one-color (cy3 fluorochrome) microarray-based gene expression platform  (Agilent technologies)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Hispidin Inhibits Ferroptosis Induced by High Glucose via the miR-15b-5p/GLS2 Axis in Pancreatic Beta Cells

doi: 10.1155/2023/9428241

Figure Lengend Snippet: Hispidin treatment led to the upregulation of miR-15b-5p in PBCs. (a) PBCs treated with hispidin (40 μ M) or DMSO for 24 h; the expression of miRNAs was assayed using a microarray technique. (b) PBCs treated with hispidin (40 μ M) for 24 h; the expression of miR-15b-5p was analyzed by RT-PCR. PBCs treated with DMSO were used as the control. (c) PBCs transfected with the indicated miRNA mimic/inhibitor for 24 h; the expression of miR-802 was and analyzed by RT-PCR. (d)–(i) Under the HG condition, PBCs transfected with the indicated miRNA mimic/inhibitor for 24 h following which the cells were treated with hispidin (40 μ M) for an additional 24 h. Cells cultured under normal conditions were used as the control. (d) Cell viability measured. (e) Cell death measured. (f) ROS levels measured. (g) Fe 2+ levels measured. (h) MDA levels measured. (i) GSH levels measured. Data are representative of at least three independent experiments ( ∗ P < 0.05).

Article Snippet: The miRNA expression analysis was performed based on the Aksomics Technology (China) using the Agilent microarray-based miRNA platform.

Techniques: Expressing, Microarray, Reverse Transcription Polymerase Chain Reaction, Transfection, Cell Culture

Journal: PLoS ONE

Article Title: Global Expression of Molecular Transporters in the Human Vaginal Tract: Implications for HIV Chemoprophylaxis

doi: 10.1371/journal.pone.0077340

Figure Lengend Snippet: The box extends from the 25th to 75th percentiles, with the horizontal line in the box representing the median; whiskers represent the lowest and highest datum. ( A ) Normalized probe intensity data from microarray experiments. ( B ) Normalized RT-qPCR measurements, expressed as 1/[Δ(C t )] such that increased values correspond to increased gene expression.

Article Snippet: Gene expression profiles were measured using the Agilent one-color (Cy3 fluorochrome) microarray-based gene expression platform according to manufacturer's instructions.

Techniques: Microarray, Quantitative RT-PCR, Expressing

Journal: PLoS ONE

Article Title: Global Expression of Molecular Transporters in the Human Vaginal Tract: Implications for HIV Chemoprophylaxis

doi: 10.1371/journal.pone.0077340

Figure Lengend Snippet: Correlation of gene expression for the six ARV membrane transporter genes ( ABCB1 , ABCC1 , ABCC2 , ABCC3 , ABCC4 , ABCG2 ) across microarray and qPCR platforms ( R 2 = 0.70, P < 2.2×10 -16 ).

Article Snippet: Gene expression profiles were measured using the Agilent one-color (Cy3 fluorochrome) microarray-based gene expression platform according to manufacturer's instructions.

Techniques: Expressing, Microarray

Journal: PLoS ONE

Article Title: Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry

doi: 10.1371/journal.pone.0201462

Figure Lengend Snippet: Fold changes obtained by microarray (fill) and by qPCR (hatched) when fed the M diet (dark grey) or the V diet (light grey) for genes involved in sensory perception (R23h versus A22h), immunity (R23h versus AB1h) and for amino acid metabolism (R23h versus A22h).

Article Snippet: Microarray analyses were performed on an Agilent-based microarray platform with 8 X 60 K probes per slide.

Techniques: Microarray

Journal: PLoS ONE

Article Title: Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry

doi: 10.1371/journal.pone.0201462

Figure Lengend Snippet: Correlation between gene expression patterns obtained through real-time PCR and microarray approaches.

Article Snippet: Microarray analyses were performed on an Agilent-based microarray platform with 8 X 60 K probes per slide.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray

Journal: BMC Genomics

Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

doi: 10.1186/1471-2164-12-326

Figure Lengend Snippet: Microarray interplatform analysis . (A) Overlap of unique and named genes shared among the 3 microarray platforms used in this study. The pool of 17070 shared genes was used for further cross-platform analysis. The total numbers of genes for each platform and for all platforms combined are indicated. (B) Overlap of significantly regulated genes at 6 h after EGF treatment considering each of the 3 microarray platforms independently.

Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

Techniques: Microarray

Journal: BMC Genomics

Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

doi: 10.1186/1471-2164-12-326

Figure Lengend Snippet: GSEA analysis on significantly regulated gene sets across microarray platforms . Profile of the Running ES Score & Positions of Gene Set Members on the Rank Ordered List using 6 h EGF treatment data according to each of the three microarray platforms. In each panel, the vertical black lines indicate the position of each of the genes of the tested gene set in the reference data set (ranked by average of the three respective EGF versus control log2ratios of replicate experiments). The green curve plots the ES (enrichment score), which is the running sum of the weighted enrichment score obtained from GSEA software. Within each queried gene set, the farther the position of a gene to the left (red) implies a higher correlation with EGF up-regulated genes in the reference platform, and the farther to the right (blue) implies a higher correlation with genes down-regulated upon EGF treatment in the reference platform. Studied gene sets correspond to lists of up- or down-regulated genes in each platform at 6 h of EGF treatment. Significantly enriched data sets are defined according to GSEA default settings (p < 0.001 and a false discovery rate (FDR) < 0.25). R.L.M = ranked list metric.

Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

Techniques: Microarray, Software

Journal: BMC Genomics

Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

doi: 10.1186/1471-2164-12-326

Figure Lengend Snippet: Microarray versus DGE analysis . (A) Overlap of unique and named genes shared among the 3 microarray platforms and genes detected by DGE. The pool of 14645 shared genes was used for further cross-platform analysis. The total numbers of genes for each platform and for all platforms combined are indicated. (B) Overlap of significantly regulated genes considering the 3 microarray platforms at 6 h after EGF treatment and the genes found regulated after assessing significance by grouping microarray and DGE data in a RankProd analysis. Left panels show up-regulated genes and right panels show down-regulated genes.

Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

Techniques: Microarray

Journal: BMC Genomics

Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

doi: 10.1186/1471-2164-12-326

Figure Lengend Snippet: Correlation between microarrays and Illumina GA-I sequencing . (A) Comparison of estimated log2ratios from DGE ( Y -axis) and the mean of all microarray platforms ( X -axis). We consider only genes that were interrogated using all platforms and genes with a mean number of counts across lanes greater than 0. Genes with counts greater than 32 reads (colored red or green) or less than (black) 32 reads in at least one sample are shown. (Red dots) Genes called differentially expressed based on DGE data at an 10% FDR by RankProd. (Green dots) Genes not called as differentially expressed but above 32 counts. (Inset box) Correlation between technologies is higher when considering genes above the 32 count detection level (0.57) than when all genes are included (0.49). (B-C) Concordance at the top (CAT) plots of the different platforms with the 500 top genes from a reference platform, shown for Agilent in (B) and DGE in (C). See inset box for color codes identifying each platforms compared to the remaining platform used as reference. (D) Correlation plots with regression lines between log2ratios of the five high content platforms measurements (Y-axis) and quantitative real time PCR results using SYBR green assays (X-axis), based on measurements for 21 genes at the 6 h time point (see Additional file , Table S1).

Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

Techniques: Sequencing, Microarray, Real-time Polymerase Chain Reaction, SYBR Green Assay

Journal: BMC Genomics

Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

doi: 10.1186/1471-2164-12-326

Figure Lengend Snippet: Top regulated genes derived from meta-analysis . RankProd analysis of the combination of microarray and Illumina GA-I ultrasequencing data sets. Heatmap of the top 50 up and down-regulated genes detected in all four platforms ordered by Median Fold Change (all have RankProd adjusted p-values < 0.0001). IL11, IL8, PLAUR, ANXA10 and FOS were validated by RT-qPCR showing concordant results (See Additional file , Table S1). The full RankProd matrix from these experiments is accessible in Additional file , Table S5. The list of all 1164 significantly regulated genes (median |FC| > 1.2 and RankProd q-value < 0.05) is given in Additional file , Table S6.

Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

Techniques: Derivative Assay, Microarray, Quantitative RT-PCR