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LC Sciences sequencing and data analysis
Sequencing And Data Analysis, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Dural Tregs driven by astrocytic IL-33 mitigate depression through the EGFR signals in mPFC neurons.
Article Snippet: The dura sinus-resident immune cells can influence the process of central neural system (CNS) diseases by communicating with central nerve cells.. In clinical, Tregs are also frequently impaired in depression.. However, the significance of this relationship remains unknown.

Article Title: Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals
Article Snippet: The nitrocellulose membrane was cut, and then digested with proteinase K. The recovered RNA was purified, and then sequencing and data analysis were performed by LC sciences (Hangzhou, China).

Microarray:

Article Title: microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri.
Article Snippet: Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity.. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus.. In this article, we identified the amphioxus miRNAs in the acute-phase response to lipopolysaccharide (LPS).

Article Title: Reprograming of epigenetic mechanisms controlling host insect immunity and development in response to egg-laying by a parasitoid wasp
Article Snippet: .. Microarray hybridization, detection and analysis were carried out by LC Sciences (Houston, TX, USA) as previously described [ 28 ]. .. Target mRNAs were identification in silico by complementarity to miRNA probes using the sequence alignment editor BioEdit v. 7.2.5 as previously described [ 11 ].

Article Title: Engineered microRNA-based regulatory element permits safe high-dose mini MECP2 gene therapy in Rett mice
Article Snippet: .. Microarray data from LC Sciences are provided within the Supplementary material . ..

Article Title: Disturbance of the Dlk1-Dio3 imprinted domain may underlie placental Dio3 suppression and extracellular thyroid hormone disturbance in placenta-derived JEG-3 cells following decabromodiphenyl ether (BDE209) exposure.
Article Snippet: Decabromodiphenyl ether (BDE209) has been widely used as a flame retardant in the past four decades, leading to human health consequences, especially neurological impairments.. Our previous in vivo studies have suggested that developmental neurotoxicity in offspring may be the result of BDE209-induced placental type III iodothyronine deiodinase (Dio3) disturbance and consequent thyroid hormone (TH) instability.. Dio3 is paternally imprinted gene, and its balanced expression is crucial in directing normal development and growth.

Article Title: Reprograming of epigenetic mechanisms controlling host insect immunity and development in response to egg-laying by a parasitoid wasp
Article Snippet: .. Microarray hybridization, detection and analysis were carried out by LC Sciences (Houston, TX, USA) as previously described [ 28 ]. (g) Prediction of miRNA targets Target mRNAs were identification in silico by complementarity to miRNA probes using the sequence alignment editor BioEdit v. 7.2.5 as previously described [ 11 ]. .. Briefly, we identified open reading frames (ORFs) in all contigs in the sequenced G. mellonella transcriptome using the ‘Find next ORF’ option in BioEdit.

Hybridization:

Article Title: Reprograming of epigenetic mechanisms controlling host insect immunity and development in response to egg-laying by a parasitoid wasp
Article Snippet: .. Microarray hybridization, detection and analysis were carried out by LC Sciences (Houston, TX, USA) as previously described [ 28 ]. .. Target mRNAs were identification in silico by complementarity to miRNA probes using the sequence alignment editor BioEdit v. 7.2.5 as previously described [ 11 ].

Article Title: Reprograming of epigenetic mechanisms controlling host insect immunity and development in response to egg-laying by a parasitoid wasp
Article Snippet: .. Microarray hybridization, detection and analysis were carried out by LC Sciences (Houston, TX, USA) as previously described [ 28 ]. (g) Prediction of miRNA targets Target mRNAs were identification in silico by complementarity to miRNA probes using the sequence alignment editor BioEdit v. 7.2.5 as previously described [ 11 ]. .. Briefly, we identified open reading frames (ORFs) in all contigs in the sequenced G. mellonella transcriptome using the ‘Find next ORF’ option in BioEdit.

In Silico:

Article Title: Reprograming of epigenetic mechanisms controlling host insect immunity and development in response to egg-laying by a parasitoid wasp
Article Snippet: .. Microarray hybridization, detection and analysis were carried out by LC Sciences (Houston, TX, USA) as previously described [ 28 ]. (g) Prediction of miRNA targets Target mRNAs were identification in silico by complementarity to miRNA probes using the sequence alignment editor BioEdit v. 7.2.5 as previously described [ 11 ]. .. Briefly, we identified open reading frames (ORFs) in all contigs in the sequenced G. mellonella transcriptome using the ‘Find next ORF’ option in BioEdit.

Sequencing:

Article Title: Reprograming of epigenetic mechanisms controlling host insect immunity and development in response to egg-laying by a parasitoid wasp
Article Snippet: .. Microarray hybridization, detection and analysis were carried out by LC Sciences (Houston, TX, USA) as previously described [ 28 ]. (g) Prediction of miRNA targets Target mRNAs were identification in silico by complementarity to miRNA probes using the sequence alignment editor BioEdit v. 7.2.5 as previously described [ 11 ]. .. Briefly, we identified open reading frames (ORFs) in all contigs in the sequenced G. mellonella transcriptome using the ‘Find next ORF’ option in BioEdit.



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Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

Journal: Blood

Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

doi: 10.1182/blood-2010-10-313106

Figure Lengend Snippet: Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

Techniques: Microarray, Expressing, Gene Expression

Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

Journal: Blood

Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

doi: 10.1182/blood-2010-10-313106

Figure Lengend Snippet: Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

Techniques: Microarray, Selection

Validation of  DNA   Microarray  Results by qPCR

Journal: Investigative Ophthalmology & Visual Science

Article Title: Perimysial Fibroblasts of Extraocular Muscle, as Unique as the Muscle Fibers

doi: 10.1167/iovs.08-2857

Figure Lengend Snippet: Validation of DNA Microarray Results by qPCR

Article Snippet: DNA Microarray Data Analysis Raw data from microarray scans were analyzed with microarray analysis software (GCOS 2.0; Affymetrix).

Techniques: Biomarker Discovery, Microarray