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whole human genome 4 * 44 k microarrays (g4112f) applying for gene expression profiling  (Agilent technologies)


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    Agilent technologies whole human genome 4 * 44 k microarrays (g4112f) applying for gene expression profiling
    Whole Human Genome 4 * 44 K Microarrays (G4112f) Applying For Gene Expression Profiling, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+profiling+of+human+gene+expression/pm36077272-320-7-32
    Average 90 stars, based on 1 article reviews
    whole human genome 4 * 44 k microarrays (g4112f) applying for gene expression profiling - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Computational reassessment of RNA-seq data reveals key genes in active tuberculosis
    Article Snippet: GSE34608 consists of 18 controls and 8 TB samples (GPL6480 platform; Agilent-014850 Whole Human Genome Microarray 4x44K G4112F) [ ].

    Article Title: Clinical Phenotypes of Patients With Systemic Sclerosis With Distinct Molecular Signatures in Skin.
    Article Snippet: Objective.. Systemic sclerosis (SSc) patients are classified according to degree of skin fibrosis (limited and diffuse cutaneous [lc and dc]) and serum autoantibodies.. We undertook the present multicenter study to determine whether intrinsic subset (IS) classification based upon skin gene expression yields additional valuable clinical information.

    Article Title: Bioinformatics analysis revealed the potential crosstalk genes and molecular mechanisms between intracranial aneurysms and periodontitis
    Article Snippet: The mRNA sequencing of the data set GSE54083 was based on GPL4133 Agilent-014850 Whole Human Genome Microarray 4 × 44 K G4112F (Feature Number version).

    Article Title: Discovering the effect of combination of celecoxib and sorafenib on hepatocellular carcinoma
    Article Snippet: The platform used was the Agilent-014850 whole human genome microarray 4 × 44 k G4112F (feature number version).

    Expressing:

    Article Title: M6A-mediated molecular patterns and tumor microenvironment infiltration characterization in nasopharyngeal carcinoma
    Article Snippet: The mRNA expression data of the GSE102349 dataset (Submission date: Aug 08, 2017, Last update date: May 15, 2019, data retrieval: Jan 08, 2022) was generated using Illumina HiSeq 2000, this dataset includes mRNA expression data and clinical information from 113 NPC patients, and 88 cases with progression-free survival (PFS) involved in this study. .. And the mRNA expression data of the GSE53819 dataset (Submission date: Jan 04, 2014, Last update date: Aug 01, 2019, data retrieval: Jan 08, 2022) was generated using Agilent -014,850 Whole Human Genome Microarray 4 × 44K G4112F, and this dataset contained 18 NPC primary tumor tissues and 18 non-cancerous nasopharyngeal tissues. ..

    Generated:

    Article Title: M6A-mediated molecular patterns and tumor microenvironment infiltration characterization in nasopharyngeal carcinoma
    Article Snippet: The mRNA expression data of the GSE102349 dataset (Submission date: Aug 08, 2017, Last update date: May 15, 2019, data retrieval: Jan 08, 2022) was generated using Illumina HiSeq 2000, this dataset includes mRNA expression data and clinical information from 113 NPC patients, and 88 cases with progression-free survival (PFS) involved in this study. .. And the mRNA expression data of the GSE53819 dataset (Submission date: Jan 04, 2014, Last update date: Aug 01, 2019, data retrieval: Jan 08, 2022) was generated using Agilent -014,850 Whole Human Genome Microarray 4 × 44K G4112F, and this dataset contained 18 NPC primary tumor tissues and 18 non-cancerous nasopharyngeal tissues. ..

