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mneongreen coding sequence  (Allele Biotechnology)

 
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    Allele Biotechnology mneongreen coding sequence
    Mneongreen Coding Sequence, supplied by Allele Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mneongreen+coding+sequence/mneongreen+coding+sequence/pm38323924-179-30-34
    Average 90 stars, based on 1 article reviews
    mneongreen coding sequence - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    CRISPR:

    Article Title: Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening
    Article Snippet: .. A patent is in preparation on CRISPR-EATING (RH, AL). mNeonGreen DNA sequence is licensed under MTA from Allele Biotechnology and Pharmaceuticals to the Regents of the University of California. ..

    Sequencing:

    Article Title: Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening
    Article Snippet: .. A patent is in preparation on CRISPR-EATING (RH, AL). mNeonGreen DNA sequence is licensed under MTA from Allele Biotechnology and Pharmaceuticals to the Regents of the University of California. ..

    Article Title: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.
    Article Snippet: To prepare the pmGIT1-GFPhygro vector, the cassette encoding mouse GIT1-GFP was digested out from the mGIT1-GFP-puro vector (30) by XbaI/BstBI and inserted into the pCDH-CMV-MCS-EF1-hygro vector. .. To prepare the lentiviral vector for the expression of C-terminally mNeonGreen-tagged mouse GIT2, the TagRFP tag was digested out from pmGIT2-TagRFP-hygro with BamHI/NotI, resulting in the pmGIT2-hygro vector and the mNeonGreen coding sequence was amplified from the mNeonGreen-EB3-7 plasmid (Allele Biotechnology, San Diego, CA, USA) using forward 5’- ATTAGGATCCATACCGGTCGCCACCATG-3’ and reverse 5’- Frontiers in Immunology 04 ATGCGGCCGCTTACTTGTACAGCTCG-3’ primers. ..

    Article Title: Roles for tubulin recruitment and self-organization by TOG domain arrays in Microtubule plus-end tracking and polymerase
    Article Snippet: .. Coding regions for MT polymerases from Schizosaccharomyces pombe Alp14 (accession: BAA84527.1) or its mutants were inserted into a T7 bacterial expression vectors as either a C-terminal Gly-Ser linker (single amino acid code: GSGS) followed by mNeonGreen coding sequence (Allele Biotechnology) and 6Xhis-tag, or a C-terminal Gly-Ser linker followed by SNAPf coding sequence (NEB #N9183S) and his-tag ( Supplement 1A). ..

    Article Title: Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays
    Article Snippet: .. The cDNA regions encoding MT polymerase activity from Schizosaccharomyces pombe Alp14 (accession: BAA84527.1) or its mutants were inserted into a T7 bacterial expression vectors as a C-terminal Gly-Ser linker (single amino acid code: GSGS) followed by an mNeonGreen coding sequence (Allele Biotechnology) and a 6Xhis-tag, or a C-terminal Gly-Ser linker followed by the SNAPf coding sequence (NEB #N9183S) and a his-tag (Figure S1A). ..

    other:

    Article Title: Filopodial protrusion driven by density-dependent Ena-TOCA-1 interactions.
    Article Snippet: pET-His-SNAP-TOCA-1 (X. tropicalis; GenBank BC080964), pCS2-HisSNAP-Ena (Xenopus laevis; GenBank BC073107), pET-KCK-VASP (X. laevis; GenBank BC077932) and a plasmid expressing SNAP alone were generated previously (Dobramysl et al., 2021). mNeonGreen coding sequence was supplied by Allele Biotechnology and Pharmaceuticals (Shaner et al., 2013), and pCS2-mNG-Ena and pCS2-mNG alone were generated previously (Urbanc ̌ič et al., 2017).

    Article Title: Filopodial protrusion driven by density-dependent Ena–TOCA-1 interactions
    Article Snippet: pET-His-SNAP-TOCA-1 ( X. tropicalis ; GenBank BC080964 ), pCS2-His-SNAP-Ena ( Xenopus laevis ; GenBank BC073107 ), pET-KCK-VASP ( X. laevis ; GenBank BC077932 ) and a plasmid expressing SNAP alone were generated previously ( ). mNeonGreen coding sequence was supplied by Allele Biotechnology and Pharmaceuticals , and pCS2-mNG-Ena and pCS2-mNG alone were generated previously ( ).

