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microarray processing analysis  (Thermo Fisher)


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    Structured Review

    Thermo Fisher microarray processing analysis
    Microarray Processing Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+processing+and+analysis/pm35077654-102-1-0
    Average 90 stars, based on 1 article reviews
    microarray processing analysis - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: In vivo gene expression profile of guided bone regeneration associated with a microrough titanium surface.
    Article Snippet: Objectives: To determine the gene expression profile characteristic of ‘‘guided bone regeneration’’ associated with a microrough titanium surface.. Material and methods: Critical-size calvarial defects were treated with the principle of ‘‘guided bone regeneration,’’ whereby the extracranial barriers were either polished (SMO) or microrough (SLA) titanium disks.. After 7 and 14 days, the contents of the regenerating defect were collected, RNA was extracted and microarray analysis was carried out.

    Article Title: Therapeutic potential of MEK inhibition in acute myelogenous leukemia: rationale for "vertical" and "lateral" combination strategies.
    Article Snippet: In hematological malignancies, constitutive activation of the RAF/MEK/ERK pathway is frequently observed, conveys a poor prognosis, and constitutes a promising target for therapeutic intervention.. Here, we investigated the molecular and functional effects of pharmacological MEK inhibition in cell line models of acute myeloid leukemia (AML) and freshly isolated primary AML samples.. The small-molecule, ATP-non-competitive, MEK inhibitor PD0325901 markedly inhibited ERK phosphorylation and growth of several AML cell lines and approximately 70 % of primary AML samples.

    Article Title: CD52 as a molecular target for immunotherapy to treat acute myeloid leukemia with high EVI1 expression.
    Article Snippet: The antibody against caspase-3 was commercially obtained from Cell Signaling Technologies (Beverly, MA, USA; catalog no. 9251). .. The protocol used for the sample preparation and microarray processing is available from Affymetrix (Santa Clara, CA, USA). .. Briefly, at least 5mg of purified RNA was reverse transcribed using Superscript II reverse transcriptase (Life Technologies, Grand Island, NY, USA) with the primer T7-dT24 containing a T7 RNA polymerase promoter.

    Article Title: Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
    Article Snippet: Arrays were scanned with the Affymetrix scanner (model 3000) with 7G upgrade, and data were collected using the GeneChip operating software (GCOS v3.1). .. CEL file output from Affymetrix microarray processing was uploaded, normalized within each array using endogenous expression and array exposure controls, and filtered if excessive missing values or outliers were detected. .. Probe set summaries and annotations were abstracted from standard annotation files specific to Affymetrix Human Exon 1.0 ST arrays ( http://www.affymetrix.com/support/technical/byproduct.affx?product=huexon-st ).


    Article Title: Type I IFNs Regulate Inflammation, Vasculopathy, and Fibrosis in Chronic Cutaneous Graft-versus-Host Disease.
    Article Snippet: .. Affymetrix (Santa Clara, CA) GeneChip Mouse Genome 430 2.0 microarray processing was carried out as outlined in the Affymetrix GeneChip manual. ..

    Article Title: CTGF Associated with Oncogenic Activities and Drug Resistance in Glioblastoma Multiforme (GBM)
    Article Snippet: Microarray procedures Vector control GBM cells (U343-V) and CTGF stably transfected cells (U343-CTGF: A combination of 50% U343-CTFG-1 and 50% U343-CTGF-2) were harvested and total RNA was extracted using RNeasy purification kit (Qiagen Inc., Valencia, CA) following the manufacturer’s advice. .. The detailed protocol for the sample preparation and microarray processing is available from Affymetrix Inc. (Santa Clara, CA). .. Biotinylated cRNA was hybridized to a HG-U133A microarray (Affymetrix Inc.).

