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quantikine elisa kits  (R&D Systems)


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    Structured Review

    R&D Systems quantikine elisa kits
    Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp2/Total+MMP-2+Quantikine+ELISA+Kit/pmc07948871-159-8-11
    Average 95 stars, based on 282 article reviews
    quantikine elisa kits - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Protein Concentration:

    Article Title: siRNA-mediated inhibition of NTT-MMP-2 reduces oxidative stress and apoptotic signaling in an ex vivo model of ischemia/reperfusion injury.
    Article Snippet: .. MMP-2 protein concentration analysis 180 MMP-2 protein concentrations in mitochondrial and cytoplasmic fractions was quantitatively measured using the 181 Quantikine ELISA Assay for Total MMP-2 (R&D Systems, Minneapolis, Minnesota, USA) following the manufacturer’s 182 instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: siRNA-mediated inhibition of NTT-MMP-2 reduces oxidative stress and apoptotic signaling in an ex vivo model of ischemia/reperfusion injury.
    Article Snippet: .. MMP-2 protein concentration analysis 180 MMP-2 protein concentrations in mitochondrial and cytoplasmic fractions was quantitatively measured using the 181 Quantikine ELISA Assay for Total MMP-2 (R&D Systems, Minneapolis, Minnesota, USA) following the manufacturer’s 182 instructions. ..

    Article Title: Ivaltinostat combination therapy for treating pancreatic cancer
    Article Snippet: .. ELISA Analysis: Serological marker measurement was performed using ELISA kits according to the manufacturer's protocols: IL4 (D4050), TIMP1 (DTM100), IL3 (D3000), ILIA (DLA50), PDGFB (DBB00), TEK (or TIE2, DTE200), PECAM1 (DCD310), MMP2 (MMP200), pro-MMP10 (DM1000), KLK3/PSA (DKK300), free IGFBP1 (DGB100), CX3CL1 (DCX310), IL19 (D1900) (all R&D SYSTEMS); and YAP (LifeSpan BioSciences, Inc., LS-F49700). ..

    Article Title: Optimal Switching Antiplatelet Regimen in Patients with Ticagrelor to a Thienopyridine in Korean Patients (SWAPT-K Study).
    Article Snippet: .. Circulating cytokines, including tumor necrosis factor-α (TNF-α, BMS223-4, Invitrogen, Waltham, MA, USA), MMP-2 (MMP200, R&D Systems, Minneapolis, MN, USA), and MMP-9 (DMP200, R&D Systems, Minneapolis, MN, USA), were quantified using enzymelinked immunosorbent assay (ELISA) kits, following the manufacturer’s instructions. ..

    Article Title: Stilbene-based strategies for the management of endometriosis: targeting cell adhesion, migration, and ECM-driven invasion
    Article Snippet: .. The concentration of matrix metalloproteinase 2 (MMP-2) in the cell culture supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) - Total MMP-2 Quantikine ELISA (#MMP200) from R&D Systems (Minneapolis, MN, USA), with an assay range of 0.5–32 ng/mL, according to the supplier's standard procedure. .. The results were normalized to μg of cellular protein content, which was quantified using the Pierce® BCA Protein Assay Kit from Thermo Fisher Scientific (Waltham, MA, USA) according to the manufacturer's protocol.

    other:

