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microarray analysis for gene and microrna profiling  (SeqWright)

 
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    Structured Review

    SeqWright microarray analysis for gene and microrna profiling
    Microarray Analysis For Gene And Microrna Profiling, supplied by SeqWright, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microrna+microarray+profiling+system/microarray+analysis/pmc04303568-108-3-10
    Average 90 stars, based on 1 article reviews
    microarray analysis for gene and microrna profiling - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: MicroRNA 29 Targets Nuclear Factor- κ B–Repressing Factor and Claudin 1 to Increase Intestinal Permeability
    Article Snippet: Cell Culture Human colonic epithelial cells (FHC) and small intestinal epithelial cells (ATCC CCL-241; FHs74Int) (CRL-1831, ATCC, Manassas, VA) were cultured as described previously. .. 10 Microarray Analysis Microarray analysis for gene and microRNA profiling was performed in SeqWright and LC Sciences (Houston, TX) as described previously. ..

    Article Title: Cell type-dependent Erk-Akt pathway crosstalk regulates the proliferation of fetal neural progenitor cells
    Article Snippet: .. Gene expression microarray analysis was done by SeqWright (SeqWright, Houston, TX, USA). .. Total RNA was extracted from cells using a EZ1 RNA Cell Mini Kit (Qiagen, Valencia, CA, USA).

    Article Title: TMEM176A and TMEM176B Are Candidate Regulators of Inhibition of Dendritic Cell Maturation and Function after Chronic Spinal Cord Injury
    Article Snippet: Two micrograms of RNA obtained from each sample were reverse transcribed into complementary DNA (cDNA) using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Microarray analysis was performed by SeqWright, Inc. (Houston, TX). .. Complementary DNA was hybridized to HG-U133A Plus 2.0 GeneChip oligonucleotide arrays (Affymetrix, Santa Clara, CA), each of which contains 54,675 sets of oligonucleotide probes targeting approximately 38,000 human genes.

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: .. At SeqWright, we help the researcher to overcome these hurdles through a variety of service offerings, including copy number determination of DNA or RNA targets, allelic discrimination/ SNP genotyping, microarray validation, microRNA analysis, biodistribution studies of gene therapy products, microbial detection and residual DNA analysis, and transgenic mouse genotyping. ..

    Article Title: Global identification of transcriptional regulators of pluripotency and differentiation in embryonic stem cells
    Article Snippet: All samples were analysed on a LSRII flow cytometer (Becton Dickison). .. Total RNA from control shRNA- and knockdown shRNA-transfected cells were sent to Seqwright for microarray experiment. .. The Human U133 Plus 2.0 array (Affymetrix) was used.

    Article Title: MicroRNA 29 Targets Nuclear Factor- κ B–Repressing Factor and Claudin 1 to Increase Intestinal Permeability
    Article Snippet: Human colonic epithelial cells (FHC) and small intestinal epithelial cells (ATCC CCL-241; FHs74Int) (CRL-1831, ATCC, Manassas, VA) were cultured as described previously. .. Microarray analysis for gene and microRNA profiling was performed in SeqWright and LC Sciences (Houston, TX) as described previously. ..

    Article Title: Kismet Positively Regulates Glutamate Receptor Localization and Synaptic Transmission at the Drosophila Neuromuscular Junction
    Article Snippet: .. Microarray analysis was performed by Seqwright (Houston, Tx). ..

    Article Title: TMEM176A and TMEM176B Are Candidate Regulators of Inhibition of Dendritic Cell Maturation and Function after Chronic Spinal Cord Injury
    Article Snippet: Two micrograms of RNA obtained from each sample were reverse transcribed into complementary DNA (cDNA) using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Microarray analysis Microarray analysis was performed by SeqWright, Inc. (Houston, TX). .. Complementary DNA was hybridized to HG-U133A Plus 2.0 GeneChip oligonucleotide arrays (Affymetrix, Santa Clara, CA), each of which contains 54,675 sets of oligonucleotide probes targeting approximately 38,000 human genes.

    Gene Expression:

    Article Title: Cell type-dependent Erk-Akt pathway crosstalk regulates the proliferation of fetal neural progenitor cells
    Article Snippet: .. Gene expression microarray analysis was done by SeqWright (SeqWright, Houston, TX, USA). .. Total RNA was extracted from cells using a EZ1 RNA Cell Mini Kit (Qiagen, Valencia, CA, USA).

    Biomarker Discovery:

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: .. At SeqWright, we help the researcher to overcome these hurdles through a variety of service offerings, including copy number determination of DNA or RNA targets, allelic discrimination/ SNP genotyping, microarray validation, microRNA analysis, biodistribution studies of gene therapy products, microbial detection and residual DNA analysis, and transgenic mouse genotyping. ..

    Transgenic Assay:

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: .. At SeqWright, we help the researcher to overcome these hurdles through a variety of service offerings, including copy number determination of DNA or RNA targets, allelic discrimination/ SNP genotyping, microarray validation, microRNA analysis, biodistribution studies of gene therapy products, microbial detection and residual DNA analysis, and transgenic mouse genotyping. ..

    Control:

    Article Title: Global identification of transcriptional regulators of pluripotency and differentiation in embryonic stem cells
    Article Snippet: All samples were analysed on a LSRII flow cytometer (Becton Dickison). .. Total RNA from control shRNA- and knockdown shRNA-transfected cells were sent to Seqwright for microarray experiment. .. The Human U133 Plus 2.0 array (Affymetrix) was used.

    shRNA:

    Article Title: Global identification of transcriptional regulators of pluripotency and differentiation in embryonic stem cells
    Article Snippet: All samples were analysed on a LSRII flow cytometer (Becton Dickison). .. Total RNA from control shRNA- and knockdown shRNA-transfected cells were sent to Seqwright for microarray experiment. .. The Human U133 Plus 2.0 array (Affymetrix) was used.

    Knockdown:

    Article Title: Global identification of transcriptional regulators of pluripotency and differentiation in embryonic stem cells
    Article Snippet: All samples were analysed on a LSRII flow cytometer (Becton Dickison). .. Total RNA from control shRNA- and knockdown shRNA-transfected cells were sent to Seqwright for microarray experiment. .. The Human U133 Plus 2.0 array (Affymetrix) was used.



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    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The <t>microRNA</t> profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
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    SeqWright microarray analysis for gene and microrna profiling
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    Image Search Results


    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Journal: Cell Death & Disease

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

    doi: 10.1038/s41419-024-06744-0

    Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.

    Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition

     microRNA  and gene expression  microarray  datasets related to idiopathic pulmonary fibrosis.

    Journal: Mediators of Inflammation

    Article Title: Analysis of Microarray-Identified Genes and MicroRNAs Associated with Idiopathic Pulmonary Fibrosis

    doi: 10.1155/2017/1804240

    Figure Lengend Snippet: microRNA and gene expression microarray datasets related to idiopathic pulmonary fibrosis.

    Article Snippet: Microarray data from IPF-related microRNA and mRNA expression profiles were retrieved and downloaded from the National Center for Biotechnology Information (NCBI) GEO database ( http://www.ncbi.nlmNih.gov/geo ).

    Techniques: Expressing, Microarray