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mneongreen coding sequences  (Addgene inc)


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    Addgene inc mneongreen coding sequences
    Mneongreen Coding Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mneongreen+coding+sequence/mneongreen+coding+sequence/pm38778742-376-3-29
    Average 90 stars, based on 1 article reviews
    mneongreen coding sequences - by Bioz Stars, 2026-11
    90/100 stars

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    Isolation:

    Article Title: The flavohemoglobin Yhb1 is a new interacting partner of the heme transporter Str3.
    Article Snippet: Subsequently, the TAP, Cherry, mNeonGreen, and VN coding sequences were isolated by PCR from pJK- 1478NTAPfep1+ (Pelletier et al., 2005), pJK148mfc1+- Cherry (Beaudoin et al., 2011), pFA6a- NeonGreen- KanMX6 (Addgene plasmid 129099), and pSP1ctr5+- VN (Ioannoni et al., 2010), respectively.

    Polymerase Chain Reaction:

    Article Title: The flavohemoglobin Yhb1 is a new interacting partner of the heme transporter Str3.
    Article Snippet: Subsequently, the TAP, Cherry, mNeonGreen, and VN coding sequences were isolated by PCR from pJK- 1478NTAPfep1+ (Pelletier et al., 2005), pJK148mfc1+- Cherry (Beaudoin et al., 2011), pFA6a- NeonGreen- KanMX6 (Addgene plasmid 129099), and pSP1ctr5+- VN (Ioannoni et al., 2010), respectively.

    Plasmid Preparation:

    Article Title: The flavohemoglobin Yhb1 is a new interacting partner of the heme transporter Str3.
    Article Snippet: Subsequently, the TAP, Cherry, mNeonGreen, and VN coding sequences were isolated by PCR from pJK- 1478NTAPfep1+ (Pelletier et al., 2005), pJK148mfc1+- Cherry (Beaudoin et al., 2011), pFA6a- NeonGreen- KanMX6 (Addgene plasmid 129099), and pSP1ctr5+- VN (Ioannoni et al., 2010), respectively.



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    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein <t>mNeonGreen,</t> at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.
    Mneongreen Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mneongreen+coding+sequence/4xmts-mNeonGreen+(Plasmid+%2398876)/pmc11633230-207-22-30
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    mneongreen coding sequences  (Addgene inc)
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    Addgene inc mneongreen coding sequences
    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein <t>mNeonGreen,</t> at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.
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    https://www.bioz.com/product/mneongreen+coding+sequence/mneongreen+coding+sequence/pm38778742-376-3-29
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    mneongreen coding sequence  (Allele Biotechnology)
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    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein <t>mNeonGreen,</t> at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.
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    Image Search Results


    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein mNeonGreen, at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.

    Journal: mBio

    Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis

    doi: 10.1128/mbio.01925-24

    Figure Lengend Snippet: BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein mNeonGreen, at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.

    Article Snippet: The GroES promotor (391 nt, NC_002927.3, 1 041 354–1 041 744) was amplified from chromosomal DNA of B. bronchiseptica RB50, whereas the mNeonGreen coding sequence was amplified from 4xmts-mNeonGreen vector (Addgene item # 98876) using Herculase II Phusion DNA polymerase (Agilent, USA).

    Techniques: Clinical Proteomics, Membrane, Infection, Mutagenesis, Expressing, Sequencing, Bacteria, Comparison, Binding Assay, CellTox Assay, Two Tailed Test, Transfection, Fluorescence, Imaging