    Article Title: Cisplatin-incorporating gelatin-coated gadolinium oxide nanoparticles for cancer theranostics.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Diethylene glycol (DEG, 99%) Nacalai Tesque Cat# 12114-05 Acetone (99%) Nacalai Tesque Cat# 00309-93 Ethanol (99.5%) Nacalai Tesque Cat# 14712-63 Phosphoric acid (85%) Nacalai Tesque Cat# 27618-55 2.5 g/L trypsin – 1 mM EDTA solution Nacalai Tesque Cat# 32777-44 Dulbecco’s phosphate‒buffered saline (PBS) Nacalai Tesque Cat# 14249-24 Cell counting reagent SF Nacalai Tesque Cat# 07553-44 Gadolinium nitrate hexahydrate (Gd(NO 3 ) 3 ٠6H 2 O, 99.5%) Fujifilm Wako Cat# 070-02682 Sodium hydroxide (97.0%) Nacalai Tesque Cat# 31511-05 Isoflurane (98.0%) Fujifilm Wako Cat# 099-06571 Cisplatin (CDDP) Fujifilm Wako Cat# 033-20091 DMEM (High Glucose) containing L‒glutamine, phenol red, and sodium pyruvate Fujifilm Wako Cat# 043-30085 Recombinant human matrix metalloproteinase‒2 (MMP‒2) Sigma–Aldrich Cat# PF023 2–[4–(2–hydroxyl–ethyl)–1–piperazinyl] –ethanesulfonic acid (HEPES, 99%) Sigma–Aldrich Cat# H3375-100g HEPES sodium salt (HEPES–Na, 99%) Sigma–Aldrich Cat# H7006-25g Penicillin-Streptomycin Sigma–Aldrich Cat# P4333-100 ML Human recombinant tissue inhibitor of metalloproteinase‒1 expressed in E. coli (TIMP‒1) Sigma–Aldrich Cat# SRP3173 Human recombinant tissue inhibitor of metalloproteinase‒2 expressed in E. coli (TIMP‒2) Sigma–Aldrich Cat# SRP3174 Fetal bovine serum (FBS) Serana Cat# S-FBS-MX-015 Gelatin (Mw: 99,000) Nitta Gelatin Cat# BM-PS1029 Platinum Standard Solution (1000 ppm) Fujifilm Wako Cat# 162-29691 Gadolinium Standard Solution (1000 ppm) Fujifilm Wako Cat# 070-06761 Medetomidine hydrochloride (Domitor®) Nippon Zenyaku Kogyo CAS# 86347-15-1 Midazolam Fuji Pharma Cat# 135-13791 Butorphanol tartrate (Vetorphale®) Meiji Animal Health CAS# 58786-99-5 Critical commercial assays Total MMP-2 Quantikine ELISA Kit R&D Systems Cat# MMP200 LEGEND MAX TM Human MMP-9 ELISA Kit BioLegend Cat# 444907 LabAssay TM ALT Fujifilm Wako Cat# 293-97501 LabAssay TM AST Fujifilm Wako Cat# 299-97601 LabAssay TM Creatinine Fujifilm Wako Cat# 291-93901 Experimental models: Cell lines HeLa cells RIKEN BRC Cat# RCB3680 MCF-7 cells ATCC Cat# HTB-22 A549 cells ATCC Cat# CCL-185 HEK293 cells JCRB Cat# JCRB0607 L929 cells ATCC Cat# CCL-1 Experimental models: Organisms/strains Mouse: BALB/c nude mice (Slc-nu/nu) Japan SLC Inc. N/A (Continued on next page) iScience 29, 115010, March 20, 2026 e1

    Article Title: Age-related hearing loss in healthy older adults is associated with arterial stiffening and higher aortic systolic blood pressure: potential role of inflammation
    Article Snippet: Inflammatory markers included matrix metalloproteinase-2 (MMP-2) (R&D Systems, Cat. #MMP200), resistin (R&D Systems, Cat. #DRSN00), and vaspin (R&D Systems, Cat. #DSA120).

    Article Title: Correlations of retinal morphology with clinical symptoms, blood-brain barrier, and brain structure in patients with Alzheimer's disease.
    Article Snippet: Background: Patients with Alzheimer’s disease (AD) displayed abnormal retinal morphology; however, its potential associations with clinical symptoms, damage of the blood-brain barrier (BBB), and brain structure are unclear.. Objective: To investigate the correlations of retinal morphology with clinical symptoms, BBB damage, and brain structure in AD patients.. Methods: In 97 patients with mild cognitive impairment due to AD (AD-MCI) and dementia due to AD (AD-D), retinal morphology, clinical symptoms, BBB variables in cerebrospinal fluid, and brain structure variables, including medial temporal atrophy (MTA), global cortical atrophy (GCA), and white matter hyperintensities scores were evaluated.

