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gene expression microarray experiment  (Thermo Fisher)


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    Thermo Fisher gene expression microarray experiment
    Gene Expression Microarray Experiment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+gene+expression+experiments/pmc05782396-239-15-14
    Average 86 stars, based on 1 article reviews
    gene expression microarray experiment - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Post-Transcriptional Regulation of BCL2 mRNA by the RNA-Binding Protein ZFP36L1 in Malignant B Cells
    Article Snippet: .. To identify mRNAs that are regulated by ZFP36 family members at a global level, a gene expression microarray dataset comprised of 206 cell line samples representing cell types of diverse histological origin was downloaded from NCBI Gene Expression Omnibus database (Affymetrix; GSE10843, http://www.ncbi.nlm.nih.gov/geo ). ..

    Article Title: Structural and Regulatory Characterization of the Placental Epigenome at Its Maternal Interface
    Article Snippet: Given the potential for DNA methylation patterns to be intimately associated with gene expression, we sought to determine whether T-DMRs identified in the Illumina data were also present as tissue specific differentially transcribed genes (TDTs). .. Thus, we analyzed Affymetrix gene expression microarray data to identify CVS- and MBC-specific TDTs and then analyzed these data using IPA. ..

    Article Title: Celsius: a community resource for Affymetrix microarray data
    Article Snippet: .. Although gene expression microarray technology has been implemented in a variety of formats (spotted cDNAs, spotted column-synthesized oligos, and in situ synthesized oligos), the leading commercial supplier of microarrays has been Affymetrix Inc. (Santa Clara, CA, USA) since 1996. .. Within the GEO repository Affymetrix platforms account for 35% of all arrays deposited, but they represent approximately 60% of the genome-scale gene expression data.

    Article Title: Workflows for microarray data processing in the Kepler environment
    Article Snippet: .. For Affymetrix gene expression microarray processing, there are two closely related workflows, differing by differential gene expression determination methodology. ..

    Article Title: Integrated Genomic Analysis of Sézary Syndrome
    Article Snippet: Then we imported these 9 gene-lists into GeneSpring software version 7 ( http://www.sigenetics.com/ ), which contained a genome/raw data of gene expression profile from 6 SS patients who were treated with photopheresis therapy and 2 healthy individuals generated by using Affymetrix GeneChip U133A ( http://www.affymetrix.com/products/arrays/specific/hgu133av2.affx ). .. Of 6 SS cases analysed with Affymetrix gene expression microarray the 3 cases were also tested by above Affymetrix SNP microarray. ..

    Article Title: SplicerAV: a tool for mining microarray expression data for changes in RNA processing
    Article Snippet: .. To fully investigate the potential to detect isoform-specific mRNA changes in conventional gene expression microarray data, we elected to develop a novel method, SplicerAV, which we have applied to conventional Affymetrix gene expression microarray data. .. For the Affymetrix GeneChip Human U133 plus 2.0 arrays, 11,193 genes, which represent 57% of uniquely annotated genes assayed by the array, are interrogated by multiple probesets and can therefore be queried for mRNA isoform changes, with an average of 3.2 probesets interrogating these genes (Table ).

    Article Title: Transcription Factors MYOCD, SRF, Mesp1 and SMARCD3 Enhance the Cardio-Inducing Effect of GATA4, TBX5, and MEF2C during Direct Cellular Reprogramming
    Article Snippet: .. Relative fold change for these genes was calculated using published Affymetrix microarray gene expression data ( ). ..

    Article Title: Data Integration in Genetics and Genomics: Methods and Challenges
    Article Snippet: .. For example, different approaches are utilized for preprocessing cDNA and Affymetrix gene expression microarray data [ , ]. ..

    Microarray:

    Article Title: Post-Transcriptional Regulation of BCL2 mRNA by the RNA-Binding Protein ZFP36L1 in Malignant B Cells
    Article Snippet: .. To identify mRNAs that are regulated by ZFP36 family members at a global level, a gene expression microarray dataset comprised of 206 cell line samples representing cell types of diverse histological origin was downloaded from NCBI Gene Expression Omnibus database (Affymetrix; GSE10843, http://www.ncbi.nlm.nih.gov/geo ). ..

    Article Title: Structural and Regulatory Characterization of the Placental Epigenome at Its Maternal Interface
    Article Snippet: Given the potential for DNA methylation patterns to be intimately associated with gene expression, we sought to determine whether T-DMRs identified in the Illumina data were also present as tissue specific differentially transcribed genes (TDTs). .. Thus, we analyzed Affymetrix gene expression microarray data to identify CVS- and MBC-specific TDTs and then analyzed these data using IPA. ..

