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micrornas microarray profiling  (LC Sciences)

 
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    LC Sciences micrornas microarray profiling
    Role of miR-200a in the control of St3gal3 and St3gal4 in murine primary hepatocytes. ( A ) Fold increase of miR-200a in neonate vs . adult liver was determined in a <t>miRNA</t> array (chip 1 and chip 2) and validated by qRT-PCR in livers from 5 adult and 14 neonate mice. ( B ) Primary hepatocytes from adult mice were transfected with precursor molecules for miR-200a and miR-17-3p and with a scrambled oligonucleotide at 100 nmol/L. After 48 h, total RNA was purified and levels of St3gal3 and St3gal4 were measured by qRT-PCR and normalized with respect to β-actin mRNA. The normalized data were expressed as changes relative to the data of the cells transfected with scrambled pre-miR and set as 100%. Differences were analyzed by means of Student's t test. Statistical significance was taken as *p<0.05, **p<0.01, NS: not statistically significant. The data shown are expressed as mean ± SD, representative of four independent experiments with three replicates each.
    Micrornas Microarray Profiling, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microrna+microarray+profiling+system/micrornas+microarray+profiling/pmc03674942-40-0-26
    Average 90 stars, based on 1 article reviews
    micrornas microarray profiling - by Bioz Stars, 2026-10
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    1) Product Images from "Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a"

    Article Title: Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

    Journal: Journal of Biomedical Science

    doi: 10.1186/1423-0127-20-29

    Role of miR-200a in the control of St3gal3 and St3gal4 in murine primary hepatocytes. ( A ) Fold increase of miR-200a in neonate vs . adult liver was determined in a miRNA array (chip 1 and chip 2) and validated by qRT-PCR in livers from 5 adult and 14 neonate mice. ( B ) Primary hepatocytes from adult mice were transfected with precursor molecules for miR-200a and miR-17-3p and with a scrambled oligonucleotide at 100 nmol/L. After 48 h, total RNA was purified and levels of St3gal3 and St3gal4 were measured by qRT-PCR and normalized with respect to β-actin mRNA. The normalized data were expressed as changes relative to the data of the cells transfected with scrambled pre-miR and set as 100%. Differences were analyzed by means of Student's t test. Statistical significance was taken as *p<0.05, **p<0.01, NS: not statistically significant. The data shown are expressed as mean ± SD, representative of four independent experiments with three replicates each.
    Figure Legend Snippet: Role of miR-200a in the control of St3gal3 and St3gal4 in murine primary hepatocytes. ( A ) Fold increase of miR-200a in neonate vs . adult liver was determined in a miRNA array (chip 1 and chip 2) and validated by qRT-PCR in livers from 5 adult and 14 neonate mice. ( B ) Primary hepatocytes from adult mice were transfected with precursor molecules for miR-200a and miR-17-3p and with a scrambled oligonucleotide at 100 nmol/L. After 48 h, total RNA was purified and levels of St3gal3 and St3gal4 were measured by qRT-PCR and normalized with respect to β-actin mRNA. The normalized data were expressed as changes relative to the data of the cells transfected with scrambled pre-miR and set as 100%. Differences were analyzed by means of Student's t test. Statistical significance was taken as *p<0.05, **p<0.01, NS: not statistically significant. The data shown are expressed as mean ± SD, representative of four independent experiments with three replicates each.

    Techniques Used: Control, Quantitative RT-PCR, Transfection, Purification

    Related Articles

    Microarray:

    Article Title: Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a
    Article Snippet: The RNA concentration and 260/280 ratio were determined by using NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE) and RNA integrity was verified by lab-on-chip technology using the Experion automated electrophoresis system (Bio-Rad Laboratories, Madrid, Spain). .. MicroRNAs microarray profiling was performed using total RNA extracted from the liver from one adult mouse (day +50) and one neonate mouse (day +1) using the LC Sciences technology (LC Sciences, Houston, TX). ..



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    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Journal: Cell Death & Disease

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

    doi: 10.1038/s41419-024-06744-0

    Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.

    Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition

    microRNA and gene expression microarray datasets related to idiopathic pulmonary fibrosis.

    Journal: Mediators of Inflammation

    Article Title: Analysis of Microarray-Identified Genes and MicroRNAs Associated with Idiopathic Pulmonary Fibrosis

    doi: 10.1155/2017/1804240

    Figure Lengend Snippet: microRNA and gene expression microarray datasets related to idiopathic pulmonary fibrosis.

    Article Snippet: Microarray data from IPF-related microRNA and mRNA expression profiles were retrieved and downloaded from the National Center for Biotechnology Information (NCBI) GEO database ( http://www.ncbi.nlmNih.gov/geo ).

    Techniques: Expressing, Microarray