    Marker:

    Article Title: Ivaltinostat combination therapy for treating pancreatic cancer
    Article Snippet: .. ELISA Analysis: Serological marker measurement was performed using ELISA kits according to the manufacturer's protocols: IL4 (D4050), TIMP1 (DTM100), IL3 (D3000), ILIA (DLA50), PDGFB (DBB00), TEK (or TIE2, DTE200), PECAM1 (DCD310), MMP2 (MMP200), pro-MMP10 (DM1000), KLK3/PSA (DKK300), free IGFBP1 (DGB100), CX3CL1 (DCX310), IL19 (D1900) (all R&D SYSTEMS); and YAP (LifeSpan BioSciences, Inc., LS-F49700). ..

    Concentration Assay:

    Article Title: Stilbene-based strategies for the management of endometriosis: targeting cell adhesion, migration, and ECM-driven invasion
    Article Snippet: .. The concentration of matrix metalloproteinase 2 (MMP-2) in the cell culture supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) - Total MMP-2 Quantikine ELISA (#MMP200) from R&D Systems (Minneapolis, MN, USA), with an assay range of 0.5–32 ng/mL, according to the supplier's standard procedure. .. The results were normalized to μg of cellular protein content, which was quantified using the Pierce® BCA Protein Assay Kit from Thermo Fisher Scientific (Waltham, MA, USA) according to the manufacturer's protocol.

    Cell Culture:

    Article Title: Stilbene-based strategies for the management of endometriosis: targeting cell adhesion, migration, and ECM-driven invasion
    Article Snippet: .. The concentration of matrix metalloproteinase 2 (MMP-2) in the cell culture supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) - Total MMP-2 Quantikine ELISA (#MMP200) from R&D Systems (Minneapolis, MN, USA), with an assay range of 0.5–32 ng/mL, according to the supplier's standard procedure. .. The results were normalized to μg of cellular protein content, which was quantified using the Pierce® BCA Protein Assay Kit from Thermo Fisher Scientific (Waltham, MA, USA) according to the manufacturer's protocol.



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    ABclonal Biotechnology mmp 2
    In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix <t>metalloproteinases</t> <t>MMP-2</t> and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.
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    Image Search Results


    In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix metalloproteinases MMP-2 and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.

    Journal: Materials Today Bio

    Article Title: Human iPSC-EV-loaded nanofiber stent coatings accelerate vascular repair by enhancing EGFR/HIF-1α signaling and suppressing ROCK1-mediated remodeling

    doi: 10.1016/j.mtbio.2026.103564

    Figure Lengend Snippet: In vivo evaluation of re-endothelialization and inhibition of intimal hyperplasia following stent implantation. (A–E) SEM images of the luminal surface treated with EV-loaded (A, B) or DPBS-loaded (D, E) nanofibrous membranes. The EV-treated group exhibited a continuous, smooth, and intact endothelial layer (A, B), whereas the DPBS group displayed incomplete coverage with substantial endothelial disruption (D, E). (C, F) H&E-stained cross-sections revealed the extent of neointimal formation above the stent struts (black arrows). The EV group (C) demonstrated significantly reduced intimal thickening compared with the pronounced hyperplasia observed in the DPBS group (F). (G–H) Statistical analysis confirmed that EV treatment significantly increased endothelial coverage (G) and suppressed intimal hyperplasia thickness (H). (I–K) EV treatment significantly upregulated the expression of the angiogenic factor VEGF (I), the endothelial marker CD31 (J), and the proliferation marker PCNA (K), confirming accelerated vascular repair. (L–N) EV treatment prevented phenotypic switching and matrix accumulation, as evidenced by reduced levels of the contractile/hyperplasia marker α-SMA (L). Furthermore, the EV group showed decreased expression of matrix metalloproteinases MMP-2 and MMP-9 (M) and beneficial modulation of collagen composition, with increased Collagen I and the Collagen I/III ratio (N). (O–P) The EV group exhibited significantly downregulated levels of the profibrotic factor TGF-β1 (O) and the inflammatory cytokine IL-6 (P). N = 5 (G and H), and n = 3 (I-P). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.

    Article Snippet: Proliferation and remodeling markers: PCNA (1:1000, ab29, Abcam), IL-6 (1:1000, A0286, ABclonal), TGF-β1 (1:1000, A2124, ABclonal), MMP-2 (1:1000, A6247, ABclonal), and MMP-9 (1:1000, A2095, ABclonal).

    Techniques: In Vivo, Inhibition, Disruption, Staining, Expressing, Marker