african green monkey kidney fibroblast  (ATCC)

Journal: Molecular Therapy Advances

Article Title: Efficacy and safety of SENS-501, a dual-AAV otoferlin gene therapy, for DFNB9 congenital deafness

doi: 10.1016/j.omta.2026.201762

Figure Lengend Snippet: Intracochlear injection of SENS-501 in NHPs results in a mild humoral and an undetectable cellular response to the capsid (A) AAV8 anti-drug antibody (ADA) titers measured in serum at pre-dose and at 16, 29, and 92 days post-vector administration in NHPs. (B) Anti-AAV8 neutralizing antibodies (NAb) titers measured in serum at pre-dose and at 16, 29, and 92 days post-vector administration in NHPs. (C and D) IFN-γ spot forming units (SFUs) measured by ELISpot assay at 29 (C; left) and 92 (D; right) days post-injection. Peripheral blood mononuclear cells (PBMCs) from the indicated groups were stimulated with three different AAV8 peptide pools and a positive control (PMA/ionomycin). The dotted line represents the assay-specific positivity threshold. Each dot represents one animal. Bars represent the mean ± SEM.

Article Snippet: After the incubation, detection was performed with a monoclonal anti-monkey IFN-γ antibody (Monkey IFN-γ ELISpot Pro Kit, Mabtech) coupled with alkaline phosphatase and incubated with BCIP/NBT (5-bromo-4-chloro-3-indolyl-1-phosphate / nitroblue tetrazolium) substrate to detect secreted IFN-γ.

Techniques: Injection, Plasmid Preparation, Enzyme-linked Immunospot, Positive Control

Journal: iScience

Article Title: RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation

doi: 10.1016/j.isci.2026.116284

Figure Lengend Snippet: Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. (C–J) HEK293T cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) Vero cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).

Article Snippet: Human embryonic kidney (HEK293T), human lung adenocarcinoma (A549), and African green monkey kidney (Vero) cells were purchased from the American Type Culture Collection (ATCC), IFNAR-knockout A549 cells were kindly provided by Prof. Genhong Cheng (Guangzhou National Laboratory).

Techniques: Sequencing, Control, Infection, Plaque Assay, Transfection, Western Blot, Plasmid Preparation, Immunofluorescence, Staining, Microscopy