEBP as a key regulator of hypothalamic leptin signaling and resistance.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Following a one-week recovery period in individual cages, mice fasted for 24 h were administered recombinant mouse leptin (1 or 3 μg/2 μl/mouse; R&D Systems, Minneapolis, MN) or saline (vehicle control) via Intracerebroventricular (i.c.v.) injection. ..

    Article Title: Metabolic regulation by tanycyte-derived extracellular vesicles through insulin precursor-mediated neuronal recognition and mTORC component delivery.
    Article Snippet: The hypothalamus regulates feeding and metabolic balance in response to metabolic cues.. Here we report that extracellular vesicles (EVs) are secreted from the mediobasal hypothalamus in a diurnal manner that is influenced by daily feeding.. Sox2-positive tanycytes have a critical role in maintaining the diurnal pattern of hypothalamic EV release.

    Sterility:

    Article Title: A catecholamine-independent pathway controlling adaptive adipocyte lipolysis.
    Article Snippet: .. In brief, osmotic pumps (Alzet, model 1002) were filled according to the manufacturer’s instructions with sterile PBS or leptin (R&D Systems, 498-OB) reconstituted with PBS. .. For ICV surgeries, brain infusion cannulas and catheter tubes (Alzet, Brain Infusion Kit 3) were also filled and connected to the pumps.

    Article Title: A catecholamine-independent pathway controlling adaptive adipocyte lipolysis
    Article Snippet: .. In brief, osmotic pumps (Alzet, model 1002) were filled according to the manufacturer’s instructions with sterile PBS or leptin (R&D Systems, 498-OB) reconstituted with PBS. .. For ICV surgeries, brain infusion cannulas and catheter tubes (Alzet, Brain Infusion Kit 3) were also filled and connected to the pumps.



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    Multi Sciences (Lianke) Biotech Co Ltd leptin
    Insulin resistance and metabolic profile of mice treated with different carbohydrate-fat ratio diets. (A) Fasting serum insulin levels(FINS) in mice (n = 6). (B) Homeostasis Model Assessment-Insulin resistance (HOMA-IR) in mice (n = 6). (C) Triglyceride glucose (TyG) index in mice (n = 6). (D–G) Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density cholesterol (HDL-C) levels (n = 6). (H) <t>Serum</t> <t>adiponectin</t> levels in mice (n = 6). (I) Serum <t>leptin</t> levels in mice (n = 6). (J) Serum TNF-α levels in mice (n = 6). (K) Serum IL-1β levels in mice (n = 6). (L) Serum IL-6 levels in mice (n = 6). In statistical analysis, all values are expressed as mean ± SD. In statistical analysis, all values are expressed as mean ± SD. * indicates statistical difference between the model and control groups, * P < 0.05, ** P < 0.01, *** P < 0.001.
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    Image Search Results


    Insulin resistance and metabolic profile of mice treated with different carbohydrate-fat ratio diets. (A) Fasting serum insulin levels(FINS) in mice (n = 6). (B) Homeostasis Model Assessment-Insulin resistance (HOMA-IR) in mice (n = 6). (C) Triglyceride glucose (TyG) index in mice (n = 6). (D–G) Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density cholesterol (HDL-C) levels (n = 6). (H) Serum adiponectin levels in mice (n = 6). (I) Serum leptin levels in mice (n = 6). (J) Serum TNF-α levels in mice (n = 6). (K) Serum IL-1β levels in mice (n = 6). (L) Serum IL-6 levels in mice (n = 6). In statistical analysis, all values are expressed as mean ± SD. In statistical analysis, all values are expressed as mean ± SD. * indicates statistical difference between the model and control groups, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Endocrinology

    Article Title: Effects of different sugar-lipid ratio diets on the occurrence of type 2 diabetes mellitus

    doi: 10.3389/fendo.2026.1768263

    Figure Lengend Snippet: Insulin resistance and metabolic profile of mice treated with different carbohydrate-fat ratio diets. (A) Fasting serum insulin levels(FINS) in mice (n = 6). (B) Homeostasis Model Assessment-Insulin resistance (HOMA-IR) in mice (n = 6). (C) Triglyceride glucose (TyG) index in mice (n = 6). (D–G) Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density cholesterol (HDL-C) levels (n = 6). (H) Serum adiponectin levels in mice (n = 6). (I) Serum leptin levels in mice (n = 6). (J) Serum TNF-α levels in mice (n = 6). (K) Serum IL-1β levels in mice (n = 6). (L) Serum IL-6 levels in mice (n = 6). In statistical analysis, all values are expressed as mean ± SD. In statistical analysis, all values are expressed as mean ± SD. * indicates statistical difference between the model and control groups, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Additionally, serum levels of adiponectin (ED295; Lianke Bio, China) and leptin (EK-297; Lianke Bio, China) were analyzed using their respective ELISA kits.

    Techniques: Control