    Microarray:

    Article Title: M6A-mediated molecular patterns and tumor microenvironment infiltration characterization in nasopharyngeal carcinoma
    Article Snippet: The mRNA expression data of the GSE102349 dataset (Submission date: Aug 08, 2017, Last update date: May 15, 2019, data retrieval: Jan 08, 2022) was generated using Illumina HiSeq 2000, this dataset includes mRNA expression data and clinical information from 113 NPC patients, and 88 cases with progression-free survival (PFS) involved in this study. .. And the mRNA expression data of the GSE53819 dataset (Submission date: Jan 04, 2014, Last update date: Aug 01, 2019, data retrieval: Jan 08, 2022) was generated using Agilent -014,850 Whole Human Genome Microarray 4 × 44K G4112F, and this dataset contained 18 NPC primary tumor tissues and 18 non-cancerous nasopharyngeal tissues. ..

    Article Title: EPAS1 , a hypoxia‐ and f erroptosis‐related gene, promotes malignant behaviour of cervical cancer by ceRNA and super‐enhancer
    Article Snippet: The GSE63514 data set, including 10 normal and 24 cancer samples, was derived from the GPL570 platform of the [HG‐U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array. .. The GSE138080 data set, including 10 normal samples and 10 cancer samples, was derived from the GPL4133 platform of the Agilent‐014850 Whole Human Genome Microarray 4 × 44 K G4112F. ..

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay
    Article Snippet: 200 ng total RNA was transcribed into cDNA with an oligo-dT primer, followed by transcription to cRNA labeled with cyanine 3-CTP (Quick-Amp Labeling Kit, One-color, Agilent). cRNA purification was performed with the RNeasy Mini Kit (Qiagen) and dye incorporation and cRNA yields were measured with the NanoDrop-1000 spectrophotometer (Thermofisher). .. Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a hybridization oven (Agilent) at 65°C for 17 h. After hybridization, DNA microarrays were washed, and slides were immediately scanned with the Microarray Scanner (G2505 B, Agilent) as recommended by Agilent.

    Hybridization:

    Article Title: Resistance to Naïve and Formative Pluripotency Conversion in RSeT Human Embryonic Stem Cells
    Article Snippet: Cy3-labeled cRNAs were measured using the NanoDrop Spectrophotometer. .. Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242, Agilent Technologies) at 65°C for 17 hours at 10 r.p.m. as recommended. .. Slides were scanned using an Agilent DNA microarray compatible scanner, with a one-color scan setting for 4 x 44k array slides.

    Derivative Assay:

    Article Title: EPAS1 , a hypoxia‐ and f erroptosis‐related gene, promotes malignant behaviour of cervical cancer by ceRNA and super‐enhancer
    Article Snippet: The GSE63514 data set, including 10 normal and 24 cancer samples, was derived from the GPL570 platform of the [HG‐U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array. .. The GSE138080 data set, including 10 normal samples and 10 cancer samples, was derived from the GPL4133 platform of the Agilent‐014850 Whole Human Genome Microarray 4 × 44 K G4112F. ..

    Labeling:

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay
    Article Snippet: 200 ng total RNA was transcribed into cDNA with an oligo-dT primer, followed by transcription to cRNA labeled with cyanine 3-CTP (Quick-Amp Labeling Kit, One-color, Agilent). cRNA purification was performed with the RNeasy Mini Kit (Qiagen) and dye incorporation and cRNA yields were measured with the NanoDrop-1000 spectrophotometer (Thermofisher). .. Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a hybridization oven (Agilent) at 65°C for 17 h. After hybridization, DNA microarrays were washed, and slides were immediately scanned with the Microarray Scanner (G2505 B, Agilent) as recommended by Agilent.



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    Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

    Journal: Breast Cancer Research : BCR

    Article Title: Activating transcription factor-2 (ATF2) is a key determinant of resistance to endocrine treatment in an in vitro model of breast cancer

    doi: 10.1186/s13058-020-01359-7

    Figure Lengend Snippet: Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

    Article Snippet: We also performed a microarray gene expression profiling (Illumina Human HT12_v4) to explore alterations in gene expression between MCF7 and TAMRs after ATF2 silencing and confirmed gene expression changes by quantitative RT-PCR.

    Techniques: Biomarker Discovery, Microarray, Transfection, Negative Control, Quantitative RT-PCR, Knockdown