rpmi 1640 medium atcc modification (ATCC)

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ATCC rpmi 1640 medium atcc modification
Rpmi 1640 Medium Atcc Modification, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 19666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modifications/RPMI-1640+Medium/us12649798-951-22-25
Average 99 stars, based on 19666 article reviews

rpmi 1640 medium atcc modification - by Bioz Stars, 2026-11

99/100 stars

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Cell Culture:

Article Title: Metabolic reprogramming of macrophage-specific transketolase orchestrates angiogenesis in diabetic wounds
Article Snippet: .. Human umbilical vein endothelial cells (HUVECs) were obtained from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin at 37 °C in 5% CO2. ..

Article Title: Immunoregulatory gene GIMAP6 suppresses lethal atherosclerotic vasculopathy and ischemic heart failure.
Article Snippet: .. THP-1 cells (ATCC, TIB-202; RRID: CVCL_0006; human male, acute monocytic leukemia-derived monocytic cell line) were purchased from ATCC and cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 2 mM L-glutamine, and 1% penicillin-streptomycin. ..

Article Title: Hypoxia‐Driven CA2 Promotes Trophoblast Dysfunction in Preeclampsia
Article Snippet: The human trophoblastic cell line (HTR‐8/SVneo) was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. Cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI‐1640, cat. no 30‐2001, ATCC, USA), supplemented with 10% fetal bovine serum (FBS, Gibco, Australia) and 1% penicillin and streptomycin (VivaCell, Shanghai, China). ..

Article Title: Type I IFN promotes ILC2-to-ILC1 plasticity in pre-clinical radiation-induced lung injury
Article Snippet: Red blood cells were lysed with ACK lysis buffer (Invitrogen, Cat# 00-4333-57), and single-cell suspensions were filtered through a 70-μm cell strainer (Fisherbrand, Cat# 22363548). .. Cells were cultured in complete RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS, ATCC, Cat30-2020), 1% penicillin-streptomycin (ATCC, Cat# 30-2300), 20 ng/mL of IL-2 (PeproTech, Cat# 212-12-20UG), 20 ng/mL of IL-7 (PeproTech, Cat# 217-17-50UG), 20 ng/mL of IL-25 (PeproTech, Cat# 210-17E-25UG), and 20 ng/mL of IL-33 (PeproTech, Cat# 210-33-50UG) at a density of 1 × 105 cells/mL for 14 days. ..

Lysis:

Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026

Extraction:

Modification:

Recombinant:

Western Blot:

Transfection:

Membrane:

Tissue:

Article Title: Pharmacological targeting of FKBPL with rapamycin sensitizes triple-negative breast cancer to ferroptosis.
Article Snippet: .. Cell lines and tissue culture The human TNBC cell lines (BT549, MDA-MB-231), human cholangiocarcinoma cell line (RBE) and human lung cancer cell line (NCI-H460) were obtained from ATCC and maintained in RPMI 1640 medium (Procell system, China). .. The human embryonic kidney cell line (293T) and the non-tumorigenic mammary epithelial cell line (MCF-10A) were cultured in DMEM medium (Gibco, China).

Protease Inhibitor:

SYBR Green Assay:

Multiple Displacement Amplification:

other:

Article Title: In vivo Gene Writing with engineered retrotransposons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p21 Waf1/Cip1 (12D1) Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 2947S, RRID: AB_823586 TBP (D5C9H) Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 44059S, RRID: AB_2799258 Vinculin (E1E9V) Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 13901S, RRID: AB_2728768 Brilliant Violet 421 anti-human CD4 Biolegend Cat# 317434, RRID: AB_2562134 BD Horizon BV480 Mouse Anti-Human CD8 BD Biosciences Cat# 566121, RRID: AB_2739523 PE anti-human CD366 (Tim-3) Biolegend Cat# 345006, RRID: AB_2116576 Brilliant Violet 605 anti-human CD223 (LAG-3) Biolegend Cat# 369324, RRID: AB_2721541 PerCP anti-human CD279 (PD-1) Biolegend Cat# 329938, RRID: AB_2563596 APC/Cyanine7 anti-human CD45RA Biolegend Cat# 304128, RRID: AB_10708880 FITC anti-human CD62L Biolegend Cat# 304804, RRID: AB_314464 PE anti-human CD25 Biolegend Cat# 356104, RRID: AB_2561861 APC/Cyanine7 anti-human CD69 Biolegend Cat# 310914, RRID: AB_314849 G4S Linker (E7O2V) Rabbit Monoclonal Antibody (PE Conjugate) Cell Signaling Technology Cat# 38907L, RRID: AB_3626304 G4S Linker (E702V) Rabbit Monoclonal Antibody (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat# 69782L, RRID: AB_3101749 Brilliant Violet 605 anti-human CD3 Biolegend Cat# 317322, RRID: AB_2561911 APC/Cyanine7 anti-human CD14 Biolegend Cat# 301820, RRID: AB_493695 Brilliant Violet 421 anti-human CD3 Biolegend Cat# 317344, RRID: AB_2565849 Brilliant Violet 605 anti-human CD4 Biolegend Cat# 317438, RRID: AB_11218995 Brilliant Violet 650 anti-human CD197 (CCR7) Biolegend Cat# 353234, RRID: AB_2563867 Brilliant Violet 785 anti-human CD45RA Biolegend Cat# 304140, RRID: AB_2563816 PE anti-human CD8 Biolegend Cat# 344706, RRID: AB_1953244 PE/Cyanine5 anti-mouse CD45 Biolegend Cat# 103110, RRID: AB_312975 PE/Cyanine7 anti-human CD45 Biolegend Cat# 304016, RRID: AB_314404 PE/Dazzle 594 anti-human CD5 BD Biosciences Cat# 364012, RRID: AB_2565282 BD Horizon R718 Mouse Anti-Human CD19 Biolegend Cat# 567343, RRID: AB_2916561 FITC anti-human CD20 Biolegend Cat# 302304, RRID: AB_314252 PerCP/Cyanine5.5 anti-human CD159a (NKG2A) Biolegend Cat# 375126, RRID: AB_2922592 Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (APC Conjugate) Cell Signaling Technology Cat# 47314S Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat# 63910L, RRID: AB_3752579 Chemicals, peptides, and recombinant proteins TexMACS medium Miltenyi Cat# 130-097-196 BenchStable DMEM Gibco Cat# A4192101 BenchStable RPMI 1640 Gibco Cat# A4192301 RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 DMEM/F12 Thermo Fisher Scientific Cat# 10565018 mTeSR-Plus media STEMCELL Technologies Cat# 100-0276 Gentle Cell Dissociation Reagent STEMCELL Technologies Cat# 100-0485 ReLeSR reagent STEMCELL Technologies Cat# 100-0483 P3 Primary Cell Nucleofection Buffer Lonza Cat# V4SP-3096 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–19.e1–e14, December 24, 2026 Article

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