    Plasmid Preparation:

    Article Title: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.
    Article Snippet: To prepare the pmGIT1-GFPhygro vector, the cassette encoding mouse GIT1-GFP was digested out from the mGIT1-GFP-puro vector (30) by XbaI/BstBI and inserted into the pCDH-CMV-MCS-EF1-hygro vector. .. To prepare the lentiviral vector for the expression of C-terminally mNeonGreen-tagged mouse GIT2, the TagRFP tag was digested out from pmGIT2-TagRFP-hygro with BamHI/NotI, resulting in the pmGIT2-hygro vector and the mNeonGreen coding sequence was amplified from the mNeonGreen-EB3-7 plasmid (Allele Biotechnology, San Diego, CA, USA) using forward 5’- ATTAGGATCCATACCGGTCGCCACCATG-3’ and reverse 5’- Frontiers in Immunology 04 ATGCGGCCGCTTACTTGTACAGCTCG-3’ primers. ..

    Expressing:

    Article Title: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.
    Article Snippet: To prepare the pmGIT1-GFPhygro vector, the cassette encoding mouse GIT1-GFP was digested out from the mGIT1-GFP-puro vector (30) by XbaI/BstBI and inserted into the pCDH-CMV-MCS-EF1-hygro vector. .. To prepare the lentiviral vector for the expression of C-terminally mNeonGreen-tagged mouse GIT2, the TagRFP tag was digested out from pmGIT2-TagRFP-hygro with BamHI/NotI, resulting in the pmGIT2-hygro vector and the mNeonGreen coding sequence was amplified from the mNeonGreen-EB3-7 plasmid (Allele Biotechnology, San Diego, CA, USA) using forward 5’- ATTAGGATCCATACCGGTCGCCACCATG-3’ and reverse 5’- Frontiers in Immunology 04 ATGCGGCCGCTTACTTGTACAGCTCG-3’ primers. ..

    Article Title: Roles for tubulin recruitment and self-organization by TOG domain arrays in Microtubule plus-end tracking and polymerase
    Article Snippet: .. Coding regions for MT polymerases from Schizosaccharomyces pombe Alp14 (accession: BAA84527.1) or its mutants were inserted into a T7 bacterial expression vectors as either a C-terminal Gly-Ser linker (single amino acid code: GSGS) followed by mNeonGreen coding sequence (Allele Biotechnology) and 6Xhis-tag, or a C-terminal Gly-Ser linker followed by SNAPf coding sequence (NEB #N9183S) and his-tag ( Supplement 1A). ..

    Article Title: Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays
    Article Snippet: .. The cDNA regions encoding MT polymerase activity from Schizosaccharomyces pombe Alp14 (accession: BAA84527.1) or its mutants were inserted into a T7 bacterial expression vectors as a C-terminal Gly-Ser linker (single amino acid code: GSGS) followed by an mNeonGreen coding sequence (Allele Biotechnology) and a 6Xhis-tag, or a C-terminal Gly-Ser linker followed by the SNAPf coding sequence (NEB #N9183S) and a his-tag (Figure S1A). ..

    Amplification:

    Article Title: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.
    Article Snippet: To prepare the pmGIT1-GFPhygro vector, the cassette encoding mouse GIT1-GFP was digested out from the mGIT1-GFP-puro vector (30) by XbaI/BstBI and inserted into the pCDH-CMV-MCS-EF1-hygro vector. .. To prepare the lentiviral vector for the expression of C-terminally mNeonGreen-tagged mouse GIT2, the TagRFP tag was digested out from pmGIT2-TagRFP-hygro with BamHI/NotI, resulting in the pmGIT2-hygro vector and the mNeonGreen coding sequence was amplified from the mNeonGreen-EB3-7 plasmid (Allele Biotechnology, San Diego, CA, USA) using forward 5’- ATTAGGATCCATACCGGTCGCCACCATG-3’ and reverse 5’- Frontiers in Immunology 04 ATGCGGCCGCTTACTTGTACAGCTCG-3’ primers. ..

