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nebnext poly a mrna magnetic isolation module kit  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext poly a mrna magnetic isolation module kit
    Nebnext Poly A Mrna Magnetic Isolation Module Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/module/NEBNext+Poly(A)+mRNA+Magnetic+Isolation+Module/pmc13634473-258-6-6
    Average 99 stars, based on 6816 article reviews
    nebnext poly a mrna magnetic isolation module kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Molecular Phylogeny and Taxonomic Revision of the Dictyostelid Social Amoebae (Dictyostelia, Eumycetozoa).
    Article Snippet: Total RNA was extracted from 108 cells with the Qiagen RNeasy Midi Kit (#75144). .. Libraries were prepared using the NEBNext Ultra2 Directional RNA library kit (#E7760S) with the NEBNext Poly(A)mRNA Magnetic Isolation Module (#E7490S). ..

    Article Title: Integrated Single‐Myofibre Multi‐Omics Correlates Inflammatory State to Myosin Energetics in ICU‐Acquired Weakness
    Article Snippet: .. mRNA was captured from 10 μL of fibre lysate using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions. ..

    Article Title: Disentangling microbiota-dependent and -independent impacts of antibiotics on host physiology.
    Article Snippet: The integrity of the isolated RNA was analysed using an Agilent High Sensitivity RNA ScreenTape Kit and TapeStation 4200 at the Genomic Technology Center of the Azrieli Faculty of Medicine at Bar-Ilan University. .. 1,000 ng total RNA was treated with a NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490L; New England Biolabs). .. RNA-seq libraries were produced using the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770L; New England Biolabs).

    Article Title: The Maternal Blood Transcriptome Reflects Molecular Signatures Associated With Large Offspring Syndrome and Other Adverse Pregnancy Outcomes in Cattle
    Article Snippet: Sequencing was performed at the Interdisciplinary Center of Biotechnology Research NextGen Sequencing Core ( https://biotech.ufl.edu/next‐gen‐dna/ , RRID:SCR_019152). .. First, 500 ng of total RNA was used for mRNA isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA, catalog #E7490). .. Then, RNA library construction was performed with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, catalog #E7760) with 2/3 of recommended poly A enriched RNA and 1/4 recommended reaction volume using SPT Labtech mosquito LV liquid handling instrument (Tecan, Männedorf, Switzerland).

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Article Title: The homologous GRAS transcription factors RAM1 and RAD1 coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis.
    Article Snippet: RNA-sequencing For all experiments, RNA samples were quality-checked using gel electrophoresis and bioanalyzer (Agilent, Santa Clara, CA, USA) .. For the AM experiment, mRNAs were enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA). .. Libraries were prepared using NEBNextUltra RNALibrary Prep Kit for Illumina (NEB) and sequenced on aNovaSeq 6000 S4 platform by BGI, Hong Kong (paired-end 150 bp).

    Article Title: Ecology drives the degree of convergence in the gene expression of extremophile fishes
    Article Snippet: .. We then isolated mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Inc., Ipswich, MA, USA) and constructed cDNA libraries with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA). .. All cDNA libraries were individually barcoded, quantified using Qubit (Thermo Fisher Scientific, MA, USA) and an Agilent 2100 Bioanalyzer High Sensitivity DNA chip (Agilent, CA, USA), and pooled in sets of 11–12 samples based on nanomolar concentrations.

    Selection:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (NEB #E7490, Ipswich, MA, USA) for Illumina with poly(A) selection and subjected to 151-bp paired-end sequencing on an Illumina NovaSeq platform (San Diego, CA, USA), generating an average of approximately 40 million paired reads per sample. ..

    Sequencing:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (NEB #E7490, Ipswich, MA, USA) for Illumina with poly(A) selection and subjected to 151-bp paired-end sequencing on an Illumina NovaSeq platform (San Diego, CA, USA), generating an average of approximately 40 million paired reads per sample. ..

    RNA Library Preparation:

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Multiplex Assay:

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Transcriptomics:

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Construct:

    Article Title: Ecology drives the degree of convergence in the gene expression of extremophile fishes
    Article Snippet: .. We then isolated mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Inc., Ipswich, MA, USA) and constructed cDNA libraries with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA). .. All cDNA libraries were individually barcoded, quantified using Qubit (Thermo Fisher Scientific, MA, USA) and an Agilent 2100 Bioanalyzer High Sensitivity DNA chip (Agilent, CA, USA), and pooled in sets of 11–12 samples based on nanomolar concentrations.



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