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microarray hybridisation chambers  (Agilent technologies)


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    Structured Review

    Agilent technologies microarray hybridisation chambers
    Microarray Hybridisation Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridisation+chambers/pm19150360-209-5-8
    Average 90 stars, based on 1 article reviews
    microarray hybridisation chambers - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Transcript elongation factor TFIIS is involved in arabidopsis seed dormancy.
    Article Snippet: 0022-2836/$ see front matter © 2008 E Transcript elongation factor TFIIS promotes efficient transcription by RNA polymerase II, since it assists in bypassing blocks during mRNA synthesis.. While yeast cells lacking TFIIS are viable, inactivation of mouse TFIIS causes embryonic lethality.. Here, we have identified a protein encoded in the Arabidopsis genome that displays a marked sequence similarity to TFIIS of other organisms, primarily within domains II and III in the C-terminal part of the protein.

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Article Title: High affinity synbodies for influenza
    Article Snippet: .. Influenza solutions (250 uL) at five different concentrations (7.5×108, 1.5×109, 3×109, 7.5×109, 1.5×1010 vp/ml) were incubated in competition with protease inhibited bovine serum (5 mg/mL) in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed as before and probed with 5 nM, 250 uL mouse monoclonal anti-influenza NA (BEI Resources, Cat. No: NR-4540) with same competitor in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed and probed with 5 nM, 250 uL AlexaFluor-647 conjugated anti-mouse secondary antibody (Life Technologies, Cat. No: A21235) for 1 hour at 37° C. All the slides were washed, dried, and scanned on an Agilent Microarray Scanner. ..

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Hybridization:

    Article Title: Transcript elongation factor TFIIS is involved in arabidopsis seed dormancy.
    Article Snippet: 0022-2836/$ see front matter © 2008 E Transcript elongation factor TFIIS promotes efficient transcription by RNA polymerase II, since it assists in bypassing blocks during mRNA synthesis.. While yeast cells lacking TFIIS are viable, inactivation of mouse TFIIS causes embryonic lethality.. Here, we have identified a protein encoded in the Arabidopsis genome that displays a marked sequence similarity to TFIIS of other organisms, primarily within domains II and III in the C-terminal part of the protein.

    Virus:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Protease Inhibitor:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Peptide Microarray:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Incubation:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Article Title: High affinity synbodies for influenza
    Article Snippet: .. Influenza solutions (250 uL) at five different concentrations (7.5×108, 1.5×109, 3×109, 7.5×109, 1.5×1010 vp/ml) were incubated in competition with protease inhibited bovine serum (5 mg/mL) in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed as before and probed with 5 nM, 250 uL mouse monoclonal anti-influenza NA (BEI Resources, Cat. No: NR-4540) with same competitor in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed and probed with 5 nM, 250 uL AlexaFluor-647 conjugated anti-mouse secondary antibody (Life Technologies, Cat. No: A21235) for 1 hour at 37° C. All the slides were washed, dried, and scanned on an Agilent Microarray Scanner. ..

    Labeling:

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Control:

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Software:

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.



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    Agilent technologies microarray hybridisation chamber kit
    (A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
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    Agilent technologies microarray hybridisation chambers
    (A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
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    Image Search Results


    (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

    Journal: bioRxiv

    Article Title: Genotype-phenotype map of an RNA-ligand complex

    doi: 10.1101/2020.12.17.423258

    Figure Lengend Snippet: (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

    Article Snippet: 40 μl of the hybridisation mix was transferred into each of eight wells on a hybridisation gasket slide (Agilent, G2534-60015) during hybridisation assembly using the Agilent Microarray Hybridisation Chamber Kit (Agilent, Cat No. G2534A) and following the standard protocol.

    Techniques: Microarray, Fluorescence, Concentration Assay, Standard Deviation