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microarray-based transcriptome-wide mrna expression levels  (Scherf GmbH)

 
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    Structured Review

    Scherf GmbH microarray-based transcriptome-wide mrna expression levels
    Meta-data of genes shown in the cluster analysis of whose <t> mRNA </t> expression correlated with log 10 IC 50 -values of vitamin C in the NCI tumor cell line panel .
    Microarray Based Transcriptome Wide Mrna Expression Levels, supplied by Scherf GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray-based+mrna/mrna+microarray+hybridization/pmc05288649-285-22-31
    Average 90 stars, based on 1 article reviews
    microarray-based transcriptome-wide mrna expression levels - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa —in Cancer Cells"

    Article Title: Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa —in Cancer Cells

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00038

    Meta-data of genes shown in the cluster analysis of whose  mRNA  expression correlated with log 10 IC 50 -values of vitamin C in the NCI tumor cell line panel .
    Figure Legend Snippet: Meta-data of genes shown in the cluster analysis of whose mRNA expression correlated with log 10 IC 50 -values of vitamin C in the NCI tumor cell line panel .

    Techniques Used: Expressing, Virus, Transduction, Activity Assay, Binding Assay, Coagulation, Phospho-proteomics, Residue, Sequencing, Membrane, Activation Assay, Control, Ubiquitin Proteomics

    Dendrograms and cluster image map of curcumin obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.
    Figure Legend Snippet: Dendrograms and cluster image map of curcumin obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.

    Techniques Used: Expressing, Microarray

    Dendrograms and cluster image map of vitamin C obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.
    Figure Legend Snippet: Dendrograms and cluster image map of vitamin C obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.

    Techniques Used: Expressing, Microarray

    Related Articles

    other:

    Article Title: Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa —in Cancer Cells
    Article Snippet: We correlated the IC 50 -values expressed on induction by curcumin and AA of 60 tumor cell lines by COMPARE analysis of microarray-based transcriptome-wide mRNA expression levels of these cell lines (Scherf et al., ).

    Article Title: A multigene expression panel for the molecular diagnosis of Barrett's esophagus and Barrett's adenocarcinoma of the esophagus.
    Article Snippet: The remaining genes were selected because they were highly differentially expressed in esophageal adenocarcinoma compared to normal esophagus in a previous microarray study (Scherf et al., 2001).

    Concentration Assay:

    Article Title: Artemisinin derivatives induce iron-dependent cell death (ferroptosis) in tumor cells.
    Article Snippet: Background: Apoptosis and other forms of cell death have been intensively investigated in the past years to explain the mode of action of synthetic anticancer drugs and natural products.. Recently, a new form of cell death emerged, which was termed ferroptosis, because it depends on intracellular iron.. Here, the role of genes involved in iron metabolism and homeostasis for the cytotoxicity of ten artemisinin derivatives have been systematically investigated.

    Microarray:

    Article Title: Artemisinin derivatives induce iron-dependent cell death (ferroptosis) in tumor cells.
    Article Snippet: Background: Apoptosis and other forms of cell death have been intensively investigated in the past years to explain the mode of action of synthetic anticancer drugs and natural products.. Recently, a new form of cell death emerged, which was termed ferroptosis, because it depends on intracellular iron.. Here, the role of genes involved in iron metabolism and homeostasis for the cytotoxicity of ten artemisinin derivatives have been systematically investigated.

    Article Title: Use of RNA amplification in the optimal characterization of global gene expression using cDNA microarrays.
    Article Snippet: RADKA STOYANOVA, JOHN J. UPSON, CHRISTOS PATRIOTIS, ERIC A. ROSS, ELIZABETH P. HENSKE, KETAKI DATTA, BRUCE BOMAN, MARGIE L. CLAPPER, ALFRED G. KNUDSON, AND ALFONSO BELLACOSA* Division of Population Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania Division of Medical Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania Division of Genetics and Preventive Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania

    Article Title: Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.
    Article Snippet: Background: Ziziphus spina-christi (L.) Desf. (Christ’s Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt.. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects.. Nowadays, Z. spina-christi , is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation.