    Activity Assay:

    Article Title: Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays
    Article Snippet: .. The cDNA regions encoding MT polymerase activity from Schizosaccharomyces pombe Alp14 (accession: BAA84527.1) or its mutants were inserted into a T7 bacterial expression vectors as a C-terminal Gly-Ser linker (single amino acid code: GSGS) followed by an mNeonGreen coding sequence (Allele Biotechnology) and a 6Xhis-tag, or a C-terminal Gly-Ser linker followed by the SNAPf coding sequence (NEB #N9183S) and a his-tag (Figure S1A). ..



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    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein <t>mNeonGreen,</t> at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.
    Mneongreen Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mneongreen coding sequences  (Addgene inc)
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    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein <t>mNeonGreen,</t> at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.
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    mneongreen coding sequence  (Allele Biotechnology)
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    Allele Biotechnology mneongreen coding sequence
    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein <t>mNeonGreen,</t> at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.
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    Image Search Results


    BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein mNeonGreen, at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.

    Journal: mBio

    Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis

    doi: 10.1128/mbio.01925-24

    Figure Lengend Snippet: BteA-induced cell death is characterized by fragmentation of the endoplasmatic reticulum and mitochondrial networks. ( A ) Time lapse analysis of morphological changes and plasma membrane permeabilization in Hela cells. Hela cells were infected with B. bronchiseptica wild-type ( Bb WT) and Bb Δ bteA mutant ( Bb Δ bteA ), expressing the fluorescent protein mNeonGreen, at MOI of 10:1 in the presence of propidium iodide (5 µg/mL). A sequence of time lapse images is shown. Bright field, gray; bacteria, cyan; propidium iodide, magenta. Scale bar, 20 µm. Data are representative of three independent experiments. ( B and C ) Comparison of B. bronchiseptica and B. pertussis cytotoxicity against HeLa cells. HeLa cells were infected with B. bronchiseptica ( B ) or B. pertussis ( C ) wild-type strains and their mutant derivatives at the indicated MOI. Plasma membrane permeabilization was determined using the fluorescent DNA binding dye CellTox Green. For B. bronchiseptica , infections were conducted both in the presence (gly+) and absence of glycine (5 mM). Asterisks indicate a statistically significant difference ( P < 0.05, unpaired two-tailed t -test) between Bb WT and Bb WT gly+ infection samples at 4 h. Data represent the mean ± SEM of a representative experiment out of 2 performed in technical triplicate. ( D ) Visualization of cellular structures. Hela cells were transfected to express fluorescent proteins tagged with localization signals for endoplasmatic reticulum (ER) and mitochondria (mito). One hour after infection with Bb WT and Bb Δ bteA mutant at MOI 50:1 , cells were fixed and analyzed by fluorescence imaging. ER, yellow; mitrochondria, magenta. Scale bar, 20 µm. The shown micrographs are representative of two independent experiments from which the organelle morphology was scored. Analysis was performed on at least 100 cells per experiment and condition and is plotted as morphology % ± SEM. Asterisks indicate statistically significant differences ( P < 0.05, unpaired two-tailed t -test) between the % of fragmented ER or mito compared with uninfected cells.

    Article Snippet: The GroES promotor (391 nt, NC_002927.3, 1 041 354–1 041 744) was amplified from chromosomal DNA of B. bronchiseptica RB50, whereas the mNeonGreen coding sequence was amplified from 4xmts-mNeonGreen vector (Addgene item # 98876) using Herculase II Phusion DNA polymerase (Agilent, USA).

    Techniques: Clinical Proteomics, Membrane, Infection, Mutagenesis, Expressing, Sequencing, Bacteria, Comparison, Binding Assay, CellTox Assay, Two Tailed Test, Transfection, Fluorescence, Imaging