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microarray data analysis raw data  (Danaher Inc)


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    Structured Review

    Danaher Inc microarray data analysis raw data
    Microarray Data Analysis Raw Data, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+data+analysis+raw+data/GenePix+Microarray+Systems/pmc02075096-91-0-17
    Average 94 stars, based on 460 article reviews
    microarray data analysis raw data - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Microarray:

    Article Title: Unraveling the role of HDAC3 as an immunotherapy prognostic biomarker and therapeutic target in advanced non-small cell lung cancer
    Article Snippet: The microarray was retrieved from − 80 °C storage and blocked with bovine serum albumin (BSA, 9048-46-8, Sigma) for 1.5 h. Following the blocking step, the microarray was incubated with plasma samples at a 1:1000 dilution for 1 h. After washing with 0.1% phosphate-buffered saline-Tween 20, the plates were incubated with Alexa Fluor 647 goat anti-human IgG (109-605-003, Jackson) diluted in 5% BSA. .. The microarray was then scanned using a GenePix 4300 A microarray scanner (141095, Molecular Devices) with a 635 nm excitation laser. .. Signal intensities of IgG for each protein were quantified using GenePix Pro v.6.0 software (Molecular Devices).

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Article Title: Supporting Information _ Lee and Fan _ Yale
    Article Snippet: .. An Axon Genepix microarray scanner 4000B (Molecular Devices, USA) was used to image DRAQ5-stained cells (emitting 635 nm) on the STRQNW arrays. ..

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    other:

    Article Title: Development of Lamprey-derived Antibodies Against Human Blood Group Antigens.
    Article Snippet: A major challenge in the glycosciences is the scarcity of sensitive and specific glycanbinding reagents, such as monoclonal antibodies, for detecting and isolating glycans.. Here we report the development and characterization of new monoclonal antibodies (mAbs) that bind carbohydrate-based red blood cell (RBC) antigens including the ABO(H) antigens.. This approach exploits the immune system of the sea lamprey (Petromyzon marinus), which strongly responds to human glycans to enable the generation of high affinity antibodies.

    Article Title: Development of lamprey-derived antibodies against human blood group antigens
    Article Snippet: The scans were analyzed using GenePix (Molecular Devices) software and GLAD (GLycan Array Dashboard) software ( ).

    Binding Assay:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Incubation:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Software:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Fluorescence:

    Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development
    Article Snippet: After being washed three times and air-dried through brief centrifugation, the slides were scanned using a Genepix 4200A scanner (Molecular Devices, CA, USA). .. The median foreground and background fluorescence intensities were measured for each spot on the protein microarrays using GenePix Pro 6.0 (Molecular Devices, CA, USA). ..



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    Image Search Results


    Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

    Journal: Blood

    Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

    doi: 10.1182/blood-2010-10-313106

    Figure Lengend Snippet: Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

    Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

    Techniques: Microarray, Expressing, Gene Expression

    Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

    Journal: Blood

    Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

    doi: 10.1182/blood-2010-10-313106

    Figure Lengend Snippet: Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

    Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

    Techniques: Microarray, Selection

    Validation of  DNA   Microarray  Results by qPCR

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Perimysial Fibroblasts of Extraocular Muscle, as Unique as the Muscle Fibers

    doi: 10.1167/iovs.08-2857

    Figure Lengend Snippet: Validation of DNA Microarray Results by qPCR

    Article Snippet: DNA Microarray Data Analysis Raw data from microarray scans were analyzed with microarray analysis software (GCOS 2.0; Affymetrix).

    Techniques: Biomarker Discovery, Microarray