    Article Title: Pharmacogenomics of Cameroonian traditional herbal medicine for cancer therapy.
    Article Snippet: Ethnopharmacological relevance: A socio-economic burden associated with cancers is reported in Africa.. Ethnopharmacological usages such as immune and skin disorders, inflammatory, and others chould be considered when selecting plants used to treat cancer, since these reflect disease states bearing relevance to cancer or a cancer symptoms.. Materials and methods: Documented compounds of Cameroonian medicinal plants were used as keywords in the National Cancer Institute (NCI) database to establish a library of cytotoxic compounds.

    Article Title: Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin.
    Article Snippet: Artemisia annua L. (sweet wormwood, qinhao) has traditionally been used in Chinese medicine.. The isolation of artemisinin from Artemisia annua and its worldwide accepted application in malaria therapy is one of the showcase success stories of phytomedicine during the past decades.. Artemisinin-type compounds are also active towards other protozoal or viral diseases as well as cancer cells in vitro and in vivo.

    Article Title: Discovering causes and cures for cancer from gene expression analysis.
    Article Snippet: Tumorigenesis is governed by a series of complex genetic and epigenetic changes.. Both mechanisms can result in either the silencing or aberrant expression of messages in a cell.. Gene expression profiling techniques such as the serial analysis of gene expression (SAGE) or microarray analysis can provide global overviews of these changes, as well identify key genes and pathways involved in this process.

    Hybridization:

    Article Title: Artemisinin derivatives induce iron-dependent cell death (ferroptosis) in tumor cells.
    Article Snippet: Background: Apoptosis and other forms of cell death have been intensively investigated in the past years to explain the mode of action of synthetic anticancer drugs and natural products.. Recently, a new form of cell death emerged, which was termed ferroptosis, because it depends on intracellular iron.. Here, the role of genes involved in iron metabolism and homeostasis for the cytotoxicity of ten artemisinin derivatives have been systematically investigated.

    Article Title: Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.
    Article Snippet: Background: Ziziphus spina-christi (L.) Desf. (Christ’s Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt.. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects.. Nowadays, Z. spina-christi , is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation.

    Article Title: Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin.
    Article Snippet: Artemisia annua L. (sweet wormwood, qinhao) has traditionally been used in Chinese medicine.. The isolation of artemisinin from Artemisia annua and its worldwide accepted application in malaria therapy is one of the showcase success stories of phytomedicine during the past decades.. Artemisinin-type compounds are also active towards other protozoal or viral diseases as well as cancer cells in vitro and in vivo.

    Gene Expression:

    Article Title: Use of RNA amplification in the optimal characterization of global gene expression using cDNA microarrays.
    Article Snippet: RADKA STOYANOVA, JOHN J. UPSON, CHRISTOS PATRIOTIS, ERIC A. ROSS, ELIZABETH P. HENSKE, KETAKI DATTA, BRUCE BOMAN, MARGIE L. CLAPPER, ALFRED G. KNUDSON, AND ALFONSO BELLACOSA* Division of Population Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania Division of Medical Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania Division of Genetics and Preventive Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania

    Article Title: Discovering causes and cures for cancer from gene expression analysis.
    Article Snippet: Tumorigenesis is governed by a series of complex genetic and epigenetic changes.. Both mechanisms can result in either the silencing or aberrant expression of messages in a cell.. Gene expression profiling techniques such as the serial analysis of gene expression (SAGE) or microarray analysis can provide global overviews of these changes, as well identify key genes and pathways involved in this process.

