mmp2 Search Results


gene exp mmp2 mm00439505 m1  (Thermo Fisher)
86
Thermo Fisher gene exp mmp2 mm00439505 m1
Gene Exp Mmp2 Mm00439505 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cat no mab902  (R&D Systems)
94
R&D Systems cat no mab902
Cat No Mab902, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mmp2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mmp2
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Mmp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp2/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
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mouse anti human mmp 2  (R&D Systems)
92
R&D Systems mouse anti human mmp 2
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Mouse Anti Human Mmp 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp mmp2 hs00234422 m1  (Thermo Fisher)
99
Thermo Fisher gene exp mmp2 hs00234422 m1
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Gene Exp Mmp2 Hs00234422 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf membrane  (Boster Bio)
95
Boster Bio pvdf membrane
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Pvdf Membrane, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mmp 2  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti mmp 2
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Anti Mmp 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp mmp2 hs01548727 m1  (Thermo Fisher)
99
Thermo Fisher gene exp mmp2 hs01548727 m1
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Gene Exp Mmp2 Hs01548727 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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rat elisa kits  (Cell Applications Inc)
90
Cell Applications Inc rat elisa kits
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Rat Elisa Kits, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mmp 2 sirna  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mmp 2 sirna
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Mmp 2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mmp 2  (Proteintech)
96
Proteintech mmp 2
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Mmp 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human mmp 2 elisa kit  (R&D Systems)
99
R&D Systems human mmp 2 elisa kit
Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. <t>MMP2</t> was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9
Human Mmp 2 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. MMP2 was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9

Journal: Nature cell biology

Article Title: Reprogramming of the tumour microenvironment by stromal PTEN-regulated miR-320.

doi: 10.1038/ncb2396

Figure Lengend Snippet: Figure 4 miR-320 regulates the secretome of MMFs. (a) Pten+/+ and Pten−/−MMFs were left untreated (lanes 1 and 2) or were transiently transfected with miR negative control (NC), miR-320 precursors (320) or anti-miR-320 precursor (anti-320), respectively. Conditioned media were examined by Western blotting with antibodies against the indicated proteins; thrombospondin 2 (THBS2) serves as an internal control. (b) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs expressing siRNA negative control (NC) (n = 4) or siRNA against Mmp9 (n = 4). MMP9 downregulation was verified by Western blot using MMP9 antibody. MMP2 was used as a loading control. Scale bars, 200 µm. The insets in all panels are magnified ×2.5. Quantification of migratory zones (indicated by arrows) expressed as mean area of migration ±s.d., ∗P < 0.01 (bar graphs). (c) Representative images of DB7 mammospheres in the presence of conditioned medium from Pten−/−MMFs precleared with IgG (n = 4) or IgG precoupled with α-MMP9 (n = 4). Western blots using MMP9

Article Snippet: Antibodies used were as follows: LOXL2, MMP2, EMILIN2 (1:500, Santa Cruz Biotechnology), THBS1, THBS2, SFRP1 (1:1,000, R&D Systems), ETS2 (1:1,000, rabbit polyclonal affinity purified), MMP9 (1:1,000, Abcam), BMP1 (1:500, Oncogene), β-actin, α-tubulin (1:10,000, Sigma Aldrich), CTSB, PTEN, AKT, P-AKTS473, JNK, P-JNKT183/Y185, ERK and P-ERKT202/Y204 (1:1,000, Cell Signaling).

Techniques: Transfection, Negative Control, Western Blot, Control, Expressing, Migration