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Corning Life Sciences microarray hybridization chamber
Microarray Hybridization Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+chamber/microarray+hybridization+chambers/pmc01435976-68-34-40
Average 90 stars, based on 1 article reviews
microarray hybridization chamber - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Hybridization:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: Gene expression and pathologic alterations in juvenile rainbow trout due to chronic dietary TCDD exposure
Article Snippet: .. Hybridization was performed using HybriSlips coverslips (Sigma, St. Louis, MO) and Corning microarray hybridization chambers (Corning, Tewksbury, MA) in a water bath at 50°C for 16 h; the first hybridization used LNA dT blocker (vial 9 in the Array 900 kit) and formamide-based hybridization buffer (vial 7 in the Array 900 kit) as directed in the Array 900 manual. .. Following the first hybridization, arrays were washed in 2× SSC (Ambion, Austin, TX), 0.2% SDS solution (Ambion, Austin, TX) at 50°C (1 × 15 min), 2× SSC (1 × 15 min) at room temperature, then 0.2× SSC (1 × 15 min) at room temperature; all microarray wash solutions were made using RNase-free water.

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia
Article Snippet: .. Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA). .. Hybridization and wash buffers from the miRCURY LNA microRNA Array Kit (Exiqon) were used according to the manufacturer’s instructions.

Article Title: Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids
Article Snippet: .. Hybridisation was performed overnight in a 42 °C water bath in Corning microarray hybridization chambers. ..

Article Title: Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates.
Article Snippet: A novel immobilization approach involving binding of preformed streptavidin/biotinylated oligonucleotide conjugates onto surfaces coated with biotinylated bovine serum albumin is presented.. Microarrays prepared according to the proposed method were compared, in terms of detection sensitivity and specificity, with other immobilization schemes employing coupling of biotinylated oligonucleotides onto directly adsorbed surface streptavidin, or sequential coupling of streptavidin and biotinylated oligonucleotides onto a layer of adsorbed biotinylated bovine serum albumin.. A comparison was performed employing biotinylated oligonucleotides corresponding to wildand mutant-type sequences of seven single point mutations of the BRCA1 gene.

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Microarray:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: Gene expression and pathologic alterations in juvenile rainbow trout due to chronic dietary TCDD exposure
Article Snippet: .. Hybridization was performed using HybriSlips coverslips (Sigma, St. Louis, MO) and Corning microarray hybridization chambers (Corning, Tewksbury, MA) in a water bath at 50°C for 16 h; the first hybridization used LNA dT blocker (vial 9 in the Array 900 kit) and formamide-based hybridization buffer (vial 7 in the Array 900 kit) as directed in the Array 900 manual. .. Following the first hybridization, arrays were washed in 2× SSC (Ambion, Austin, TX), 0.2% SDS solution (Ambion, Austin, TX) at 50°C (1 × 15 min), 2× SSC (1 × 15 min) at room temperature, then 0.2× SSC (1 × 15 min) at room temperature; all microarray wash solutions were made using RNase-free water.

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia
Article Snippet: .. Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA). .. Hybridization and wash buffers from the miRCURY LNA microRNA Array Kit (Exiqon) were used according to the manufacturer’s instructions.

Article Title: Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids
Article Snippet: .. Hybridisation was performed overnight in a 42 °C water bath in Corning microarray hybridization chambers. ..

Article Title: Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates.
Article Snippet: A novel immobilization approach involving binding of preformed streptavidin/biotinylated oligonucleotide conjugates onto surfaces coated with biotinylated bovine serum albumin is presented.. Microarrays prepared according to the proposed method were compared, in terms of detection sensitivity and specificity, with other immobilization schemes employing coupling of biotinylated oligonucleotides onto directly adsorbed surface streptavidin, or sequential coupling of streptavidin and biotinylated oligonucleotides onto a layer of adsorbed biotinylated bovine serum albumin.. A comparison was performed employing biotinylated oligonucleotides corresponding to wildand mutant-type sequences of seven single point mutations of the BRCA1 gene.

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Labeling:

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Purification:

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Incubation:

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..



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Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide <t>microarray</t> data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
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Image Search Results


Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Journal: BMC Cancer

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

doi: 10.1186/1471-2407-13-364

Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

Techniques: Microarray, Quantitative RT-PCR, Expressing

A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

Journal: PLoS ONE

Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression

doi: 10.1371/journal.pone.0110934

Figure Lengend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

Article Snippet: Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008).

Techniques: Expressing, Microarray, Derivative Assay