    Diagnostic Assay:

    Article Title: Use of RNA amplification in the optimal characterization of global gene expression using cDNA microarrays.
    Article Snippet: RADKA STOYANOVA, JOHN J. UPSON, CHRISTOS PATRIOTIS, ERIC A. ROSS, ELIZABETH P. HENSKE, KETAKI DATTA, BRUCE BOMAN, MARGIE L. CLAPPER, ALFRED G. KNUDSON, AND ALFONSO BELLACOSA* Division of Population Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania Division of Medical Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania Division of Genetics and Preventive Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania

    Binding Assay:

    Article Title: Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.
    Article Snippet: Background: Ziziphus spina-christi (L.) Desf. (Christ’s Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt.. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects.. Nowadays, Z. spina-christi , is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation.

    Inhibition:

    Article Title: Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.
    Article Snippet: Background: Ziziphus spina-christi (L.) Desf. (Christ’s Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt.. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects.. Nowadays, Z. spina-christi , is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation.

    Expressing:

    Article Title: Pharmacogenomics of Cameroonian traditional herbal medicine for cancer therapy.
    Article Snippet: Ethnopharmacological relevance: A socio-economic burden associated with cancers is reported in Africa.. Ethnopharmacological usages such as immune and skin disorders, inflammatory, and others chould be considered when selecting plants used to treat cancer, since these reflect disease states bearing relevance to cancer or a cancer symptoms.. Materials and methods: Documented compounds of Cameroonian medicinal plants were used as keywords in the National Cancer Institute (NCI) database to establish a library of cytotoxic compounds.



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    Image Search Results


    Meta-data of genes shown in the cluster analysis of whose  mRNA  expression correlated with log 10 IC 50 -values of vitamin C in the NCI tumor cell line panel .

    Journal: Frontiers in Pharmacology

    Article Title: Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa —in Cancer Cells

    doi: 10.3389/fphar.2017.00038

    Figure Lengend Snippet: Meta-data of genes shown in the cluster analysis of whose mRNA expression correlated with log 10 IC 50 -values of vitamin C in the NCI tumor cell line panel .

    Article Snippet: We correlated the IC 50 -values expressed on induction by curcumin and AA of 60 tumor cell lines by COMPARE analysis of microarray-based transcriptome-wide mRNA expression levels of these cell lines (Scherf et al., ).

    Techniques: Expressing, Virus, Transduction, Activity Assay, Binding Assay, Coagulation, Phospho-proteomics, Residue, Sequencing, Membrane, Activation Assay, Control, Ubiquitin Proteomics

    Dendrograms and cluster image map of curcumin obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.

    Journal: Frontiers in Pharmacology

    Article Title: Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa —in Cancer Cells

    doi: 10.3389/fphar.2017.00038

    Figure Lengend Snippet: Dendrograms and cluster image map of curcumin obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.

    Article Snippet: We correlated the IC 50 -values expressed on induction by curcumin and AA of 60 tumor cell lines by COMPARE analysis of microarray-based transcriptome-wide mRNA expression levels of these cell lines (Scherf et al., ).

    Techniques: Expressing, Microarray

    Dendrograms and cluster image map of vitamin C obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.

    Journal: Frontiers in Pharmacology

    Article Title: Pharmacogenomic Characterization and Isobologram Analysis of the Combination of Ascorbic Acid and Curcumin—Two Main Metabolites of Curcuma longa —in Cancer Cells

    doi: 10.3389/fphar.2017.00038

    Figure Lengend Snippet: Dendrograms and cluster image map of vitamin C obtained by hierarchical cluster analysis of mRNA expression of 40 genes in the NCI cell line panel as analyzed by the Novartis microarray platform . The dendrogram on the left shows the clustering of cell lines and the dendrogram on the top shows the clustering of genes. The cluster image map shows each single mRNA expression value obtained by microarray analysis. The expression values have been normalized and color-coded.

    Article Snippet: We correlated the IC 50 -values expressed on induction by curcumin and AA of 60 tumor cell lines by COMPARE analysis of microarray-based transcriptome-wide mRNA expression levels of these cell lines (Scherf et al., ).

