rotating microarray hybridization oven (Agilent technologies)
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Agilent technologies
rotating microarray hybridization oven
Rotating Microarray Hybridization Oven, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+oven/pmc11054952-207-31-34
Average 90 stars, based on 1 article reviews
Rotating Microarray Hybridization Oven, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+oven/pmc11054952-207-31-34
Average 90 stars, based on 1 article reviews
rotating microarray hybridization oven - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes"
Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
Journal: Non-Coding RNA
doi: 10.3390/ncrna10020025
Figure Legend Snippet: Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
Techniques Used: Microarray, Software
Figure Legend Snippet: Top ten downregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
Techniques Used: Microarray, Software
Figure Legend Snippet: Difference in lncRNA and mRNA fold-changes between the cDNA microarray and in-house RT-qPCR experiments. Data shown are from three independent experiments (in triplicates) with calculated SD values to represent error bars and their statistical analyses were conducted using one-way ANOVA.
Techniques Used: Microarray, Quantitative RT-PCR
Figure Legend Snippet: ( A ) LncRNA-mRNA interaction network was constructed based on the ten selected candidate lncRNAs from the cDNA microarray dataset. The resulting mRNA interactions were predicted using the “rtool” database with −20 kcal as the minimum energy threshold and were filtered to show only mRNAs that were also dysregulated in the dataset. Data were visualized using Cytoscape v3.8.2. (Pink diamond = candidate lncRNA, blue circle = predicted putative mRNA targets). ( B ) A total of nine mRNAs were identified as both responsible in the “regulated exocytosis” hit as well as highly likely to be regulated by the candidate lncRNAs listed in ( A ). Data were visualized using Cytoscape v3.8.2. (pink diamond = candidate regulator lncRNA, blue circle = mRNA predicted involved in the biological process).
Techniques Used: Construct, Microarray
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After fragmentation steps following the manufacturer’s protocol, the cRNAs were immediately hybridized into the Agilent SurePrint G3 Human Gene Expression v3 8x60K for 17 h at 65 °C in a rotating Microarray:Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes Article Snippet: Cy3-labeled cRNA was then purified using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) to purify the amplified cRNA samples before dye incorporation and cRNA yield was assessed via NanoVueTM Plus Spectrophotometer (GE Healthcare, Chicago, IL, USA). .. 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Recent studies have highlighted neuronal apoptosis and abnormal dendritic structures as crucial features of PND.. Astrocytes-derived exosomes (ADEs) have been identified as carriers of microRNAs (miRNAs), playing a vital role in cell-to-cell communication through transmitting genetic material. Article Title: Community assembly of organisms regulates soil microbial functional potential through dual mechanisms. Article Snippet: 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China 2College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, China 3Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, China 4Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China 5College of Land and Environment, Shenyang Agricultural University, Shengyang, China 6Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China 7Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, China 8Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China 9Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, China 10Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA Article Title: Mpox infection protects against re-challenge in rhesus macaques. Article Snippet: The modified aptamer binding reagents, SomaScan Assay, its performance characteristics, and specificity to human targets have been previously described.28 The assay used standard controls, including 12 hybridization normalization control sequences used to control for variability in the Agilent microarray readout Cell 186, 4652–4661.e1–e5, October 12, 2023 e4 ll OPEN ACCESS Article process, as well as five human calibrator control pooled serum replicates and 3 Quality Control (QC) pooled replicates used to mitigate batch effects and verify the quality of the assay run using standard acceptance criteria. .. The readout is performed using Article Title: Deciphering the Functional Analysis of Differentially Expressed MicroRNAs Associated with Colorectal Cancer Article Snippet: The labelled miRNA was then mixed with 10 Hi-RPM Hybridization buffer, 2 Hi-RPM Page 4/20 Hybridization spike-in solution, and 2 Agilent blocking agent. .. 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Using comparative transcriptomics, namely Labeling:Article Title: Astrocyte-derived exosomes-transported miRNA-26a-5p ameliorates sevoflurane-induced cognitive dysfunction in aged mice. Article Snippet: Prolonged sevoflurane anesthesia is the primary factor contributing to the development of perioperative neurocognitive disorders (PND).. Recent studies have highlighted neuronal apoptosis and abnormal dendritic structures as crucial features of PND.. Astrocytes-derived exosomes (ADEs) have been identified as carriers of microRNAs (miRNAs), playing a vital role in cell-to-cell communication through transmitting genetic material. Article Title: Community assembly of organisms regulates soil microbial functional potential through dual mechanisms. Article Snippet: 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China 2College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, China 3Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, China 4Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China 5College of Land and Environment, Shenyang Agricultural University, Shengyang, China 6Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China 7Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, China 8Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China 9Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, China 10Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA Extraction:Article Title: Mpox infection protects against re-challenge in rhesus macaques. Article Snippet: The modified aptamer binding reagents, SomaScan Assay, its performance characteristics, and specificity to human targets have been previously described.28 The assay used standard controls, including 12 hybridization normalization control sequences used to control for variability in the Agilent microarray readout Cell 186, 4652–4661.e1–e5, October 12, 2023 e4 ll OPEN ACCESS Article process, as well as five human calibrator control pooled serum replicates and 3 Quality Control (QC) pooled replicates used to mitigate batch effects and verify the quality of the assay run using standard acceptance criteria. .. The readout is performed using Incubation:Article Title: Deciphering the Functional Analysis of Differentially Expressed MicroRNAs Associated with Colorectal Cancer Article Snippet: The labelled miRNA was then mixed with 10 Hi-RPM Hybridization buffer, 2 Hi-RPM Page 4/20 Hybridization spike-in solution, and 2 Agilent blocking agent. .. After incubation at 100°C, samples are immediately hybridised to Agilent SurePrint G3 Human miRNA Microarray 860K (Design ID: 070156) for 20 hours at 55°C in a rotating |