    Article Title: Celsius: a community resource for Affymetrix microarray data
    Article Snippet: .. Although gene expression microarray technology has been implemented in a variety of formats (spotted cDNAs, spotted column-synthesized oligos, and in situ synthesized oligos), the leading commercial supplier of microarrays has been Affymetrix Inc. (Santa Clara, CA, USA) since 1996. .. Within the GEO repository Affymetrix platforms account for 35% of all arrays deposited, but they represent approximately 60% of the genome-scale gene expression data.

    Article Title: Workflows for microarray data processing in the Kepler environment
    Article Snippet: .. For Affymetrix gene expression microarray processing, there are two closely related workflows, differing by differential gene expression determination methodology. ..

    Article Title: Integrated Genomic Analysis of Sézary Syndrome
    Article Snippet: Then we imported these 9 gene-lists into GeneSpring software version 7 ( http://www.sigenetics.com/ ), which contained a genome/raw data of gene expression profile from 6 SS patients who were treated with photopheresis therapy and 2 healthy individuals generated by using Affymetrix GeneChip U133A ( http://www.affymetrix.com/products/arrays/specific/hgu133av2.affx ). .. Of 6 SS cases analysed with Affymetrix gene expression microarray the 3 cases were also tested by above Affymetrix SNP microarray. ..

    Article Title: SplicerAV: a tool for mining microarray expression data for changes in RNA processing
    Article Snippet: .. To fully investigate the potential to detect isoform-specific mRNA changes in conventional gene expression microarray data, we elected to develop a novel method, SplicerAV, which we have applied to conventional Affymetrix gene expression microarray data. .. For the Affymetrix GeneChip Human U133 plus 2.0 arrays, 11,193 genes, which represent 57% of uniquely annotated genes assayed by the array, are interrogated by multiple probesets and can therefore be queried for mRNA isoform changes, with an average of 3.2 probesets interrogating these genes (Table ).

    Article Title: Transcription Factors MYOCD, SRF, Mesp1 and SMARCD3 Enhance the Cardio-Inducing Effect of GATA4, TBX5, and MEF2C during Direct Cellular Reprogramming
    Article Snippet: .. Relative fold change for these genes was calculated using published Affymetrix microarray gene expression data ( ). ..

    Article Title: Data Integration in Genetics and Genomics: Methods and Challenges
    Article Snippet: .. For example, different approaches are utilized for preprocessing cDNA and Affymetrix gene expression microarray data [ , ]. ..

    Indirect Immunoperoxidase Assay:

    Article Title: Structural and Regulatory Characterization of the Placental Epigenome at Its Maternal Interface
    Article Snippet: Given the potential for DNA methylation patterns to be intimately associated with gene expression, we sought to determine whether T-DMRs identified in the Illumina data were also present as tissue specific differentially transcribed genes (TDTs). .. Thus, we analyzed Affymetrix gene expression microarray data to identify CVS- and MBC-specific TDTs and then analyzed these data using IPA. ..

    In Situ:

    Article Title: Celsius: a community resource for Affymetrix microarray data
    Article Snippet: .. Although gene expression microarray technology has been implemented in a variety of formats (spotted cDNAs, spotted column-synthesized oligos, and in situ synthesized oligos), the leading commercial supplier of microarrays has been Affymetrix Inc. (Santa Clara, CA, USA) since 1996. .. Within the GEO repository Affymetrix platforms account for 35% of all arrays deposited, but they represent approximately 60% of the genome-scale gene expression data.

    Synthesized:

    Article Title: Celsius: a community resource for Affymetrix microarray data
    Article Snippet: .. Although gene expression microarray technology has been implemented in a variety of formats (spotted cDNAs, spotted column-synthesized oligos, and in situ synthesized oligos), the leading commercial supplier of microarrays has been Affymetrix Inc. (Santa Clara, CA, USA) since 1996. .. Within the GEO repository Affymetrix platforms account for 35% of all arrays deposited, but they represent approximately 60% of the genome-scale gene expression data.



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    ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from <t>microarray</t> data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.
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    Thermo Fisher gene expression microarray experiment
    ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from <t>microarray</t> data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.
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    ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from <t>microarray</t> data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.
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    ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from <t>microarray</t> data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.
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    Image Search Results


    ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

    Journal: International Journal of Medical Sciences

    Article Title: Long noncoding RNA ZEB1-AS1 acts as a Sponge of miR-141-3p to Inhibit Cell Proliferation in Colorectal Cancer

    doi: 10.7150/ijms.46698

    Figure Lengend Snippet: ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

    Article Snippet: Shanghai Biotechnology Co., Ltd conducted the microRNA microarray gene expression experiments and data analysis.

    Techniques: Fractionation, Expressing, Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Assay, Activity Assay

    ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

    Journal: Journal of Cancer

    Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2

    doi: 10.7150/jca.34886

    Figure Lengend Snippet: ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

    Article Snippet: TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service.

    Techniques: Microarray, Expressing, Control, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Functional Assay, Activity Assay, Fluorescence, Flow Cytometry