    Techniques: Expressing, Microarray

    Rank correlations between  microarray-  and real-time quantitative PCR–based gene expression

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling

    doi: 10.1073/pnas.1509322112

    Figure Lengend Snippet: Rank correlations between microarray- and real-time quantitative PCR–based gene expression

    Article Snippet: An additional cryosection from each of 16 normal specimens, 13 CIN1/CIN2 specimens, 23 CIN3 specimens, and 16 cancer specimens was used to independently validate the Affymetrix microarray-based mRNA profiling data by RTQ-PCR.

    Techniques: Microarray

    Expression of genes encoding ribosomal proteins and mitochondrial electron transport chain complexes I, III, IV, and V is reduced at the transition from CIN3 to cancer. (A) Twenty-four small and 35 large ribosomal subunit proteins (∼70% of all ribosomal proteins) are expressed at statistically significant reduced levels in cancers compared with precancerous CIN3 lesions. Histograms show measurements in cancers by all 177 Affymetrix microarray probe sets for ribosomal protein-encoding genes, normalized to levels measured in CIN3s. (B) Green dots in the five complexes of the mitochondrial electron transport chain indicate proteins with reduced gene expression in cancers. Box plots (Tukey) show the average distribution of gene expression of all differentially expressed ETC complex I, III, IV, and V components (median levels are in red).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling

    doi: 10.1073/pnas.1509322112

    Figure Lengend Snippet: Expression of genes encoding ribosomal proteins and mitochondrial electron transport chain complexes I, III, IV, and V is reduced at the transition from CIN3 to cancer. (A) Twenty-four small and 35 large ribosomal subunit proteins (∼70% of all ribosomal proteins) are expressed at statistically significant reduced levels in cancers compared with precancerous CIN3 lesions. Histograms show measurements in cancers by all 177 Affymetrix microarray probe sets for ribosomal protein-encoding genes, normalized to levels measured in CIN3s. (B) Green dots in the five complexes of the mitochondrial electron transport chain indicate proteins with reduced gene expression in cancers. Box plots (Tukey) show the average distribution of gene expression of all differentially expressed ETC complex I, III, IV, and V components (median levels are in red).

    Article Snippet: An additional cryosection from each of 16 normal specimens, 13 CIN1/CIN2 specimens, 23 CIN3 specimens, and 16 cancer specimens was used to independently validate the Affymetrix microarray-based mRNA profiling data by RTQ-PCR.

    Techniques: Expressing, Microarray

    Differential expression of estrogen-responsive genes correlates strongly with cervical cancer progression. (A) Hierarchical clustering of 128 specimens based on changes in gene expression measured by 365 Affymetrix probe sets measuring expression of 217 estrogen-responsive genes shows strong correlation with disease progression. (B) Box plots (Tukey) show the distribution of Affymetrix microarray-based gene expression measurements of CDKN2A (p16), ERα, and estrogen-responsive genes GREB1, PGR1, and AR. White and red horizontal lines indicate median expression levels.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling

    doi: 10.1073/pnas.1509322112

    Figure Lengend Snippet: Differential expression of estrogen-responsive genes correlates strongly with cervical cancer progression. (A) Hierarchical clustering of 128 specimens based on changes in gene expression measured by 365 Affymetrix probe sets measuring expression of 217 estrogen-responsive genes shows strong correlation with disease progression. (B) Box plots (Tukey) show the distribution of Affymetrix microarray-based gene expression measurements of CDKN2A (p16), ERα, and estrogen-responsive genes GREB1, PGR1, and AR. White and red horizontal lines indicate median expression levels.

    Article Snippet: An additional cryosection from each of 16 normal specimens, 13 CIN1/CIN2 specimens, 23 CIN3 specimens, and 16 cancer specimens was used to independently validate the Affymetrix microarray-based mRNA profiling data by RTQ-PCR.

    Techniques: Expressing, Microarray