microarray analysis (BioCore Inc)
Structured Review
![(A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using <t>microarray</t> data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3866/pmc06743866/pmc06743866__pone.0222559.g003.jpg)
Microarray Analysis, supplied by BioCore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+data+analysis+raw+data/microarray+data+analysis/pmc06743866-53-1-16
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation"
Article Title: Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation
Journal: PLoS ONE
doi: 10.1371/journal.pone.0222559
Figure Legend Snippet: (A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using microarray data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).
Techniques Used: Modification, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, SYBR Green Assay
Related Articles
Microarray:Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer Article Snippet: .. Article Title: Investigating miR-6880-5p in extracellular vesicle from plasma as a prognostic biomarker in endocrine therapy-treated castration-resistant prostate cancer. Article Snippet: .. The isolated total EV-RNAs were sent for Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Article Title: Circulating microRNA-423 attenuates the phosphorylation of calcium handling proteins in atrial fibrillation Article Snippet: Image processing was performed using an Affymetrix Gene Array 3000 scanner, and data analysis was performed using GeneSpring GX 14.9.1 software. .. Quality control and Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer. Article Snippet: Department of Oncology, University of Wisconsin, Madison, WI, 53705, USA Carbone Cancer Center, University of Wisconsin, Madison, WI, 53705, USA Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, 53705, USA William S. Middleton VA Medical Center, Madison, WI, 53705, USA Department of Statistics, University of Wisconsin, Madison, WI, 53706, USA Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI, 53792, USA Division of Hematology, Medical Oncology, and Palliative Care, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Department of Internal Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA Department of Radiology, University of Wisconsin, Madison, WI, 53705, USA Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA Article Title: Stromal Versican Accumulation and Proteolysis Regulate the Infiltration of CD8 + T Cells in Breast Cancer Article Snippet: .. A total of 371 cases of invasive breast cancer were identified and used to generate a Formalin-fixed Paraffin-Embedded:Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer Article Snippet: .. Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer. Article Snippet: Department of Oncology, University of Wisconsin, Madison, WI, 53705, USA Carbone Cancer Center, University of Wisconsin, Madison, WI, 53705, USA Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, 53705, USA William S. Middleton VA Medical Center, Madison, WI, 53705, USA Department of Statistics, University of Wisconsin, Madison, WI, 53706, USA Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI, 53792, USA Division of Hematology, Medical Oncology, and Palliative Care, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Department of Internal Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA Department of Radiology, University of Wisconsin, Madison, WI, 53705, USA Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA other:Article Title: Mesenchymal stem cell-derived exosomes suppress proliferation of T cells by inducing cell cycle arrest through p27kip1/Cdk2 signaling. Article Snippet: Mouse mesenchymal stem cells (MSCs) have been shown to suppress T cells.. Especially, MSC-cultured media have shown suppressive functions against various immune cells including the T cells.. However, the underlying immunosuppressive mechanisms of the MSC-cultured medium are not yet fully understood. Isolation:Article Title: Investigating miR-6880-5p in extracellular vesicle from plasma as a prognostic biomarker in endocrine therapy-treated castration-resistant prostate cancer. Article Snippet: .. The isolated total EV-RNAs were sent for Multiple Displacement Amplification:Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Expressing:Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Control:Article Title: Circulating microRNA-423 attenuates the phosphorylation of calcium handling proteins in atrial fibrillation Article Snippet: Image processing was performed using an Affymetrix Gene Array 3000 scanner, and data analysis was performed using GeneSpring GX 14.9.1 software. .. Quality control and |