    Sample Prep:

    Article Title: In vivo gene expression profile of guided bone regeneration associated with a microrough titanium surface.
    Article Snippet: Objectives: To determine the gene expression profile characteristic of ‘‘guided bone regeneration’’ associated with a microrough titanium surface.. Material and methods: Critical-size calvarial defects were treated with the principle of ‘‘guided bone regeneration,’’ whereby the extracranial barriers were either polished (SMO) or microrough (SLA) titanium disks.. After 7 and 14 days, the contents of the regenerating defect were collected, RNA was extracted and microarray analysis was carried out.

    Article Title: Therapeutic potential of MEK inhibition in acute myelogenous leukemia: rationale for "vertical" and "lateral" combination strategies.
    Article Snippet: In hematological malignancies, constitutive activation of the RAF/MEK/ERK pathway is frequently observed, conveys a poor prognosis, and constitutes a promising target for therapeutic intervention.. Here, we investigated the molecular and functional effects of pharmacological MEK inhibition in cell line models of acute myeloid leukemia (AML) and freshly isolated primary AML samples.. The small-molecule, ATP-non-competitive, MEK inhibitor PD0325901 markedly inhibited ERK phosphorylation and growth of several AML cell lines and approximately 70 % of primary AML samples.

    Article Title: CD52 as a molecular target for immunotherapy to treat acute myeloid leukemia with high EVI1 expression.
    Article Snippet: The antibody against caspase-3 was commercially obtained from Cell Signaling Technologies (Beverly, MA, USA; catalog no. 9251). .. The protocol used for the sample preparation and microarray processing is available from Affymetrix (Santa Clara, CA, USA). .. Briefly, at least 5mg of purified RNA was reverse transcribed using Superscript II reverse transcriptase (Life Technologies, Grand Island, NY, USA) with the primer T7-dT24 containing a T7 RNA polymerase promoter.

    Article Title: CTGF Associated with Oncogenic Activities and Drug Resistance in Glioblastoma Multiforme (GBM)
    Article Snippet: Microarray procedures Vector control GBM cells (U343-V) and CTGF stably transfected cells (U343-CTGF: A combination of 50% U343-CTFG-1 and 50% U343-CTGF-2) were harvested and total RNA was extracted using RNeasy purification kit (Qiagen Inc., Valencia, CA) following the manufacturer’s advice. .. The detailed protocol for the sample preparation and microarray processing is available from Affymetrix Inc. (Santa Clara, CA). .. Biotinylated cRNA was hybridized to a HG-U133A microarray (Affymetrix Inc.).

    other:

    Article Title: Comparative study of the transcriptomes of Caco-2 cells cultured under dynamic vs. static conditions following exposure to titanium dioxide and zinc oxide nanomaterials.
    Article Snippet: Affymetrix microarray processing and analysis The isolated RNA was subjected to genome-wide expression profiling.

    Expressing:

    Article Title: Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
    Article Snippet: Arrays were scanned with the Affymetrix scanner (model 3000) with 7G upgrade, and data were collected using the GeneChip operating software (GCOS v3.1). .. CEL file output from Affymetrix microarray processing was uploaded, normalized within each array using endogenous expression and array exposure controls, and filtered if excessive missing values or outliers were detected. .. Probe set summaries and annotations were abstracted from standard annotation files specific to Affymetrix Human Exon 1.0 ST arrays ( http://www.affymetrix.com/support/technical/byproduct.affx?product=huexon-st ).



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    Ribobio co microarray processing and analysis for mirs
    Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) <t>Microarray</t> and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.
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    Image Search Results


    Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) Microarray and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.

    Journal: International Journal of Biological Sciences

    Article Title: Upregulation of miR-335-3p by NF-κB Transcriptional Regulation Contributes to the Induction of Pulmonary Arterial Hypertension via APJ during Hypoxia

    doi: 10.7150/ijbs.34517

    Figure Lengend Snippet: Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) Microarray and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.

    Article Snippet: Microarray processing and analysis for miRs was performed by the Ribobio Co., Ltd. (Guangzhou, China).

    Techniques: Expressing, Microarray, Quantitative RT-PCR