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glycan microarray screening  (Chemie GmbH)

 
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    Chemie GmbH glycan microarray screening
    Glycan Microarray Screening, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+screening/glycan+microarray+screening/pm39888186-185-12-101
    Average 90 stars, based on 1 article reviews
    glycan microarray screening - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Glycoproteomics:

    Article Title: Divergent Chemoenzymatic Synthesis of Sulfated Ganglio-Oligosaccharides for Probing Ligand Requirements of Glycan Binding Proteins.
    Article Snippet: Sulfoglycolipids are an important class of acidic glycosphingolipids that have been implicated in a multitude of biological processes.. Little is known about the interactome of sulfated gangliosides, and it is not well understood how a possible interplay between sialylation and sulfation influences molecular recognition.. Here, we describe a chemoenzymatic strategy that readily provided a panel of twenty-one sulfated and sialylated ganglio-oligosaccharides.

    Microarray:

    Article Title: Divergent Chemoenzymatic Synthesis of Sulfated Ganglio-Oligosaccharides for Probing Ligand Requirements of Glycan Binding Proteins.
    Article Snippet: Sulfoglycolipids are an important class of acidic glycosphingolipids that have been implicated in a multitude of biological processes.. Little is known about the interactome of sulfated gangliosides, and it is not well understood how a possible interplay between sialylation and sulfation influences molecular recognition.. Here, we describe a chemoenzymatic strategy that readily provided a panel of twenty-one sulfated and sialylated ganglio-oligosaccharides.



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    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

    Journal: Communications Medicine

    Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

    doi: 10.1038/s43856-025-00870-2

    Figure Lengend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

    Article Snippet: Binding of p4796kb-derived sera to 3 hits from the HuProt™ microarray screen (HSP90 beta protein, StressMarq Bioscience, SPR-102B; AKR1B10 protein, MyBioSource, MBS203315; PTPRD protein, Acro, PTD-H52H9) and to other propeptides that belong to the calcitonin/CGRP peptide family, including recombinant adrenomedullin (ADM, MyBioSource, MBS2012013), recombinant adrenomedullin 2 (ADM2, MyBioSource, MBS2123890), synthetic amylin (Abcam, ab142398), and recombinant calcitonin (Abcam, ab153793), were further evaluated.

    Techniques: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay