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Structured Review

BioCore Inc microarray analysis
(A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using <t>microarray</t> data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).
Microarray Analysis, supplied by BioCore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+data+analysis+raw+data/microarray+data+analysis/pmc06743866-53-1-16
Average 90 stars, based on 1 article reviews
microarray analysis - by Bioz Stars, 2026-09
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Images

1) Product Images from "Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation"

Article Title: Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0222559

(A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using microarray data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).
Figure Legend Snippet: (A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using microarray data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).

Techniques Used: Modification, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, SYBR Green Assay

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Formalin-fixed Paraffin-Embedded:

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Article Snippet: .. Colorectal cancer tissue microarray (CRC TMA) A formalin-fixed paraffin-embedded (FFPE) CRC TMA was developed by the University of Wisconsin Carbone Cancer Center Translational Science Biocore (TSB) under an IRB-approved protocol (2016–0934) as previously described [ 15 ]. ..

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other:

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Isolation:

Article Title: Investigating miR-6880-5p in extracellular vesicle from plasma as a prognostic biomarker in endocrine therapy-treated castration-resistant prostate cancer.
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Multiple Displacement Amplification:

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer
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Expressing:

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer
Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Affymetrix expression microarray analysis of the Hs578T cell line was performed by BioCore. ..

Control:

Article Title: Circulating microRNA-423 attenuates the phosphorylation of calcium handling proteins in atrial fibrillation
Article Snippet: Image processing was performed using an Affymetrix Gene Array 3000 scanner, and data analysis was performed using GeneSpring GX 14.9.1 software. .. Quality control and microarray analysis of small RNA experiments were performed at Biocore (Seoul, Korea). ..



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Image Search Results


Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

Journal: Blood

Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

doi: 10.1182/blood-2010-10-313106

Figure Lengend Snippet: Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

Techniques: Microarray, Expressing, Gene Expression

Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

Journal: Blood

Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

doi: 10.1182/blood-2010-10-313106

Figure Lengend Snippet: Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

Techniques: Microarray, Selection

Validation of  DNA   Microarray  Results by qPCR

Journal: Investigative Ophthalmology & Visual Science

Article Title: Perimysial Fibroblasts of Extraocular Muscle, as Unique as the Muscle Fibers

doi: 10.1167/iovs.08-2857

Figure Lengend Snippet: Validation of DNA Microarray Results by qPCR

Article Snippet: DNA Microarray Data Analysis Raw data from microarray scans were analyzed with microarray analysis software (GCOS 2.0; Affymetrix).

Techniques: Biomarker Discovery, Microarray